Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317693875> ?p ?o ?g. }
- W2317693875 endingPage "6" @default.
- W2317693875 startingPage "2" @default.
- W2317693875 abstract "Cell-free protein synthesis (CFPS) systems from crude lysates have benefitted from modifications to their enzyme composition. For example, functionally deleting enzymes in the source strain that are deleterious to CFPS can improve protein synthesis yields. However, making such modifications can take substantial time. As a proof-of-concept to accelerate prototyping capabilities, we assessed the feasibility of using the yeast knockout collection to identify negative effectors in a Saccharomyces cerevisiae CFPS platform. We analyzed extracts made from six deletion strains that targeted the single deletion of potentially negative effectors (e.g., nucleases). We found a statistically significant increase in luciferase yields upon loss of function of GCN3, PEP4, PPT1, NGL3, and XRN1 with a maximum increase of over 6-fold as compared to the wild type. Our work has implications for yeast CFPS and for rapidly prototyping strains to enable cell-free synthetic biology applications." @default.
- W2317693875 created "2016-06-24" @default.
- W2317693875 creator A5025044375 @default.
- W2317693875 creator A5057577685 @default.
- W2317693875 creator A5059800162 @default.
- W2317693875 creator A5069016858 @default.
- W2317693875 creator A5071313722 @default.
- W2317693875 date "2016-03-01" @default.
- W2317693875 modified "2023-10-15" @default.
- W2317693875 title "Yeast knockout library allows for efficient testing of genomic mutations for cell-free protein synthesis" @default.
- W2317693875 cites W1536742797 @default.
- W2317693875 cites W1636733385 @default.
- W2317693875 cites W1776397063 @default.
- W2317693875 cites W1902075119 @default.
- W2317693875 cites W1964253329 @default.
- W2317693875 cites W1972926812 @default.
- W2317693875 cites W1973873896 @default.
- W2317693875 cites W1976865287 @default.
- W2317693875 cites W1985001103 @default.
- W2317693875 cites W1985180922 @default.
- W2317693875 cites W1991017037 @default.
- W2317693875 cites W2008686474 @default.
- W2317693875 cites W2012114611 @default.
- W2317693875 cites W2017643918 @default.
- W2317693875 cites W2019943523 @default.
- W2317693875 cites W2021968892 @default.
- W2317693875 cites W2025608159 @default.
- W2317693875 cites W2034810866 @default.
- W2317693875 cites W2037313242 @default.
- W2317693875 cites W2039552715 @default.
- W2317693875 cites W2040613196 @default.
- W2317693875 cites W2045151072 @default.
- W2317693875 cites W2045435533 @default.
- W2317693875 cites W2049974202 @default.
- W2317693875 cites W2051533377 @default.
- W2317693875 cites W2051777566 @default.
- W2317693875 cites W2052060414 @default.
- W2317693875 cites W2060528969 @default.
- W2317693875 cites W2060665625 @default.
- W2317693875 cites W2064815984 @default.
- W2317693875 cites W2066413363 @default.
- W2317693875 cites W2069942860 @default.
- W2317693875 cites W2072348114 @default.
- W2317693875 cites W2076257276 @default.
- W2317693875 cites W2081192945 @default.
- W2317693875 cites W2082275139 @default.
- W2317693875 cites W2083369386 @default.
- W2317693875 cites W2086939601 @default.
- W2317693875 cites W2093588359 @default.
- W2317693875 cites W2101220802 @default.
- W2317693875 cites W2105689851 @default.
- W2317693875 cites W2107351807 @default.
- W2317693875 cites W2111967267 @default.
- W2317693875 cites W2118568444 @default.
- W2317693875 cites W2133707117 @default.
- W2317693875 cites W2137758137 @default.
- W2317693875 cites W2139398575 @default.
- W2317693875 cites W2140948740 @default.
- W2317693875 cites W2146503498 @default.
- W2317693875 cites W2151148056 @default.
- W2317693875 cites W2152364643 @default.
- W2317693875 cites W2153283265 @default.
- W2317693875 cites W2172282586 @default.
- W2317693875 cites W2327010950 @default.
- W2317693875 cites W2332788291 @default.
- W2317693875 cites W4229516061 @default.
- W2317693875 cites W4236366502 @default.
- W2317693875 cites W4245262121 @default.
- W2317693875 doi "https://doi.org/10.1016/j.synbio.2016.02.004" @default.
- W2317693875 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5640588" @default.
- W2317693875 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/29062921" @default.
- W2317693875 hasPublicationYear "2016" @default.
- W2317693875 type Work @default.
- W2317693875 sameAs 2317693875 @default.
- W2317693875 citedByCount "12" @default.
- W2317693875 countsByYear W23176938752016 @default.
- W2317693875 countsByYear W23176938752017 @default.
- W2317693875 countsByYear W23176938752018 @default.
- W2317693875 countsByYear W23176938752020 @default.
- W2317693875 countsByYear W23176938752021 @default.
- W2317693875 countsByYear W23176938752022 @default.
- W2317693875 countsByYear W23176938752023 @default.
- W2317693875 crossrefType "journal-article" @default.
- W2317693875 hasAuthorship W2317693875A5025044375 @default.
- W2317693875 hasAuthorship W2317693875A5057577685 @default.
- W2317693875 hasAuthorship W2317693875A5059800162 @default.
- W2317693875 hasAuthorship W2317693875A5069016858 @default.
- W2317693875 hasAuthorship W2317693875A5071313722 @default.
- W2317693875 hasBestOaLocation W23176938751 @default.
- W2317693875 hasConcept C104317684 @default.
- W2317693875 hasConcept C13575015 @default.
- W2317693875 hasConcept C14036430 @default.
- W2317693875 hasConcept C147816474 @default.
- W2317693875 hasConcept C167625842 @default.
- W2317693875 hasConcept C181199279 @default.
- W2317693875 hasConcept C191908910 @default.
- W2317693875 hasConcept C2777576037 @default.
- W2317693875 hasConcept C2778665413 @default.