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• W2316766645 abstract "A solution additive has been discovered that can be used to measure the number of basic sites in a peptide or protein using electrospray ionization (ESI) mass spectrometry. Addition of millimolar amounts of perchloric acid (HClO4) to aqueous solutions that contain peptides or proteins results in the noncovalent adduction of HClO4 molecules to the multiply charged ions formed by ESI. For 18 oligopeptides and proteins, ranging in molecular weight from 0.5 to 18.3 kDa, the sum of the number of protons plus maximum number of HClO4 molecules adducted to the lower charge state ions is equal to the number of basic sites in the molecule. This method provides a rapid means of obtaining information about the composition of a peptide or protein and does not require high-resolution measurements or any instrumental or experimental modifications." @default.
• W2316766645 created "2016-06-24" @default.
• W2316766645 creator A5014296444 @default.
• W2316766645 creator A5032933084 @default.
• W2316766645 creator A5074015884 @default.
• W2316766645 date "2011-02-21" @default.
• W2316766645 modified "2023-10-10" @default.
• W2316766645 title "A Simple and Robust Method for Determining the Number of Basic Sites in Peptides and Proteins Using Electrospray Ionization Mass Spectrometry" @default.
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• W2316766645 doi "https://doi.org/10.1021/ac1031012" @default.
• W2316766645 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3089436" @default.
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• W2316766645 hasPublicationYear "2011" @default.
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