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- W2317018876 abstract "바지락 단백질의 thermolysin 가수분해물로부터 분자량 한계범위가 10,000 da인 한외여과막을 통과한 저분자량의 물질을 Sephadex LH-20 column을 이용한 겔 크로마토그래피에서 ACE에 대하여 저해 활성을 가지는 3개의 획분을 분취하여 SP-Toyopearl 650S column과 SuperQ-Toyopearl 650S column을 이용한 이온 교환 크로마토그래피에 의하여 4개의 활성 획분을 얻었다. 이 중 가장 높은 절해 활성을 나타내는 획분의 아미노산 조성은 alanine, leucine, isoleucine 및 threonine의 함량이 많았고, 활성 발현에 있어 C 말단 아미노산 잔기로서 중요한 역할을 하는 proline의 함량도 <TEX>$3.7%$</TEX>%인 것으로 나타났다. ACE 저해 활성은 <TEX>$IC_{50}$</TEX> 값이 0.748 <TEX>$mu$</TEX>g이었다. The peptides inhibiting angiotensin converting enzyme (ACE) were isolated from the hydrolysate of manila clam (Ruditapes philippinamm) proteins prepared with thermolysin. The thermolysin hydrolysate was pretreated with membrane filter (MW cut-off 10,000) to obtain the peptide fraction with ACE inhibition. The crude peptides were applied to a Sephadex LH-20 column and eluted with <TEX>$30%$</TEX> methanol. The three active fractions (A, B and C) were collected and concentrated, and then applied to a SP-Toyopearl 650S column equilibrated with distilled water and was eluted with a linear gradient of NaCl concentration (0 to 1 M). The four active fractions (A-1, A-2, B-1 and C-1) were collected and concentrated, and then applied to a SuperQ-Toyopearl 650S column equilibrated with distilled water and was eluted with a linear gradient of NaCl concentration (0 to 1 M). The maximum inhibitory activity was observed in the fraction B-1Q showed the IC_{50} values of 0.748 <TEX>$mu$</TEX>g. The abundant amino acids obtained from active fraction B-1Q were leucine, isoleucine, alanine and threonine." @default.
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- W2317018876 date "2002-09-01" @default.
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- W2317018876 title "Characteristics of Angiotensin Converting Enzyme Inhibitory Peptides from Thermolysin Hydrolysate of Manila clam, Ruditapes philippinarum Proteins" @default.
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- W2317018876 doi "https://doi.org/10.5657/kfas.2002.35.5.529" @default.
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