Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317787653> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2317787653 abstract "The cancer stem cell theory states that a determinate number of cells within a tumor have unique, stem cell-like features to regenerate the entire tumor mass. However, a cancer stem cell may need the right microenvironment to have its greatest tumor initiation potential. We have documented the presence of two stem cell populations within a tumor to yield consistent and most aggressive recapitulation of tumor initiation in vivo. To isolate cancer stem cells, we used the MMTV-PyMT transgenic model of breast cancer in mice to generate stable cell lines from spontaneous breast tumors. Stable in vitro cultures of FACS analyzed cancer stem cells and tumor-residing mesenchymal stem cells were established and tested for tumor initiating ability using two methods of implantation: subcutaneous (s.c.) injection and cleared mammary fat pad (CMFP) injection. To determine the limit of tumor initiation, a highly malignant cancer stem cell culture (419) was injected s.c. This cell culture produced tumors with 1×10 4 , 1×10 3 , and 1×10 2 cells with 100%, 93%, and 33% tumorigenicity, respectively. In comparison, 100% tumorigenicity was obtained with 1×10 2 419 cells implanted in the CMFP, a significant increase compared to s.c. injection (Chi-squared, p 4 cells but 13% tumorigenicity with 1×10 3 cells. To increase tumorigenicity at low cell numbers, cancer stem cell cultures were combined with tumor-residing mesenchymal stem cells just prior to injection. Cancer stem cell cultures were s.c. injected with 1×10 5 tumor-residing mesenchymal stem cells (383) at the tumor limiting dose, 1×10 1 for 419 and 1×10 3 for 145; tumorigenicity increased 160% in the highly malignant cells (419) and 133% in the less malignant cells (145). The tumor-residing mesenchymal stem cells increased tumorigenicity significantly compared to cancer stem cell injection alone (Chi-squared, p Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 3344." @default.
- W2317787653 created "2016-06-24" @default.
- W2317787653 creator A5011900970 @default.
- W2317787653 creator A5014610282 @default.
- W2317787653 creator A5083792252 @default.
- W2317787653 creator A5090632544 @default.
- W2317787653 date "2010-04-15" @default.
- W2317787653 modified "2023-09-27" @default.
- W2317787653 title "Abstract 3344: Two different stem cell populations exist in breast cancer to control tumor initiation" @default.
- W2317787653 doi "https://doi.org/10.1158/1538-7445.am10-3344" @default.
- W2317787653 hasPublicationYear "2010" @default.
- W2317787653 type Work @default.
- W2317787653 sameAs 2317787653 @default.
- W2317787653 citedByCount "0" @default.
- W2317787653 crossrefType "proceedings-article" @default.
- W2317787653 hasAuthorship W2317787653A5011900970 @default.
- W2317787653 hasAuthorship W2317787653A5014610282 @default.
- W2317787653 hasAuthorship W2317787653A5083792252 @default.
- W2317787653 hasAuthorship W2317787653A5090632544 @default.
- W2317787653 hasConcept C121608353 @default.
- W2317787653 hasConcept C126322002 @default.
- W2317787653 hasConcept C142724271 @default.
- W2317787653 hasConcept C198826908 @default.
- W2317787653 hasConcept C28328180 @default.
- W2317787653 hasConcept C502942594 @default.
- W2317787653 hasConcept C530470458 @default.
- W2317787653 hasConcept C55427017 @default.
- W2317787653 hasConcept C71924100 @default.
- W2317787653 hasConcept C86803240 @default.
- W2317787653 hasConcept C95444343 @default.
- W2317787653 hasConcept C96232424 @default.
- W2317787653 hasConceptScore W2317787653C121608353 @default.
- W2317787653 hasConceptScore W2317787653C126322002 @default.
- W2317787653 hasConceptScore W2317787653C142724271 @default.
- W2317787653 hasConceptScore W2317787653C198826908 @default.
- W2317787653 hasConceptScore W2317787653C28328180 @default.
- W2317787653 hasConceptScore W2317787653C502942594 @default.
- W2317787653 hasConceptScore W2317787653C530470458 @default.
- W2317787653 hasConceptScore W2317787653C55427017 @default.
- W2317787653 hasConceptScore W2317787653C71924100 @default.
- W2317787653 hasConceptScore W2317787653C86803240 @default.
- W2317787653 hasConceptScore W2317787653C95444343 @default.
- W2317787653 hasConceptScore W2317787653C96232424 @default.
- W2317787653 hasLocation W23177876531 @default.
- W2317787653 hasOpenAccess W2317787653 @default.
- W2317787653 hasPrimaryLocation W23177876531 @default.
- W2317787653 hasRelatedWork W1195175053 @default.
- W2317787653 hasRelatedWork W1973607597 @default.
- W2317787653 hasRelatedWork W1982432361 @default.
- W2317787653 hasRelatedWork W1987279711 @default.
- W2317787653 hasRelatedWork W2002818028 @default.
- W2317787653 hasRelatedWork W2012346308 @default.
- W2317787653 hasRelatedWork W2035289469 @default.
- W2317787653 hasRelatedWork W2086828029 @default.
- W2317787653 hasRelatedWork W2133134209 @default.
- W2317787653 hasRelatedWork W2149128188 @default.
- W2317787653 hasRelatedWork W2166342810 @default.
- W2317787653 hasRelatedWork W2171066493 @default.
- W2317787653 hasRelatedWork W2317088387 @default.
- W2317787653 hasRelatedWork W2319606483 @default.
- W2317787653 hasRelatedWork W2320455620 @default.
- W2317787653 hasRelatedWork W2345194485 @default.
- W2317787653 hasRelatedWork W2740527389 @default.
- W2317787653 hasRelatedWork W2745875180 @default.
- W2317787653 hasRelatedWork W3189708764 @default.
- W2317787653 hasRelatedWork W2894843669 @default.
- W2317787653 isParatext "false" @default.
- W2317787653 isRetracted "false" @default.
- W2317787653 magId "2317787653" @default.
- W2317787653 workType "article" @default.