Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317118747> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2317118747 endingPage "375" @default.
- W2317118747 startingPage "374" @default.
- W2317118747 abstract "α-Amylases from the intestinal cavity of two tilapia species, Oreochromis niloticus (ONI-AMY) and Sarotherodon melanotheron (SME-AMY), were purified using ammonium sulfate precipitation, affinity chromatography and chromatofocusing procedures. The purification was approximately 100-fold. The amylolytic activity, specific activity, product distribution, pH and temperature profile of ONI-AMY and SME-AMY are quite similar. The molecular mass differs slightly: 56 600 (ONI-AMY) vs. 55 500 (SME-AMY). As shown by isoelectric focusing analysis, both amylases contain two isoforms A and B with distinct pI: 7.2 (A) and 7.8 (B), vs. 8.3 (A) and 8.8 (B), respectively. It was not possible to isolate B, since B converts into A with time. The kinetics of the inhibition of ONI-AMY and SME-AMY activity by α-, β- and γ-cyclodextrin (α-, β- and γ-CD) were investigated using amylose as the substrate. Statistical analysis of the kinetic data expressed using a general velocity equation and assuming rapid equilibrium showed that the inhibition is of the mixed noncompetitive type. Similar results were obtained with ONI-AMY and SME-AMY. β- and γ-CD are stronger inhibitors than α-CD. ONI-AMY and SME-AMY are then closely related and show the general features common to the members of the α-amylase class (family 13). They enable ONI and SME tilapias to digest starch in food." @default.
- W2317118747 created "2016-06-24" @default.
- W2317118747 creator A5045329825 @default.
- W2317118747 date "1981-01-01" @default.
- W2317118747 modified "2023-09-27" @default.
- W2317118747 title "Backyard fish farming" @default.
- W2317118747 doi "https://doi.org/10.1016/0044-8486(81)90073-9" @default.
- W2317118747 hasPublicationYear "1981" @default.
- W2317118747 type Work @default.
- W2317118747 sameAs 2317118747 @default.
- W2317118747 citedByCount "0" @default.
- W2317118747 crossrefType "journal-article" @default.
- W2317118747 hasAuthorship W2317118747A5045329825 @default.
- W2317118747 hasConcept C141896089 @default.
- W2317118747 hasConcept C153911025 @default.
- W2317118747 hasConcept C170835558 @default.
- W2317118747 hasConcept C179247698 @default.
- W2317118747 hasConcept C181199279 @default.
- W2317118747 hasConcept C18903297 @default.
- W2317118747 hasConcept C193363816 @default.
- W2317118747 hasConcept C2776428949 @default.
- W2317118747 hasConcept C2776829962 @default.
- W2317118747 hasConcept C2777289219 @default.
- W2317118747 hasConcept C2779286987 @default.
- W2317118747 hasConcept C2909208804 @default.
- W2317118747 hasConcept C50476208 @default.
- W2317118747 hasConcept C505870484 @default.
- W2317118747 hasConcept C529335014 @default.
- W2317118747 hasConcept C55493867 @default.
- W2317118747 hasConcept C86803240 @default.
- W2317118747 hasConcept C86909935 @default.
- W2317118747 hasConcept C96294017 @default.
- W2317118747 hasConceptScore W2317118747C141896089 @default.
- W2317118747 hasConceptScore W2317118747C153911025 @default.
- W2317118747 hasConceptScore W2317118747C170835558 @default.
- W2317118747 hasConceptScore W2317118747C179247698 @default.
- W2317118747 hasConceptScore W2317118747C181199279 @default.
- W2317118747 hasConceptScore W2317118747C18903297 @default.
- W2317118747 hasConceptScore W2317118747C193363816 @default.
- W2317118747 hasConceptScore W2317118747C2776428949 @default.
- W2317118747 hasConceptScore W2317118747C2776829962 @default.
- W2317118747 hasConceptScore W2317118747C2777289219 @default.
- W2317118747 hasConceptScore W2317118747C2779286987 @default.
- W2317118747 hasConceptScore W2317118747C2909208804 @default.
- W2317118747 hasConceptScore W2317118747C50476208 @default.
- W2317118747 hasConceptScore W2317118747C505870484 @default.
- W2317118747 hasConceptScore W2317118747C529335014 @default.
- W2317118747 hasConceptScore W2317118747C55493867 @default.
- W2317118747 hasConceptScore W2317118747C86803240 @default.
- W2317118747 hasConceptScore W2317118747C86909935 @default.
- W2317118747 hasConceptScore W2317118747C96294017 @default.
- W2317118747 hasLocation W23171187471 @default.
- W2317118747 hasOpenAccess W2317118747 @default.
- W2317118747 hasPrimaryLocation W23171187471 @default.
- W2317118747 hasRelatedWork W1536678566 @default.
- W2317118747 hasRelatedWork W1575207367 @default.
- W2317118747 hasRelatedWork W1993553745 @default.
- W2317118747 hasRelatedWork W2001762240 @default.
- W2317118747 hasRelatedWork W2004124939 @default.
- W2317118747 hasRelatedWork W2021488027 @default.
- W2317118747 hasRelatedWork W2027240780 @default.
- W2317118747 hasRelatedWork W2039260028 @default.
- W2317118747 hasRelatedWork W2125667499 @default.
- W2317118747 hasRelatedWork W2156002144 @default.
- W2317118747 hasVolume "24" @default.
- W2317118747 isParatext "false" @default.
- W2317118747 isRetracted "false" @default.
- W2317118747 magId "2317118747" @default.
- W2317118747 workType "article" @default.