Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317786253> ?p ?o ?g. }
- W2317786253 endingPage "1325" @default.
- W2317786253 startingPage "1314" @default.
- W2317786253 abstract "Uterine fibroids (UFs) are benign smooth muscle neoplasms affecting up to 70% of reproductive age women. Treatment of symptomatic UFs places a significant economic burden on the US health-care system. Several specific genetic abnormalities have been described as etiologic factors of UFs, suggesting that a low DNA damage repair capacity may be involved in the formation of UF. In this study, we used human fibroid and adjacent myometrial tissues, as well as an in vitro cell culture model, to evaluate the expression of MutS homolog 2 (MSH2), which encodes a protein belongs to the mismatch repair system. In addition, we deciphered the mechanism by which polycomb repressive complex 2 protein, EZH2, deregulates MSH2 in UFs. The RNA expression analysis demonstrated the deregulation of MSH2 expression in UF tissues in comparison to its adjacent myometrium. Notably, protein levels of MSH2 were upregulated in 90% of fibroid tissues (9 of 10) as compared to matched adjacent myometrial tissues. Human fibroid primary cells treated with 3-deazaneplanocin A (DZNep), chemical inhibitor of EZH2, exhibited a significant increase in MSH2 expression (P < .05). Overexpression of EZH2 using an adenoviral vector approach significantly downregulated the expression of MSH2 (P < .05). Chromatin immunoprecipitation assay demonstrated that enrichment of H3K27me3 in promoter regions of MSH2 was significantly decreased in DZNep-treated fibroid cells as compared to vehicle control. These data suggest that EZH2-H3K27me3 regulatory mechanism dynamically changes the expression levels of DNA mismatch repair gene MSH2, through epigenetic mark H3K27me3. MSH2 may be considered as a marker for early detection of UFs." @default.
- W2317786253 created "2016-06-24" @default.
- W2317786253 creator A5003343672 @default.
- W2317786253 creator A5005306051 @default.
- W2317786253 creator A5018143205 @default.
- W2317786253 creator A5021004025 @default.
- W2317786253 creator A5027563231 @default.
- W2317786253 creator A5036376535 @default.
- W2317786253 creator A5048095058 @default.
- W2317786253 creator A5064122666 @default.
- W2317786253 date "2016-10-01" @default.
- W2317786253 modified "2023-09-30" @default.
- W2317786253 title "Identification of Polycomb Group Protein EZH2-Mediated DNA Mismatch Repair Gene MSH2 in Human Uterine Fibroids" @default.
- W2317786253 cites W1813214544 @default.
- W2317786253 cites W1834897949 @default.
- W2317786253 cites W1869094850 @default.
- W2317786253 cites W1938168107 @default.
- W2317786253 cites W1975930473 @default.
- W2317786253 cites W1976385056 @default.
- W2317786253 cites W1977621555 @default.
- W2317786253 cites W1978582852 @default.
- W2317786253 cites W1980682695 @default.
- W2317786253 cites W1987594837 @default.
- W2317786253 cites W1988525036 @default.
- W2317786253 cites W1988986513 @default.
- W2317786253 cites W1989423211 @default.
- W2317786253 cites W1994987687 @default.
- W2317786253 cites W1994988780 @default.
- W2317786253 cites W1995381569 @default.
- W2317786253 cites W1995464598 @default.
- W2317786253 cites W1995652309 @default.
- W2317786253 cites W1996487270 @default.
- W2317786253 cites W1999358239 @default.
- W2317786253 cites W2000543353 @default.
- W2317786253 cites W2004846981 @default.
- W2317786253 cites W2020230403 @default.
- W2317786253 cites W2020419820 @default.
- W2317786253 cites W2024187290 @default.
- W2317786253 cites W2030246401 @default.
- W2317786253 cites W2032656914 @default.
- W2317786253 cites W2034204671 @default.
- W2317786253 cites W2037914568 @default.
- W2317786253 cites W2041765400 @default.
- W2317786253 cites W2044627216 @default.
- W2317786253 cites W2046379282 @default.
- W2317786253 cites W2050658928 @default.
- W2317786253 cites W2057065967 @default.
- W2317786253 cites W2058010619 @default.
- W2317786253 cites W2058723207 @default.
- W2317786253 cites W2059058989 @default.
- W2317786253 cites W2059960765 @default.
- W2317786253 cites W2062082839 @default.
- W2317786253 cites W2064871244 @default.
- W2317786253 cites W2067698888 @default.
- W2317786253 cites W2068323147 @default.
- W2317786253 cites W2068622682 @default.
- W2317786253 cites W2069715989 @default.
- W2317786253 cites W2069967595 @default.
- W2317786253 cites W2070604281 @default.
- W2317786253 cites W2074832122 @default.
- W2317786253 cites W2077842719 @default.
- W2317786253 cites W2085178889 @default.
- W2317786253 cites W2085183370 @default.
- W2317786253 cites W2085357207 @default.
- W2317786253 cites W2091455517 @default.
- W2317786253 cites W2094051261 @default.
- W2317786253 cites W2095935266 @default.
- W2317786253 cites W2096469282 @default.
- W2317786253 cites W2099869529 @default.
- W2317786253 cites W2100046750 @default.
- W2317786253 cites W2100365251 @default.
- W2317786253 cites W2100543217 @default.
- W2317786253 cites W2101370480 @default.
- W2317786253 cites W2102553239 @default.
- W2317786253 cites W2103226094 @default.
- W2317786253 cites W2113629413 @default.
- W2317786253 cites W2113634596 @default.
- W2317786253 cites W2117381929 @default.
- W2317786253 cites W2118203903 @default.
- W2317786253 cites W2121429001 @default.
- W2317786253 cites W2125496315 @default.
- W2317786253 cites W2126623769 @default.
- W2317786253 cites W2126941107 @default.
- W2317786253 cites W2130383496 @default.
- W2317786253 cites W2131526628 @default.
- W2317786253 cites W2133743991 @default.
- W2317786253 cites W2141070809 @default.
- W2317786253 cites W2142356982 @default.
- W2317786253 cites W2150093422 @default.
- W2317786253 cites W2155990718 @default.
- W2317786253 cites W2157568141 @default.
- W2317786253 cites W2159049759 @default.
- W2317786253 cites W2161537686 @default.
- W2317786253 cites W2171449084 @default.
- W2317786253 cites W2394954899 @default.
- W2317786253 cites W2416479275 @default.
- W2317786253 cites W2426886000 @default.
- W2317786253 cites W2602082991 @default.