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- W2317381279 abstract "Anodic aluminum oxide (AAO) is a porous material having aligned cylindrical compartments with 55–60 nm diameter pores, and being several micrometers deep. A protocol was developed to generate pore-spanning fluid lipid bilayers separating the attoliter-sized compartments of the nanoporous material from the bulk solution, while preserving the optical transparency of the AAO. The AAO was selectively functionalized by silane chemistry to spread giant unilamellar vesicles (GUVs) resulting in large continuous membrane patches covering the pores. Formation of fluid single lipid bilayers through GUV rupture could be readily observed by fluorescence microscopy and further supported by conservation of membrane surface area, before and after GUV rupture. Fluorescence recovery after photobleaching gave low immobile fractions (5–15%) and lipid diffusion coefficients similar to those found for bilayers on silica. The entrapment of molecules within the porous underlying cylindrical compartments, as well as the exclusion of macromolecules from the nanopores, demonstrate the barrier function of the pore-spanning membranes and could be investigated in three-dimensions using confocal laser scanning fluorescence imaging." @default.
- W2317381279 created "2016-06-24" @default.
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- W2317381279 creator A5068263651 @default.
- W2317381279 creator A5071355052 @default.
- W2317381279 date "2011-08-02" @default.
- W2317381279 modified "2023-10-16" @default.
- W2317381279 title "Separating Attoliter-Sized Compartments Using Fluid Pore-Spanning Lipid Bilayers" @default.
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- W2317381279 doi "https://doi.org/10.1021/nn201266e" @default.
- W2317381279 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21797231" @default.
- W2317381279 hasPublicationYear "2011" @default.
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