FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317455709> ?p ?o ?g. }

• W2317455709 endingPage "6796" @default.
• W2317455709 startingPage "6790" @default.
• W2317455709 abstract "Multidrug efflux pumps play important roles in bacteria drug resistance. A major multidrug efflux system in Gram-negative bacteria is composed of the inner membrane transporter AcrB, outer membrane protein channel TolC, and membrane fusion protein AcrA. These three proteins form a large complex that spans both layers of cell membranes and the periplasmic space. AcrB exists and functions as a homotrimer. To identify residues at the trimer interface that play important roles in AcrB function, we conducted site directed mutagenesis and discovered a key residue, R780. Although R780K was partially functional, all other R780 mutants tested were completely nonfunctional. Replacement of R780 by other residues disrupted trimer association. However, a decrease of trimer stability was not the lone cause for the observed loss of activity, because the activity loss could not be restored by strengthening trimer interaction. Using both heat and chemical denaturation methods, we found that the mutation decreased protein stability. Finally, we identified a repressor mutation, M774K, through random mutagenesis. It restored the activity of AcrBR780A to a level close to that of the wild-type protein. To examine the mechanism of activity restoration, we monitored denaturation of AcrBR780A/M774K and found that the repressor mutation improved protein stability. These results suggest that R780 is critical for AcrB stability. When R780 was replaced by Ala, the protein retained the overall structure, still trimerized in the cell membrane, and interacted with AcrA. However, local structural rearrangement might have occurred and lead to the decrease of protein stability and loss of substrate efflux activity." @default.
• W2317455709 created "2016-06-24" @default.
• W2317455709 creator A5007950824 @default.
• W2317455709 creator A5009578270 @default.
• W2317455709 creator A5031469247 @default.
• W2317455709 creator A5040193425 @default.
• W2317455709 creator A5042177993 @default.
• W2317455709 creator A5056309003 @default.
• W2317455709 creator A5062699888 @default.
• W2317455709 creator A5073592875 @default.
• W2317455709 date "2013-09-16" @default.
• W2317455709 modified "2023-09-23" @default.
• W2317455709 title "Role of a Conserved Residue R780 in Escherichia coli Multidrug Transporter AcrB" @default.
• W2317455709 cites W124728936 @default.
• W2317455709 cites W1556495968 @default.
• W2317455709 cites W1785018154 @default.
• W2317455709 cites W1948806874 @default.
• W2317455709 cites W1964509770 @default.
• W2317455709 cites W1973714359 @default.
• W2317455709 cites W1979973356 @default.
• W2317455709 cites W1985400607 @default.
• W2317455709 cites W2028787442 @default.
• W2317455709 cites W2035081851 @default.
• W2317455709 cites W2042669181 @default.
• W2317455709 cites W2056659809 @default.
• W2317455709 cites W2068119233 @default.
• W2317455709 cites W2070196678 @default.
• W2317455709 cites W2073629943 @default.
• W2317455709 cites W2079146663 @default.
• W2317455709 cites W2090791841 @default.
• W2317455709 cites W2117867117 @default.
• W2317455709 cites W2121303975 @default.
• W2317455709 cites W2132067013 @default.
• W2317455709 cites W2134483159 @default.
• W2317455709 cites W2138857675 @default.
• W2317455709 cites W2142318169 @default.
• W2317455709 cites W2149485676 @default.
• W2317455709 cites W2149849374 @default.
• W2317455709 cites W2150093310 @default.
• W2317455709 cites W2153601116 @default.
• W2317455709 cites W2170213289 @default.
• W2317455709 cites W2170503951 @default.
• W2317455709 doi "https://doi.org/10.1021/bi400452v" @default.
• W2317455709 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24007302" @default.
• W2317455709 hasPublicationYear "2013" @default.
• W2317455709 type Work @default.
• W2317455709 sameAs 2317455709 @default.
• W2317455709 citedByCount "11" @default.
• W2317455709 countsByYear W23174557092014 @default.
• W2317455709 countsByYear W23174557092016 @default.
• W2317455709 countsByYear W23174557092017 @default.
• W2317455709 countsByYear W23174557092018 @default.
• W2317455709 countsByYear W23174557092019 @default.
• W2317455709 countsByYear W23174557092021 @default.
• W2317455709 countsByYear W23174557092022 @default.
• W2317455709 crossrefType "journal-article" @default.
• W2317455709 hasAuthorship W2317455709A5007950824 @default.
• W2317455709 hasAuthorship W2317455709A5009578270 @default.
• W2317455709 hasAuthorship W2317455709A5031469247 @default.
• W2317455709 hasAuthorship W2317455709A5040193425 @default.
• W2317455709 hasAuthorship W2317455709A5042177993 @default.
• W2317455709 hasAuthorship W2317455709A5056309003 @default.
• W2317455709 hasAuthorship W2317455709A5062699888 @default.
• W2317455709 hasAuthorship W2317455709A5073592875 @default.
• W2317455709 hasConcept C104317684 @default.
• W2317455709 hasConcept C115335371 @default.
• W2317455709 hasConcept C143065580 @default.
• W2317455709 hasConcept C144647389 @default.
• W2317455709 hasConcept C146587185 @default.
• W2317455709 hasConcept C149011108 @default.
• W2317455709 hasConcept C16318435 @default.
• W2317455709 hasConcept C178790620 @default.
• W2317455709 hasConcept C185592680 @default.
• W2317455709 hasConcept C200082930 @default.
• W2317455709 hasConcept C201663137 @default.
• W2317455709 hasConcept C2776403692 @default.
• W2317455709 hasConcept C2779546866 @default.
• W2317455709 hasConcept C2910769707 @default.
• W2317455709 hasConcept C41625074 @default.
• W2317455709 hasConcept C44312359 @default.
• W2317455709 hasConcept C46371251 @default.
• W2317455709 hasConcept C47701112 @default.
• W2317455709 hasConcept C547475151 @default.
• W2317455709 hasConcept C55493867 @default.
• W2317455709 hasConcept C57581600 @default.
• W2317455709 hasConcept C65871279 @default.
• W2317455709 hasConcept C86803240 @default.
• W2317455709 hasConceptScore W2317455709C104317684 @default.
• W2317455709 hasConceptScore W2317455709C115335371 @default.
• W2317455709 hasConceptScore W2317455709C143065580 @default.
• W2317455709 hasConceptScore W2317455709C144647389 @default.
• W2317455709 hasConceptScore W2317455709C146587185 @default.
• W2317455709 hasConceptScore W2317455709C149011108 @default.
• W2317455709 hasConceptScore W2317455709C16318435 @default.
• W2317455709 hasConceptScore W2317455709C178790620 @default.
• W2317455709 hasConceptScore W2317455709C185592680 @default.
• W2317455709 hasConceptScore W2317455709C200082930 @default.
• W2317455709 hasConceptScore W2317455709C201663137 @default.

• next