FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317400971> ?p ?o ?g. }

• W2317400971 endingPage "025003" @default.
• W2317400971 startingPage "025003" @default.
• W2317400971 abstract "Combining patient-specific cells with the appropriate scaffold to create functional kidneys is a promising technology to provide immunocompatible kidneys for the 100,000+ patients on the organ waiting list. For proper recellularization to occur, the scaffold must possess the critical microstructure and an intact vascular network. Detergent perfusion through the vasculature of a kidney is the preferred method of decellularization; however, harsh detergents could be damaging to the microstructure of the renal tissue and may undesirably solubilize the endogenous growth and signaling factors. In this study, automated decellularization of whole porcine kidneys was performed using an improved method that combined physical and chemical steps to efficiently remove cellular materials while producing minimal damage to the collagenous extracellular matrix (ECM). Freezing/thawing, incremental increases in flow rate under constant pressure, applying osmotic shock to the cellular membranes, and low concentrations of the detergent sodium dodecyl sulfate (SDS) were factors used to decrease SDS exposure time during the decellularization process from 36 to 5 h, which preserved the microstructure while still removing 99% of the DNA. The well-preserved glycosaminoglycans (GAGs) and collagen fibers enhanced cell-ECM interactions. Human renal cortical tubular epithelium (RCTE) cells grew more rapidly when cultured on the ECM obtained from the improved decellularization process and also demonstrated more in vivo-like gene expression patterns. The optimized, automated process that resulted from this work is now used routinely in our laboratory to rapidly decellularize porcine kidneys and could be adapted to other large organs (e.g. heart, liver, and lung)." @default.
• W2317400971 created "2016-06-24" @default.
• W2317400971 creator A5002306237 @default.
• W2317400971 creator A5003605425 @default.
• W2317400971 creator A5006307740 @default.
• W2317400971 creator A5009254358 @default.
• W2317400971 creator A5009991971 @default.
• W2317400971 creator A5019972181 @default.
• W2317400971 creator A5048221077 @default.
• W2317400971 creator A5055476014 @default.
• W2317400971 creator A5070893913 @default.
• W2317400971 date "2016-03-10" @default.
• W2317400971 modified "2023-10-16" @default.
• W2317400971 title "Efficient decellularization of whole porcine kidneys improves reseeded cell behavior" @default.
• W2317400971 cites W1125180050 @default.
• W2317400971 cites W1186988661 @default.
• W2317400971 cites W1521269096 @default.
• W2317400971 cites W1533363236 @default.
• W2317400971 cites W1534366208 @default.
• W2317400971 cites W1731768350 @default.
• W2317400971 cites W1815073662 @default.
• W2317400971 cites W1825249397 @default.
• W2317400971 cites W1833135278 @default.
• W2317400971 cites W1966674393 @default.
• W2317400971 cites W1967540288 @default.
• W2317400971 cites W1970421653 @default.
• W2317400971 cites W1974859278 @default.
• W2317400971 cites W1982413446 @default.
• W2317400971 cites W1982792642 @default.
• W2317400971 cites W1988411148 @default.
• W2317400971 cites W1998029614 @default.
• W2317400971 cites W2009315388 @default.
• W2317400971 cites W2010748138 @default.
• W2317400971 cites W2013425122 @default.
• W2317400971 cites W2016605650 @default.
• W2317400971 cites W2018701070 @default.
• W2317400971 cites W2019336268 @default.
• W2317400971 cites W2029706942 @default.
• W2317400971 cites W2031493544 @default.
• W2317400971 cites W2042510811 @default.
• W2317400971 cites W2052627630 @default.
• W2317400971 cites W2059486645 @default.
• W2317400971 cites W2064890385 @default.
• W2317400971 cites W2066436635 @default.
• W2317400971 cites W2068782007 @default.
• W2317400971 cites W2069663752 @default.
• W2317400971 cites W2070038950 @default.
• W2317400971 cites W2074519869 @default.
• W2317400971 cites W2075695335 @default.
• W2317400971 cites W2075943744 @default.
• W2317400971 cites W2082343955 @default.
• W2317400971 cites W2085289337 @default.
• W2317400971 cites W2092490016 @default.
• W2317400971 cites W2098840758 @default.
• W2317400971 cites W2101228997 @default.
• W2317400971 cites W2103720021 @default.
• W2317400971 cites W2107381020 @default.
• W2317400971 cites W2110230080 @default.
• W2317400971 cites W2110549425 @default.
• W2317400971 cites W213050918 @default.
• W2317400971 cites W2138989174 @default.
• W2317400971 cites W2140577676 @default.
• W2317400971 cites W2145468439 @default.
• W2317400971 cites W2150862103 @default.
• W2317400971 cites W2152056221 @default.
• W2317400971 cites W2154802152 @default.
• W2317400971 cites W2161123465 @default.
• W2317400971 cites W2170843321 @default.
• W2317400971 cites W4230240787 @default.
• W2317400971 cites W4239923395 @default.
• W2317400971 doi "https://doi.org/10.1088/1748-6041/11/2/025003" @default.
• W2317400971 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26963774" @default.
• W2317400971 hasPublicationYear "2016" @default.
• W2317400971 type Work @default.
• W2317400971 sameAs 2317400971 @default.
• W2317400971 citedByCount "45" @default.
• W2317400971 countsByYear W23174009712016 @default.
• W2317400971 countsByYear W23174009712017 @default.
• W2317400971 countsByYear W23174009712018 @default.
• W2317400971 countsByYear W23174009712019 @default.
• W2317400971 countsByYear W23174009712020 @default.
• W2317400971 countsByYear W23174009712021 @default.
• W2317400971 countsByYear W23174009712022 @default.
• W2317400971 countsByYear W23174009712023 @default.
• W2317400971 crossrefType "journal-article" @default.
• W2317400971 hasAuthorship W2317400971A5002306237 @default.
• W2317400971 hasAuthorship W2317400971A5003605425 @default.
• W2317400971 hasAuthorship W2317400971A5006307740 @default.
• W2317400971 hasAuthorship W2317400971A5009254358 @default.
• W2317400971 hasAuthorship W2317400971A5009991971 @default.
• W2317400971 hasAuthorship W2317400971A5019972181 @default.
• W2317400971 hasAuthorship W2317400971A5048221077 @default.
• W2317400971 hasAuthorship W2317400971A5055476014 @default.
• W2317400971 hasAuthorship W2317400971A5070893913 @default.
• W2317400971 hasConcept C126322002 @default.
• W2317400971 hasConcept C136229726 @default.
• W2317400971 hasConcept C142724271 @default.
• W2317400971 hasConcept C146957229 @default.

• next