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- W2316973726 abstract "To examine hammerhead ribozyme activity in the nuclear environment, we have used nuclei isolated from HTLV-I tax transformed fibroblasts to evaluate ribozymes targeted against HTLV-I tax RNA. The ribozyme activity in nuclei suspension was strongly dependent on the resistance of the particular ribozyme to endogenous nucleases. A ribozyme containing exclusively 2′-deoxynucleotides in its stems cleaved target RNA by its catalytic activity in the absence of proteins and caused degradation in their presence by induction of nuclear RNase H activity. A ribozyme containing 2′-amino- and 2′-fluoropyrimidine nucleosides in combination with terminal phosphorothioate linkages was significantly more stable in nuclei suspension and also exhibited a more than threefold higher cleavage efficacy than its unmodified counterpart. The increased resistance against nuclease degradation is mainly due to terminal phosphorothioate linkages, suggesting that both 5′ and 3′-exonucleases are primarily responsible for the nuclear degradation of oligonucleotides." @default.
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- W2316973726 date "1996-01-01" @default.
- W2316973726 modified "2023-09-23" @default.
- W2316973726 title "Correlation of Activity with Stability of Chemically Modified Ribozymes in Nuclei Suspension" @default.
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- W2316973726 doi "https://doi.org/10.1089/oli.1.1996.6.111" @default.
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