FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317291711> ?p ?o ?g. }

• W2317291711 endingPage "100" @default.
• W2317291711 startingPage "100" @default.
• W2317291711 abstract "Clostridium botulinum exoenzyme C3 is the prototype of C3-like ADP-ribosyltransferases that modify the GTPases RhoA, B, and C. C3 catalyzes the transfer of an ADP-ribose moiety from the co-substrate nicotinamide adenine dinucleotide (NAD) to asparagine-41 of Rho-GTPases. Although C3 does not possess cell-binding/-translocation domains, C3 is able to efficiently enter intact cells, including neuronal and macrophage-like cells. Conventionally, the detection of C3 uptake into cells is carried out via the gel-shift assay of modified RhoA. Since this gel-shift assay does not always provide clear, evaluable results an additional method to confirm the ADP-ribosylation of RhoA is necessary. Therefore, a new monoclonal antibody has been generated that specifically detects ADP-ribosylated RhoA/B, but not RhoC, in Western blot and immunohistochemical assay. The scFv antibody fragment was selected by phage display using the human naive antibody gene libraries HAL9/10. Subsequently, the antibody was produced as scFv-Fc and was found to be as sensitive as a commercially available RhoA antibody providing reproducible and specific results. We demonstrate that this specific antibody can be successfully applied for the analysis of ADP-ribosylated RhoA/B in C3-treated Chinese hamster ovary (CHO) and HT22 cells. Moreover, ADP-ribosylation of RhoA was detected within 10 min in C3-treated CHO wild-type cells, indicative of C3 cell entry." @default.
• W2317291711 created "2016-06-24" @default.
• W2317291711 creator A5005331384 @default.
• W2317291711 creator A5009314747 @default.
• W2317291711 creator A5014126648 @default.
• W2317291711 creator A5014398419 @default.
• W2317291711 creator A5038812871 @default.
• W2317291711 creator A5044936106 @default.
• W2317291711 creator A5060377377 @default.
• W2317291711 creator A5066473730 @default.
• W2317291711 creator A5090846828 @default.
• W2317291711 date "2016-04-01" @default.
• W2317291711 modified "2023-09-30" @default.
• W2317291711 title "Detection and Quantification of ADP-Ribosylated RhoA/B by Monoclonal Antibody" @default.
• W2317291711 cites W1228353120 @default.
• W2317291711 cites W14741340 @default.
• W2317291711 cites W1513979636 @default.
• W2317291711 cites W1538428447 @default.
• W2317291711 cites W1564404844 @default.
• W2317291711 cites W1592783888 @default.
• W2317291711 cites W1969550600 @default.
• W2317291711 cites W1973653146 @default.
• W2317291711 cites W1977287101 @default.
• W2317291711 cites W1985654711 @default.
• W2317291711 cites W1991542900 @default.
• W2317291711 cites W1993933934 @default.
• W2317291711 cites W1999930864 @default.
• W2317291711 cites W2002010372 @default.
• W2317291711 cites W2007596877 @default.
• W2317291711 cites W2022185453 @default.
• W2317291711 cites W2023022330 @default.
• W2317291711 cites W2023561573 @default.
• W2317291711 cites W2026862338 @default.
• W2317291711 cites W2028140515 @default.
• W2317291711 cites W2030374826 @default.
• W2317291711 cites W2035184466 @default.
• W2317291711 cites W2036145275 @default.
• W2317291711 cites W2041797751 @default.
• W2317291711 cites W2078794156 @default.
• W2317291711 cites W2087583564 @default.
• W2317291711 cites W2100473445 @default.
• W2317291711 cites W2106481926 @default.
• W2317291711 cites W2123129865 @default.
• W2317291711 cites W2124559972 @default.
• W2317291711 cites W2143346248 @default.
• W2317291711 cites W2147138972 @default.
• W2317291711 cites W2176511881 @default.
• W2317291711 cites W2275931451 @default.
• W2317291711 cites W2465096192 @default.
• W2317291711 cites W2749679400 @default.
• W2317291711 cites W4237688674 @default.
• W2317291711 doi "https://doi.org/10.3390/toxins8040100" @default.
• W2317291711 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4848626" @default.
• W2317291711 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27043630" @default.
• W2317291711 hasPublicationYear "2016" @default.
• W2317291711 type Work @default.
• W2317291711 sameAs 2317291711 @default.
• W2317291711 citedByCount "9" @default.
• W2317291711 countsByYear W23172917112016 @default.
• W2317291711 countsByYear W23172917112017 @default.
• W2317291711 countsByYear W23172917112018 @default.
• W2317291711 countsByYear W23172917112021 @default.
• W2317291711 countsByYear W23172917112023 @default.
• W2317291711 crossrefType "journal-article" @default.
• W2317291711 hasAuthorship W2317291711A5005331384 @default.
• W2317291711 hasAuthorship W2317291711A5009314747 @default.
• W2317291711 hasAuthorship W2317291711A5014126648 @default.
• W2317291711 hasAuthorship W2317291711A5014398419 @default.
• W2317291711 hasAuthorship W2317291711A5038812871 @default.
• W2317291711 hasAuthorship W2317291711A5044936106 @default.
• W2317291711 hasAuthorship W2317291711A5060377377 @default.
• W2317291711 hasAuthorship W2317291711A5066473730 @default.
• W2317291711 hasAuthorship W2317291711A5090846828 @default.
• W2317291711 hasBestOaLocation W23172917111 @default.
• W2317291711 hasConcept C104317684 @default.
• W2317291711 hasConcept C153911025 @default.
• W2317291711 hasConcept C159654299 @default.
• W2317291711 hasConcept C170493617 @default.
• W2317291711 hasConcept C175656101 @default.
• W2317291711 hasConcept C185592680 @default.
• W2317291711 hasConcept C203014093 @default.
• W2317291711 hasConcept C2777093181 @default.
• W2317291711 hasConcept C54009773 @default.
• W2317291711 hasConcept C542903549 @default.
• W2317291711 hasConcept C55493867 @default.
• W2317291711 hasConcept C62478195 @default.
• W2317291711 hasConcept C86803240 @default.
• W2317291711 hasConceptScore W2317291711C104317684 @default.
• W2317291711 hasConceptScore W2317291711C153911025 @default.
• W2317291711 hasConceptScore W2317291711C159654299 @default.
• W2317291711 hasConceptScore W2317291711C170493617 @default.
• W2317291711 hasConceptScore W2317291711C175656101 @default.
• W2317291711 hasConceptScore W2317291711C185592680 @default.
• W2317291711 hasConceptScore W2317291711C203014093 @default.
• W2317291711 hasConceptScore W2317291711C2777093181 @default.
• W2317291711 hasConceptScore W2317291711C54009773 @default.
• W2317291711 hasConceptScore W2317291711C542903549 @default.
• W2317291711 hasConceptScore W2317291711C55493867 @default.

• next