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• W2316997685 startingPage "2030" @default.
• W2316997685 abstract "The 5′-phosphorylated oligonucleotides (5′-pONs) are currently synthesized using expensive and sensitive modified phosphoramidite reagents. In this work, a simple, cost-effective, efficient, and automatable method is presented, based on the controlled oxidation of the 5′-terminal alcohol followed by a β-elimination reaction. The latter reaction leads to the removal of the terminal 5′-nucleoside and subsequent formation of the 5′-phosphate moiety. Thus, chemical phosphorylation of oligonucleotides (DNA or RNA) is achieved without using modified phosphoramidites." @default.
• W2316997685 created "2016-06-24" @default.
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• W2316997685 date "2012-04-05" @default.
• W2316997685 modified "2023-10-14" @default.
• W2316997685 title "Phosphorylating Reagent-Free Synthesis of 5′-Phosphate Oligonucleotides by Controlled Oxidative Degradation of Their 5′-End" @default.
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• W2316997685 doi "https://doi.org/10.1021/ol300542s" @default.
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• W2316997685 hasPublicationYear "2012" @default.
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