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- W2317128872 abstract "Several difficulties have been encountered in the cultivation of human tissue in vitro.In the first place human fibrin is readily liquefied by fresh tissue, so that when human plasma is used as a culture medium the cells find no framework on which to grow. Losee and Ebeling overcame this difficulty by transferring the tissue fragments at frequent intervals before liquefaction took place. We have solved the problem in another way which does not necessitate frequent transfers. The method consists in using as a culture medium chick plasma, the fibrin of which resists digestion, with the addition of an equal quantity or more of human serum. In this medium the cells grow much more actively than in pure chick plasma. Since there is no liquefaction it is not necessary to make subcultures oftener than every 5 to 7 days.That fresh human tissue cannot always be obtained when wanted has appeared to be another difficulty in the study of human tissues in cultures. We have found, however, that human tissues, just as th..." @default.
- W2317128872 created "2016-06-24" @default.
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- W2317128872 date "1916-03-01" @default.
- W2317128872 modified "2023-09-26" @default.
- W2317128872 title "Technique of cultivating human tissues in vitro" @default.
- W2317128872 doi "https://doi.org/10.3181/00379727-13-56" @default.
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