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• W2317451352 endingPage "971" @default.
• W2317451352 startingPage "941" @default.
• W2317451352 abstract "Tolerogenic dendritic cells (DC) that are maturation-resistant and locked in a semimature state are promising tools in clinical applications for tolerance induction. Different immunomodulatory agents have been shown to induce a tolerogenic DC phenotype, such as the biologically active form of vitamin D (1,25(OH)2D3), glucocorticoids, and a synergistic combination of both. In this study, we aimed to characterize the protein profile, function and phenotype of DCs obtained in vitro in the presence of 1,25(OH)2D3, dexamethasone (DEX), and a combination of both compounds (combi). Human CD14+ monocytes were differentiated toward mature DCs, in the presence or absence of 1,25(OH)2D3 and/or DEX. Cells were prefractionated into cytoplasmic and microsomal fractions and protein samples were separated in two different pH ranges (pH 3–7NL and 6–9), analyzed by 2D-DIGE and differentially expressed spots (p < 0.05) were identified after MALDI-TOF/TOF analysis. In parallel, morphological and phenotypical analyses were performed, revealing that 1,25(OH)2D3- and combi-mDCs are closer related to each other than DEX-mDCs. This was translated in their protein profile, indicating that 1,25(OH)2D3 is more potent than DEX in inducing a tolerogenic profile on human DCs. Moreover, we demonstrate that combining 1,25(OH)2D3 with DEX induces a unique protein expression pattern with major imprinting of the 1,25(OH)2D3 effect. Finally, protein interaction networks and pathway analysis suggest that 1,25(OH)2D3, rather than DEX treatment, has a severe impact on metabolic pathways involving lipids, glucose, and oxidative phosphorylation, which may affect the production of or the response to ROS generation. These findings provide new insights on the molecular basis of DC tolerogenicity induced by 1,25(OH)2D3 and/or DEX, which may lead to the discovery of new pathways involved in DC immunomodulation." @default.
• W2317451352 created "2016-06-24" @default.
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• W2317451352 date "2011-12-15" @default.
• W2317451352 modified "2023-09-26" @default.
• W2317451352 title "Differential Protein Pathways in 1,25-Dihydroxyvitamin D₃ and Dexamethasone Modulated Tolerogenic Human Dendritic Cells" @default.
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• W2317451352 cites W1963913202 @default.
• W2317451352 cites W1969832079 @default.
• W2317451352 cites W1971424572 @default.
• W2317451352 cites W1975996892 @default.
• W2317451352 cites W1977804553 @default.
• W2317451352 cites W1982428412 @default.
• W2317451352 cites W1985396486 @default.
• W2317451352 cites W1989090840 @default.
• W2317451352 cites W1993754466 @default.
• W2317451352 cites W1997708871 @default.
• W2317451352 cites W2000323986 @default.
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• W2317451352 cites W2009446037 @default.
• W2317451352 cites W2011923903 @default.
• W2317451352 cites W2027110067 @default.
• W2317451352 cites W2030479446 @default.
• W2317451352 cites W2031536528 @default.
• W2317451352 cites W2037626742 @default.
• W2317451352 cites W2041406972 @default.
• W2317451352 cites W2046831513 @default.
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• W2317451352 cites W2083373216 @default.
• W2317451352 cites W2084422760 @default.
• W2317451352 cites W2086283039 @default.
• W2317451352 cites W2090669345 @default.
• W2317451352 cites W2092242389 @default.
• W2317451352 cites W2096493204 @default.
• W2317451352 cites W2096754987 @default.
• W2317451352 cites W2101781154 @default.
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• W2317451352 cites W2104261859 @default.
• W2317451352 cites W2104464991 @default.
• W2317451352 cites W2117462751 @default.
• W2317451352 cites W2117477752 @default.
• W2317451352 cites W2119984866 @default.
• W2317451352 cites W2124408320 @default.
• W2317451352 cites W2129217532 @default.
• W2317451352 cites W2130687290 @default.
• W2317451352 cites W2132929600 @default.
• W2317451352 cites W2134934993 @default.
• W2317451352 cites W2135674250 @default.
• W2317451352 cites W2137464714 @default.
• W2317451352 cites W2138702511 @default.
• W2317451352 cites W2138961639 @default.
• W2317451352 cites W2139557613 @default.
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• W2317451352 cites W2148861664 @default.
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• W2317451352 cites W2155723007 @default.
• W2317451352 cites W2158714788 @default.
• W2317451352 cites W2165674132 @default.
• W2317451352 cites W2171722021 @default.
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• W2317451352 doi "https://doi.org/10.1021/pr200724e" @default.
• W2317451352 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22103328" @default.
• W2317451352 hasPublicationYear "2011" @default.
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