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- W2317110997 abstract "A variant strain of Japanese encephalitis virus (Hotta strain) isolated in adult mice from a patient was obtained by the high concentration passage and by the method of passage at limmiting dilution in hamster kidney cell cultures. The variant strain showed a marked loss in virulence upon intracerebral inoculation into adult mice. The characteristics of the variant strain were as follows:1) The variant strain proved to be avirulent for adult mice when inoculated intracerebrally or subcutaneously but retained virulence for suckling mice inoculated intracerebrally.2) The growth curve revealed that there was no difference in the pattern of virus multiplication in hamster kidney cells between variant and parent strains. However, the rate of multiplication of the variant strain in adult mouse brain was markedly lower than that of parent strain.3) The variant strain after back passages in adult mice, not only failed to increase virulence but also could not multiply in adult mouse brain. This variant strain showed reversion to virulence for adult mice after three back passages in suckling mice." @default.
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- W2317110997 date "1963-01-01" @default.
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- W2317110997 title "STUDIES ON THE VARIATION OF VIRULENCE OF JAPANESE ENCEPHALITIS VIRUS" @default.
- W2317110997 doi "https://doi.org/10.2222/jsv.13.5" @default.
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