Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317771775> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2317771775 endingPage "S274" @default.
- W2317771775 startingPage "S274" @default.
- W2317771775 abstract "Airway epithelial progenitor cell injury has been implicated in the pathogenesis of Bronchiolitis Obliterans Syndrome (BOS) in human lung recipients. However, the mechanisms linking severe epithelial injury to allograft rejection remain unclear. In this study we used a conditional mouse model to deplete club cells after left lung transplant and followed acute and chronic rejection by longitudinal MRI, determined fibrotic lesions and epithelial differentiation by immunohistochemistry and flourescent microscopy, and determined host and graft cell contribution to haematopoietic, epithelial, endothelial and stromal cells by flow cytometry. Lungs from triple transgenic (Scgb1a1/DT-A) mice allow doxycycline (dox) control of diphtheria toxin (DT-A) expression in Club Cells. Left lungs from Scgb1a1/DT-A were transplanted into mT transgenic mice. mT trangsgenic mice express a membrane bound tomato red florescence protein, which allows lineage tracing of host cells. Recipients were treated with MR1/anti-CD40L on post-operative day (POD) 0 and CTLA4-Ig on POD 2 to induce allograft acceptance. MRI allowed early detection of inflammatory infiltrates and longitudinal graft assessment before and after club cell depletion. Histo-pathological findings revealed ablation of airway epithelium, loss of epithelial specific protein expression in both proximal and distal airways, peribronchiolar fibrosis, fibroblast calluses obstructing airways and subpleural fibrosis. Flow cytometry of single cell digest of the left lung graft reveal that 70% are host derived and only 30% are actual graft cells. Of the host cells 95% are hematopoietic cells, 2% epithelial cells, 1% endothelial cells, 1% fibroblasts. Of the graft cells 38% are hematopoietic cells, 56% epithelial cells, 1% endothelial cells, 5% are fibroblasts. Epithelial injury is sufficient to induce acute rejection, multiple acute rejections or sustained acute rejections result in BO. MRI can quantify and predict time course and extend of rejection. Lineage tracing experiments reveal that 35% of all hematopoietic cells are tissue resident and that host cells contribute to epithelial, endothelial and stromal cells in the graft. Understanding the source of cell types will enable a better understanding of lung fibrosis after transplant." @default.
- W2317771775 created "2016-06-24" @default.
- W2317771775 creator A5026439638 @default.
- W2317771775 creator A5057850897 @default.
- W2317771775 creator A5065982775 @default.
- W2317771775 date "2015-04-01" @default.
- W2317771775 modified "2023-10-14" @default.
- W2317771775 title "Lineage Tracing of Host and Graft Cells After Lung Transplant and During Club Cell Ablation Induces Allograft Rejection" @default.
- W2317771775 doi "https://doi.org/10.1016/j.healun.2015.01.768" @default.
- W2317771775 hasPublicationYear "2015" @default.
- W2317771775 type Work @default.
- W2317771775 sameAs 2317771775 @default.
- W2317771775 citedByCount "0" @default.
- W2317771775 crossrefType "journal-article" @default.
- W2317771775 hasAuthorship W2317771775A5026439638 @default.
- W2317771775 hasAuthorship W2317771775A5057850897 @default.
- W2317771775 hasAuthorship W2317771775A5065982775 @default.
- W2317771775 hasConcept C10205521 @default.
- W2317771775 hasConcept C126322002 @default.
- W2317771775 hasConcept C142724271 @default.
- W2317771775 hasConcept C203014093 @default.
- W2317771775 hasConcept C2776739411 @default.
- W2317771775 hasConcept C2777714996 @default.
- W2317771775 hasConcept C2780559512 @default.
- W2317771775 hasConcept C2781244666 @default.
- W2317771775 hasConcept C2781448352 @default.
- W2317771775 hasConcept C28328180 @default.
- W2317771775 hasConcept C529295009 @default.
- W2317771775 hasConcept C553184892 @default.
- W2317771775 hasConcept C71924100 @default.
- W2317771775 hasConcept C86803240 @default.
- W2317771775 hasConcept C87568996 @default.
- W2317771775 hasConcept C95444343 @default.
- W2317771775 hasConceptScore W2317771775C10205521 @default.
- W2317771775 hasConceptScore W2317771775C126322002 @default.
- W2317771775 hasConceptScore W2317771775C142724271 @default.
- W2317771775 hasConceptScore W2317771775C203014093 @default.
- W2317771775 hasConceptScore W2317771775C2776739411 @default.
- W2317771775 hasConceptScore W2317771775C2777714996 @default.
- W2317771775 hasConceptScore W2317771775C2780559512 @default.
- W2317771775 hasConceptScore W2317771775C2781244666 @default.
- W2317771775 hasConceptScore W2317771775C2781448352 @default.
- W2317771775 hasConceptScore W2317771775C28328180 @default.
- W2317771775 hasConceptScore W2317771775C529295009 @default.
- W2317771775 hasConceptScore W2317771775C553184892 @default.
- W2317771775 hasConceptScore W2317771775C71924100 @default.
- W2317771775 hasConceptScore W2317771775C86803240 @default.
- W2317771775 hasConceptScore W2317771775C87568996 @default.
- W2317771775 hasConceptScore W2317771775C95444343 @default.
- W2317771775 hasIssue "4" @default.
- W2317771775 hasLocation W23177717751 @default.
- W2317771775 hasOpenAccess W2317771775 @default.
- W2317771775 hasPrimaryLocation W23177717751 @default.
- W2317771775 hasRelatedWork W1651112321 @default.
- W2317771775 hasRelatedWork W1984265631 @default.
- W2317771775 hasRelatedWork W2000399519 @default.
- W2317771775 hasRelatedWork W2054378706 @default.
- W2317771775 hasRelatedWork W2137957356 @default.
- W2317771775 hasRelatedWork W2472837931 @default.
- W2317771775 hasRelatedWork W2522898898 @default.
- W2317771775 hasRelatedWork W3004171792 @default.
- W2317771775 hasRelatedWork W3014857393 @default.
- W2317771775 hasRelatedWork W4235775268 @default.
- W2317771775 hasVolume "34" @default.
- W2317771775 isParatext "false" @default.
- W2317771775 isRetracted "false" @default.
- W2317771775 magId "2317771775" @default.
- W2317771775 workType "article" @default.