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- W2317386954 abstract "Aims: In this study, an enzymatic zinc determination method was based on the r egaining of the activity of apo carbonic anhydrase by the zinc is presented in the sample. Study Design:This method determines the amount ofzincin the waste waterofthe environment canbe used in orderto find outhow much contaminated.For this purpose, wastewater from seven different areas and three drinking waters in Erzurum were taken and zinc concentrations were determined . Methodology: Carbonic anhydrase was purified by Sepharose-4B-L tyrosine sulphanilamidaffinity chromatography from bovine erythrocytes. The zinc in this structure was removed by dialysis against dipicolinic acid resulting in apo carbonic anhydrase obtained at a ratio of 100%. The activity of the enzyme was determined by the esterase activity on p -nitrophenylacetate. For comparison purposes, the same samples were analyzed in an atomic absorption (AAS). Results: When we compared the results of the AA and the enzymatic method, we Original ResearchArticle" @default.
- W2317386954 created "2016-06-24" @default.
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- W2317386954 date "2014-01-10" @default.
- W2317386954 modified "2023-09-23" @default.
- W2317386954 title "An Enzymatic Method for Zinc Determination in Wastewater" @default.
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- W2317386954 doi "https://doi.org/10.9734/arrb/2014/10701" @default.
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