FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317363774> ?p ?o ?g. }

• W2317363774 endingPage "303" @default.
• W2317363774 startingPage "297" @default.
• W2317363774 abstract "Fate mapping studies have revealed that bone morphogenetic protein 4 (BMP4) signaling has a key role in segregation of primordial germ cells from proximal epiblast. Adding BMP4 to the culture media of embryonic stem (ES) cells could induce expression of germ cell markers; however, to provide a desired number of germ cells has remained a challenge. In the current study, we intended to establish an in vitro system to obtain reliable germ cells derived from ES cells. Differentiation was induced in ES cells via embryoid body (EB) and monolayer culture system. Cells were cultured with BMP4 from the beginning (++BMP4) or after 48 hours (+BMP4) of culturing for five days. The cultures were assessed for alkaline phosphatase (ALP) activity, expression of Oct4, Mvh and c-kit. In EB culture protocol, the expression of Mvh, Oct4 and ALP activity significantly increased in +BMP4 culture condition, but a significant down-regulation in the expression of germ cell markers was shown in ++BMP4 condition compared with the control group. Parallel differentiation experiments using monolayer culture system indicated the number of putative germ cells did not change. In the current study, we compared two differentiation methods (EB and monolayer) to achieve an optimal germ cell production. The EBs with a short exposure time period to BMP4, showing typical characteristics of germ cells. Therefore, our approach provides a strategy for the production of germline cells from ES cells." @default.
• W2317363774 created "2016-06-24" @default.
• W2317363774 creator A5003987201 @default.
• W2317363774 creator A5010740857 @default.
• W2317363774 creator A5037182687 @default.
• W2317363774 creator A5047525370 @default.
• W2317363774 creator A5071507354 @default.
• W2317363774 creator A5090006467 @default.
• W2317363774 date "2014-01-01" @default.
• W2317363774 modified "2023-09-23" @default.
• W2317363774 title "Delayed BMP4 exposure increases germ cell differentiation in mouse embryonic stem cells." @default.
• W2317363774 cites W1480800427 @default.
• W2317363774 cites W1966697925 @default.
• W2317363774 cites W1984739330 @default.
• W2317363774 cites W1997444723 @default.
• W2317363774 cites W2011458783 @default.
• W2317363774 cites W2018006224 @default.
• W2317363774 cites W2027267818 @default.
• W2317363774 cites W2035566476 @default.
• W2317363774 cites W2036105826 @default.
• W2317363774 cites W2048107295 @default.
• W2317363774 cites W2051952924 @default.
• W2317363774 cites W2056089399 @default.
• W2317363774 cites W2057336811 @default.
• W2317363774 cites W2063754785 @default.
• W2317363774 cites W2068655353 @default.
• W2317363774 cites W2087769305 @default.
• W2317363774 cites W2090663394 @default.
• W2317363774 cites W2092966160 @default.
• W2317363774 cites W2095918275 @default.
• W2317363774 cites W2100705570 @default.
• W2317363774 cites W2107808406 @default.
• W2317363774 cites W2109546355 @default.
• W2317363774 cites W2132608925 @default.
• W2317363774 cites W2143023822 @default.
• W2317363774 cites W2145182226 @default.
• W2317363774 cites W2146506158 @default.
• W2317363774 cites W2147394797 @default.
• W2317363774 cites W2157195988 @default.
• W2317363774 cites W2336102072 @default.
• W2317363774 cites W4210272719 @default.
• W2317363774 cites W2091670449 @default.
• W2317363774 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24969978" @default.
• W2317363774 hasPublicationYear "2014" @default.
• W2317363774 type Work @default.
• W2317363774 sameAs 2317363774 @default.
• W2317363774 citedByCount "3" @default.
• W2317363774 countsByYear W23173637742014 @default.
• W2317363774 countsByYear W23173637742017 @default.
• W2317363774 countsByYear W23173637742019 @default.
• W2317363774 crossrefType "journal-article" @default.
• W2317363774 hasAuthorship W2317363774A5003987201 @default.
• W2317363774 hasAuthorship W2317363774A5010740857 @default.
• W2317363774 hasAuthorship W2317363774A5037182687 @default.
• W2317363774 hasAuthorship W2317363774A5047525370 @default.
• W2317363774 hasAuthorship W2317363774A5071507354 @default.
• W2317363774 hasAuthorship W2317363774A5090006467 @default.
• W2317363774 hasConcept C104317684 @default.
• W2317363774 hasConcept C107459253 @default.
• W2317363774 hasConcept C129536746 @default.
• W2317363774 hasConcept C143948831 @default.
• W2317363774 hasConcept C145103041 @default.
• W2317363774 hasConcept C148738053 @default.
• W2317363774 hasConcept C160160445 @default.
• W2317363774 hasConcept C181199279 @default.
• W2317363774 hasConcept C18155467 @default.
• W2317363774 hasConcept C190446060 @default.
• W2317363774 hasConcept C196843134 @default.
• W2317363774 hasConcept C20875687 @default.
• W2317363774 hasConcept C2779122487 @default.
• W2317363774 hasConcept C2781316418 @default.
• W2317363774 hasConcept C28328180 @default.
• W2317363774 hasConcept C54355233 @default.
• W2317363774 hasConcept C55493867 @default.
• W2317363774 hasConcept C75059768 @default.
• W2317363774 hasConcept C81885089 @default.
• W2317363774 hasConcept C86803240 @default.
• W2317363774 hasConcept C87073359 @default.
• W2317363774 hasConcept C95444343 @default.
• W2317363774 hasConceptScore W2317363774C104317684 @default.
• W2317363774 hasConceptScore W2317363774C107459253 @default.
• W2317363774 hasConceptScore W2317363774C129536746 @default.
• W2317363774 hasConceptScore W2317363774C143948831 @default.
• W2317363774 hasConceptScore W2317363774C145103041 @default.
• W2317363774 hasConceptScore W2317363774C148738053 @default.
• W2317363774 hasConceptScore W2317363774C160160445 @default.
• W2317363774 hasConceptScore W2317363774C181199279 @default.
• W2317363774 hasConceptScore W2317363774C18155467 @default.
• W2317363774 hasConceptScore W2317363774C190446060 @default.
• W2317363774 hasConceptScore W2317363774C196843134 @default.
• W2317363774 hasConceptScore W2317363774C20875687 @default.
• W2317363774 hasConceptScore W2317363774C2779122487 @default.
• W2317363774 hasConceptScore W2317363774C2781316418 @default.
• W2317363774 hasConceptScore W2317363774C28328180 @default.
• W2317363774 hasConceptScore W2317363774C54355233 @default.
• W2317363774 hasConceptScore W2317363774C55493867 @default.
• W2317363774 hasConceptScore W2317363774C75059768 @default.
• W2317363774 hasConceptScore W2317363774C81885089 @default.

• next