FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317361958> ?p ?o ?g. }

• W2317361958 endingPage "9900" @default.
• W2317361958 startingPage "9886" @default.
• W2317361958 abstract "Alanyl-tRNA synthetase, a dimeric class 2 aminoacyl-tRNA synthetase, activates glycine and serine at significant rates. An editing activity hydrolyzes Gly-tRNAala and Ser-tRNAala to ensure fidelity of aminoacylation. Analytical ultracentrifugation demonstrates that the enzyme is predominately a dimer in solution. ATP binding to full length enzyme (ARS875) and to an N-terminal construct (ARS461) is endothermic (ΔH = 3–4 kcal mol–1) with stoichiometries of 1:1 for ARS461 and 2:1 for full-length dimer. Binding of aminoacyl-adenylate analogues, 5′-O-[N-(l-alanyl)sulfamoyl]adenosine (ASAd) and 5′-O-[N-(l-glycinyl)sulfamoyl]adenosine (GSAd), are exothermic; ASAd exhibits a large negative heat capacity change (ΔCp = 0.48 kcal mol–1 K–1). Modification of alanyl-tRNA synthetase with periodate-oxidized tRNAala (otRNAala) generates multiple, covalent, enzyme–tRNAala products. The distribution of these products is altered by ATP, ATP and alanine, and aminoacyl-adenylate analogues (ASAd and GSAd). Alanyl-tRNA synthetase was modified with otRNAala, and tRNA-peptides from tryptic digests were purified by ion exchange chromatography. Six peptides linked through a cyclic dehydromoropholino structure at the 3′-end of tRNAala were sequenced by mass spectrometry. One site lies in the N-terminal adenylate synthesis domain (residue 74), two lie in the opening to the editing site (residues 526 and 585), and three (residues 637, 639, and 648) lie on the back side of the editing domain. At least one additional modification site was inferred from analysis of modification of ARS461. The location of the sites modified by otRNAala suggests that there are multiple modes of interaction of tRNAala with the enzyme, whose distribution is influenced by occupation of the ATP binding site." @default.
• W2317361958 created "2016-06-24" @default.
• W2317361958 creator A5008393401 @default.
• W2317361958 creator A5038648913 @default.
• W2317361958 creator A5044525805 @default.
• W2317361958 creator A5072406189 @default.
• W2317361958 creator A5077675700 @default.
• W2317361958 creator A5082747725 @default.
• W2317361958 date "2011-10-19" @default.
• W2317361958 modified "2023-10-16" @default.
• W2317361958 title "Allosteric Interaction of Nucleotides and tRNA^ala with <i>E. coli</i> Alanyl-tRNA Synthetase" @default.
• W2317361958 cites W1480195325 @default.
• W2317361958 cites W150168246 @default.
• W2317361958 cites W1516634087 @default.
• W2317361958 cites W1516952587 @default.
• W2317361958 cites W1547783763 @default.
• W2317361958 cites W1548292434 @default.
• W2317361958 cites W1572643844 @default.
• W2317361958 cites W1573963187 @default.
• W2317361958 cites W1670590392 @default.
• W2317361958 cites W1965127482 @default.
• W2317361958 cites W1965790693 @default.
• W2317361958 cites W1968202297 @default.
• W2317361958 cites W1969211306 @default.
• W2317361958 cites W1971128922 @default.
• W2317361958 cites W1971604056 @default.
• W2317361958 cites W1973010540 @default.
• W2317361958 cites W1974996001 @default.
• W2317361958 cites W1975317507 @default.
• W2317361958 cites W1976949015 @default.
• W2317361958 cites W1980389783 @default.
• W2317361958 cites W1981529363 @default.
• W2317361958 cites W1983263986 @default.
• W2317361958 cites W1983918957 @default.
• W2317361958 cites W1984486881 @default.
• W2317361958 cites W1987446193 @default.
• W2317361958 cites W1989955819 @default.
• W2317361958 cites W1991324280 @default.
• W2317361958 cites W1995833227 @default.
• W2317361958 cites W1999689180 @default.
• W2317361958 cites W2000672590 @default.
• W2317361958 cites W2001013848 @default.
• W2317361958 cites W2001091996 @default.
• W2317361958 cites W2003466068 @default.
• W2317361958 cites W2003646873 @default.
• W2317361958 cites W2004557261 @default.
• W2317361958 cites W2005392755 @default.
• W2317361958 cites W2007239250 @default.
• W2317361958 cites W2012804004 @default.
• W2317361958 cites W2013165826 @default.
• W2317361958 cites W2015796466 @default.
• W2317361958 cites W2016583173 @default.
• W2317361958 cites W2017337363 @default.
• W2317361958 cites W2020961118 @default.
• W2317361958 cites W2021173027 @default.
• W2317361958 cites W2023909746 @default.
• W2317361958 cites W2026836226 @default.
• W2317361958 cites W2027707902 @default.
• W2317361958 cites W2028091995 @default.
• W2317361958 cites W2030616609 @default.
• W2317361958 cites W2032927420 @default.
• W2317361958 cites W2040544046 @default.
• W2317361958 cites W2047518536 @default.
• W2317361958 cites W2047792576 @default.
• W2317361958 cites W2051065954 @default.
• W2317361958 cites W2055679353 @default.
• W2317361958 cites W2063309485 @default.
• W2317361958 cites W2065178804 @default.
• W2317361958 cites W2065873716 @default.
• W2317361958 cites W2069469166 @default.
• W2317361958 cites W2071375060 @default.
• W2317361958 cites W2074077766 @default.
• W2317361958 cites W2077171101 @default.
• W2317361958 cites W2077855194 @default.
• W2317361958 cites W2082135034 @default.
• W2317361958 cites W2083731728 @default.
• W2317361958 cites W2084692577 @default.
• W2317361958 cites W2086218516 @default.
• W2317361958 cites W2089119659 @default.
• W2317361958 cites W2089143068 @default.
• W2317361958 cites W2089433747 @default.
• W2317361958 cites W2091313066 @default.
• W2317361958 cites W2095421158 @default.
• W2317361958 cites W2095554932 @default.
• W2317361958 cites W2100837269 @default.
• W2317361958 cites W2110914875 @default.
• W2317361958 cites W2111228714 @default.
• W2317361958 cites W2114972602 @default.
• W2317361958 cites W2120014697 @default.
• W2317361958 cites W2121750164 @default.
• W2317361958 cites W2140885472 @default.
• W2317361958 cites W2151380530 @default.
• W2317361958 cites W2155283071 @default.
• W2317361958 cites W2165397816 @default.
• W2317361958 cites W2165765949 @default.
• W2317361958 cites W2168048325 @default.
• W2317361958 cites W3022518105 @default.
• W2317361958 cites W3140762575 @default.

• next