FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317503402> ?p ?o ?g. }

• W2317503402 endingPage "7340" @default.
• W2317503402 startingPage "7330" @default.
• W2317503402 abstract "pCRAMOLL 1 is a major, non-glycosylated isolectin found in seeds of Cratylia mollis, which belongs to the Leguminosae family and the Diocleinae subtribe. The lectin (∼25 kDa) consists of 236 amino acids, sharing 82% identity and virtually identical topological architecture with concanavalin A. Both lectins also share the same pH-dependent dimer–tetramer equilibrium and the ability to recognize Glc/Man moieties. Intricate post-translational events occurring in Diocleinae seed cotyledons result in a mixture of intact and fragmented monomers within the oligomeric assemblies of pCRAMOLL 1. In an earlier report, we demonstrated the production, purification, and characterization of the bacterially expressed form of CRAMOLL 1 (rCRAMOLL 1). The recombinant lectin retained sugar-binding activity and several other biophysical properties of pCRAMOLL 1, but its tetramers, which are composed of intact monomers only, show little enhancement in stability when probed with acidification, high temperatures, or hydrostatic pressure. Here we examined the urea-induced unfolding of the nonfragmented tetramers and dimers of rCRAMOLL 1 and compared this behavior with that of the mixed plant lectin counterparts. Using fluorescence, circular dichroism, size-exclusion chromatography, and chemical cross-linking experiments, we posited that the absence of fragmentation lent greater firmness to tetramers, but not to dimers. Dimeric and tetrameric pCRAMOLL 1 unfolded via a compact monomeric intermediate. In contrast, dimers of rCRAMOLL 1 behaved similarly to the plant dimer counterpart, but its tetrameric form remarkably showed no evidence of such partially unfolded monomers. By analyzing the crystal structure of pCRAMOLL 1, we were able to dissect the importance of the fragmentation to lectin stability." @default.
• W2317503402 created "2016-06-24" @default.
• W2317503402 creator A5037403938 @default.
• W2317503402 creator A5055893749 @default.
• W2317503402 creator A5079992522 @default.
• W2317503402 date "2011-08-03" @default.
• W2317503402 modified "2023-10-17" @default.
• W2317503402 title "Characterization of the Unfolding Process of the Tetrameric and Dimeric Forms of <i>Cratylia mollis</i> Seed Lectin (CRAMOLL 1): Effects of Natural Fragmentation on Protein Stability" @default.
• W2317503402 cites W1525383206 @default.
• W2317503402 cites W1538779457 @default.
• W2317503402 cites W1799752396 @default.
• W2317503402 cites W1821925018 @default.
• W2317503402 cites W1964803812 @default.
• W2317503402 cites W1965477136 @default.
• W2317503402 cites W1970660734 @default.
• W2317503402 cites W1979926371 @default.
• W2317503402 cites W1980685592 @default.
• W2317503402 cites W1986699235 @default.
• W2317503402 cites W1995720788 @default.
• W2317503402 cites W1996084104 @default.
• W2317503402 cites W1999119754 @default.
• W2317503402 cites W2010891200 @default.
• W2317503402 cites W2010999015 @default.
• W2317503402 cites W2012072946 @default.
• W2317503402 cites W2013921848 @default.
• W2317503402 cites W2024384673 @default.
• W2317503402 cites W2025672591 @default.
• W2317503402 cites W2026533254 @default.
• W2317503402 cites W2031602260 @default.
• W2317503402 cites W2039054892 @default.
• W2317503402 cites W2047625580 @default.
• W2317503402 cites W2049478023 @default.
• W2317503402 cites W2050335943 @default.
• W2317503402 cites W2056559561 @default.
• W2317503402 cites W2064023816 @default.
• W2317503402 cites W2065153444 @default.
• W2317503402 cites W2068584243 @default.
• W2317503402 cites W2075116156 @default.
• W2317503402 cites W2083129326 @default.
• W2317503402 cites W2085146538 @default.
• W2317503402 cites W2090556967 @default.
• W2317503402 cites W2092584180 @default.
• W2317503402 cites W2095891762 @default.
• W2317503402 cites W2100837269 @default.
• W2317503402 cites W2102135397 @default.
• W2317503402 cites W2108179651 @default.
• W2317503402 cites W2109280121 @default.
• W2317503402 cites W2115752553 @default.
• W2317503402 cites W2119586900 @default.
• W2317503402 cites W2136521767 @default.
• W2317503402 cites W2144263933 @default.
• W2317503402 cites W2170891538 @default.
• W2317503402 cites W3022231175 @default.
• W2317503402 cites W4244736178 @default.
• W2317503402 cites W959845895 @default.
• W2317503402 doi "https://doi.org/10.1021/bi200320x" @default.
• W2317503402 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21790141" @default.
• W2317503402 hasPublicationYear "2011" @default.
• W2317503402 type Work @default.
• W2317503402 sameAs 2317503402 @default.
• W2317503402 citedByCount "19" @default.
• W2317503402 countsByYear W23175034022012 @default.
• W2317503402 countsByYear W23175034022013 @default.
• W2317503402 countsByYear W23175034022014 @default.
• W2317503402 countsByYear W23175034022015 @default.
• W2317503402 countsByYear W23175034022016 @default.
• W2317503402 countsByYear W23175034022017 @default.
• W2317503402 countsByYear W23175034022019 @default.
• W2317503402 countsByYear W23175034022022 @default.
• W2317503402 countsByYear W23175034022023 @default.
• W2317503402 crossrefType "journal-article" @default.
• W2317503402 hasAuthorship W2317503402A5037403938 @default.
• W2317503402 hasAuthorship W2317503402A5055893749 @default.
• W2317503402 hasAuthorship W2317503402A5079992522 @default.
• W2317503402 hasConcept C12554922 @default.
• W2317503402 hasConcept C133571119 @default.
• W2317503402 hasConcept C166940927 @default.
• W2317503402 hasConcept C178790620 @default.
• W2317503402 hasConcept C181199279 @default.
• W2317503402 hasConcept C185592680 @default.
• W2317503402 hasConcept C19869320 @default.
• W2317503402 hasConcept C202751555 @default.
• W2317503402 hasConcept C2778886173 @default.
• W2317503402 hasConcept C2779546866 @default.
• W2317503402 hasConcept C2780758401 @default.
• W2317503402 hasConcept C515207424 @default.
• W2317503402 hasConcept C521977710 @default.
• W2317503402 hasConcept C55493867 @default.
• W2317503402 hasConcept C71240020 @default.
• W2317503402 hasConcept C8010536 @default.
• W2317503402 hasConcept C86803240 @default.
• W2317503402 hasConceptScore W2317503402C12554922 @default.
• W2317503402 hasConceptScore W2317503402C133571119 @default.
• W2317503402 hasConceptScore W2317503402C166940927 @default.
• W2317503402 hasConceptScore W2317503402C178790620 @default.
• W2317503402 hasConceptScore W2317503402C181199279 @default.
• W2317503402 hasConceptScore W2317503402C185592680 @default.
• W2317503402 hasConceptScore W2317503402C19869320 @default.

• next