FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317029008> ?p ?o ?g. }

• W2317029008 abstract "Phorbol 12-myristate 13-acetate (PMA) affects a variety of cell processes, including proliferation and differentiation. In most normal cell types, PMA enhances proliferation by a protein kinase C (PKC)- dependent mechanism, while the proliferation of cancer cell lines is inhibited by PMA. PMA has been shown to induce cyclooxygenase-2 (COX-2) production in many cell lines. We examined the effect of PMA on func- tions of induced COX-2 on proliferation of head-and-neck UMSCC-22B cancer cells. PMA was found to in- hibit head-and-neck cancer cell proliferation in a cell viability assay and to induce apoptosis. Abundance of p21 protein and p53 in these cells was increased by PMA treatment. The phorbol ester increased p21 expres- sion in 22B cells through the classical PKC pathway and independently of activation of p53. In addition, PMA increased expression of COX-2 in a time-dependent, PKC-requiring manner. COX-2 siRNA increased abundance of both p53 and p21 in 22B cells and importantly potentiated the anti-proliferative effect of PMA. Confocal microscopy revealed that PMA increased nuclear accumulation of COX-2, but not COX-1, and that COX-2 formed complexes with p53, p21 and the coactivator, p300. Chromatin immunoprecipitation showed that nuclear fraction COX-2 and p53 bound to the promoter region of p21. PMA did not significantly increase PGE 2 levels in cultured medium of 22B cells, but did increase by 13-fold the levels of PGE 2 in the medium of immortalized normal (293T) cells. In conclusion, our results show that PMA inhibits cell proliferation in head-and-neck cancer cells by a mechanism that is potentiated by COX-2 siRNA. The latter results in an inc- rease of p53 and p21 in PMA-treated cancer cells. In the absence of its siRNA, COX-2 is increased in PMA- exposed cancer cells and becomes a nucleoprotein, where it complexes with p53 and p21. Thus, the anti- proliferative activity of PMA in 22B cells is moderated by concurrent induction by PMA of COX-2 accumu- lation; this moderation may be due to reduction by COX-2 of availability of p21 and p53 in the nucleus." @default.
• W2317029008 created "2016-06-24" @default.
• W2317029008 creator A5019179538 @default.
• W2317029008 creator A5058165905 @default.
• W2317029008 creator A5059970548 @default.
• W2317029008 creator A5067497938 @default.
• W2317029008 creator A5079132263 @default.
• W2317029008 creator A5088286120 @default.
• W2317029008 date "2009-12-01" @default.
• W2317029008 modified "2023-10-06" @default.
• W2317029008 title "COX-2, Cell Proliferation and PMA in Head-and-Neck Cancer Cells" @default.
• W2317029008 cites W1561747056 @default.
• W2317029008 cites W1746080159 @default.
• W2317029008 cites W1966296779 @default.
• W2317029008 cites W1966677777 @default.
• W2317029008 cites W1968151036 @default.
• W2317029008 cites W1968379411 @default.
• W2317029008 cites W1970395279 @default.
• W2317029008 cites W1975643940 @default.
• W2317029008 cites W1975702359 @default.
• W2317029008 cites W1978265027 @default.
• W2317029008 cites W1978503888 @default.
• W2317029008 cites W1989693627 @default.
• W2317029008 cites W1993210315 @default.
• W2317029008 cites W1993223905 @default.
• W2317029008 cites W1993282564 @default.
• W2317029008 cites W2001692359 @default.
• W2317029008 cites W2006051384 @default.
• W2317029008 cites W2011698701 @default.
• W2317029008 cites W2012074391 @default.
• W2317029008 cites W2015184268 @default.
• W2317029008 cites W2016473921 @default.
• W2317029008 cites W2016715613 @default.
• W2317029008 cites W2020790250 @default.
• W2317029008 cites W2029369936 @default.
• W2317029008 cites W2030109883 @default.
• W2317029008 cites W2032172560 @default.
• W2317029008 cites W2032672429 @default.
• W2317029008 cites W203366438 @default.
• W2317029008 cites W2034269086 @default.
• W2317029008 cites W2041140906 @default.
• W2317029008 cites W2041384877 @default.
• W2317029008 cites W2050888137 @default.
• W2317029008 cites W2052216708 @default.
• W2317029008 cites W2057167919 @default.
• W2317029008 cites W2068824753 @default.
• W2317029008 cites W2073738021 @default.
• W2317029008 cites W2074168286 @default.
• W2317029008 cites W2077868837 @default.
• W2317029008 cites W2078422697 @default.
• W2317029008 cites W2080014451 @default.
• W2317029008 cites W2080532845 @default.
• W2317029008 cites W2082001748 @default.
• W2317029008 cites W2084910239 @default.
• W2317029008 cites W2087908472 @default.
• W2317029008 cites W2091487108 @default.
• W2317029008 cites W2093186323 @default.
• W2317029008 cites W2108193919 @default.
• W2317029008 cites W2110703427 @default.
• W2317029008 cites W2113408403 @default.
• W2317029008 cites W2114304073 @default.
• W2317029008 cites W2116168419 @default.
• W2317029008 cites W2124374523 @default.
• W2317029008 cites W2126853519 @default.
• W2317029008 cites W2153819797 @default.
• W2317029008 cites W2157921426 @default.
• W2317029008 cites W2159973565 @default.
• W2317029008 cites W2162517111 @default.
• W2317029008 cites W2167741764 @default.
• W2317029008 cites W2186980858 @default.
• W2317029008 cites W2313962926 @default.
• W2317029008 cites W2407224227 @default.
• W2317029008 cites W2800526221 @default.
• W2317029008 doi "https://doi.org/10.2174/187152209790773002" @default.
• W2317029008 hasPublicationYear "2009" @default.
• W2317029008 type Work @default.
• W2317029008 sameAs 2317029008 @default.
• W2317029008 citedByCount "2" @default.
• W2317029008 countsByYear W23170290082012 @default.
• W2317029008 countsByYear W23170290082013 @default.
• W2317029008 crossrefType "journal-article" @default.
• W2317029008 hasAuthorship W2317029008A5019179538 @default.
• W2317029008 hasAuthorship W2317029008A5058165905 @default.
• W2317029008 hasAuthorship W2317029008A5059970548 @default.
• W2317029008 hasAuthorship W2317029008A5067497938 @default.
• W2317029008 hasAuthorship W2317029008A5079132263 @default.
• W2317029008 hasAuthorship W2317029008A5088286120 @default.
• W2317029008 hasConcept C121608353 @default.
• W2317029008 hasConcept C1491633281 @default.
• W2317029008 hasConcept C153911025 @default.
• W2317029008 hasConcept C190283241 @default.
• W2317029008 hasConcept C195794163 @default.
• W2317029008 hasConcept C2780043322 @default.
• W2317029008 hasConcept C502942594 @default.
• W2317029008 hasConcept C54355233 @default.
• W2317029008 hasConcept C55493867 @default.
• W2317029008 hasConcept C62112901 @default.
• W2317029008 hasConcept C62478195 @default.
• W2317029008 hasConcept C81885089 @default.
• W2317029008 hasConcept C86803240 @default.

• next