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• W2317750924 abstract "Event Abstract Back to Event Multiple sclerosis mesenchymal stromal cells exhibit a distinct gene expression profile and decreased immunosuppressive properties in relation to healthy control cells Gislane Lelis V. De Oliveira1*, Kelen Cristina R. Malmegrim1, Kalil William A. De Lima1, Daniel G. Ribeiro1, Amanda M. Colombini1, Dimas T. Covas1 and Eduardo A. Donadi1 1 National Institute of Science and Technology in Stem Cells and Cell Therapy, Brazil Mesenchymal stromal cells (MSCs) have immunosuppressive effects and have been used for the treatment of autoimmune diseases. We evaluated the gene expression profiles and the in vitro immunommodulatory properties of MSCs obtained from multiple sclerosis patients and controls. Bone-marrow MSCs were isolated by plastic adherence. Gene expression profile was evaluated by microarrays by using Agilent platform. For coculture assays, CFSE-labeled PBMCs were added to patient or control MSCs, and stimulated with PHA. After five days, T-cell proliferation was assessed by flow citometry. IL-10 and TGF-beta were measured in supernatants by CBA flex. The results were analysed by the Limma-T and Mann-whitney tests. The study was approved by the local Ethics Commitee. Compared to controls, we found 641 differentially expressed genes in patient MSC (P<0.01; fold change>2), comprising 262 repressed genes, including TGFB1 and HGF and, 379 induced, among them, IL10 and IL6. The inhibitory effect of patients MSCs (52.1 + 5.6%) on T-cell proliferation was significantly reduced (P=0.024) compared with controls (69.4 + 7.2%). There were no differences in the percentages of CD4+CD25hiFoxp3+ cells in both MSCs cocultures. IL-10 and TGF-beta secretion were significantly diminished (P<0.05) in supernatants from cocultures with patient MSCs (IL-10: 53.8 + 49.4; TGF-beta: 59.4 + 24.6 pg/mL) compared with controls (IL-10: 109.9 + 64.6; TGF-beta: 226.6 + 153.2 pg/mL). Concluding, patient and control MSCs exhbited distinct transcriptional profiles and patient MSCs secreted low amounts of IL-10 and TGF-beta and exhbited decreased ability to inhibit T lymphocyte proliferation. Keywords: Multiple Sclerosis, multipotent mesenchymal stromal cells, Gene Expression Profiling, immunosuppressive mechanisms, Autoimmune Diseases Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: De Oliveira GV, Malmegrim KR, De Lima KA, Ribeiro DG, Colombini AM, Covas DT and Donadi EA (2013). Multiple sclerosis mesenchymal stromal cells exhibit a distinct gene expression profile and decreased immunosuppressive properties in relation to healthy control cells. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.01115 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 20 Jul 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Gislane Lelis V De Oliveira, National Institute of Science and Technology in Stem Cells and Cell Therapy, Ribeirão Preto, Brazil, gislane.lelis@unesp.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Gislane Lelis V De Oliveira Kelen Cristina R Malmegrim Kalil William A De Lima Daniel G Ribeiro Amanda M Colombini Dimas T Covas Eduardo A Donadi Google Gislane Lelis V De Oliveira Kelen Cristina R Malmegrim Kalil William A De Lima Daniel G Ribeiro Amanda M Colombini Dimas T Covas Eduardo A Donadi Google Scholar Gislane Lelis V De Oliveira Kelen Cristina R Malmegrim Kalil William A De Lima Daniel G Ribeiro Amanda M Colombini Dimas T Covas Eduardo A Donadi PubMed Gislane Lelis V De Oliveira Kelen Cristina R Malmegrim Kalil William A De Lima Daniel G Ribeiro Amanda M Colombini Dimas T Covas Eduardo A Donadi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W2317750924 title "Multiple sclerosis mesenchymal stromal cells exhibit a distinct gene expression profile and decreased immunosuppressive properties in relation to healthy control cells" @default.
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