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- W2317382557 abstract "Abstract Tracer experiments were carried out with anthers of Tulipa cv. Apeldoorn and Cucurbita maxima. Labelled sporopollenin fractions were enriched either by a conventional harsh or by a gentle method. In each case phenylalanine was incorporated at higher rates into the sporopollenin fraction of Tulipa than any of the other precursors used. The high incorporation of phenylalanine was observed at several stages of the postmeiotic development of Tulipa anthers. Even higher incorporation rates were obtained using Cucurbita anthers. The incorporation of [U-l4C]-phenylalanine, [ring U-14C]-phenylalanine and [l-14C]-phenylaIanine into the sporopollenin of Tulipa was compared. The results indicate, that the whole carbon skeleton of phenylalanine is incorporated, pointing to an involvement of the phenylpropanoid metabolism. After potash fusion of a sporopollenin fraction labelled via [ring U-14C]-phenylalanine, p-hydroxybenzoic acid was the main labelled product among the ether soluble acids. Incubation of whole anthers with phenylalanine led to a substantially labelled sporopollenin fraction, whereas incubation of whole anthers with acetate yields only unsatisfactory labelling of this fraction. To overcome this problem, incubation of cross sections of Cucurbita anthers proved to be a promising method, as the incorporation rates obtained with this system and method surpassed those gained by any other method." @default.
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- W2317382557 date "1993-01-01" @default.
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- W2317382557 title "Tracer Experiments on Sporopollenin Biosynthesis" @default.
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- W2317382557 doi "https://doi.org/10.1080/00173139309427447" @default.
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