Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317857526> ?p ?o ?g. }
- W2317857526 endingPage "80" @default.
- W2317857526 startingPage "80" @default.
- W2317857526 abstract "The Gram-negative bacterium Gallibacterium anatis is a major cause of salpingitis and peritonitis in commercial egg-layers, leading to reduced egg production and increased mortality. Unfortunately, widespread multidrug resistance and antigenic diversity makes it difficult to control infections and novel prevention strategies are urgently needed. In this study, a pan-genomic reverse vaccinology (RV) approach was used to identify potential vaccine candidates. Firstly, the genomes of 10 selected Gallibacterium strains were analyzed and proteins selected on the following criteria; predicted surface-exposure or secretion, none or one transmembrane helix (TMH), and presence in six or more of the 10 genomes. In total, 42 proteins were selected. The genes encoding 27 of these proteins were successfully cloned in Escherichia coli and the proteins expressed and purified. To reduce the number of vaccine candidates for in vivo testing, each of the purified recombinant proteins was screened by ELISA for their ability to elicit a significant serological response with serum from chickens that had been infected with G. anatis. Additionally, an in silico prediction of the protective potential was carried out based on a protein property prediction method. Of the 27 proteins, two novel putative immunogens were identified; Gab_1309 and Gab_2312. Moreover, three previously characterized virulence factors; GtxA, FlfA and Gab_2156, were identified. Thus, by combining the pan-genomic RV approach with subsequent in vitro and in silico screening, we have narrowed down the pan-proteome of G. anatis to five vaccine candidates. Importantly, preliminary immunization trials indicated an in vivo protective potential of GtxA-N, FlfA and Gab_1309." @default.
- W2317857526 created "2016-06-24" @default.
- W2317857526 creator A5008515030 @default.
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- W2317857526 creator A5088404965 @default.
- W2317857526 date "2014-01-01" @default.
- W2317857526 modified "2023-10-17" @default.
- W2317857526 title "In silico prediction of" @default.
- W2317857526 cites W1214546200 @default.
- W2317857526 cites W1481750421 @default.
- W2317857526 cites W1483692899 @default.
- W2317857526 cites W1494075612 @default.
- W2317857526 cites W1498410508 @default.
- W2317857526 cites W1572643844 @default.
- W2317857526 cites W1574109141 @default.
- W2317857526 cites W1627262848 @default.
- W2317857526 cites W1799635531 @default.
- W2317857526 cites W1965280193 @default.
- W2317857526 cites W1967465227 @default.
- W2317857526 cites W1969770714 @default.
- W2317857526 cites W1970156673 @default.
- W2317857526 cites W1981585431 @default.
- W2317857526 cites W1984357463 @default.
- W2317857526 cites W1986234964 @default.
- W2317857526 cites W1986619463 @default.
- W2317857526 cites W1987122345 @default.
- W2317857526 cites W1995875735 @default.
- W2317857526 cites W1998300401 @default.
- W2317857526 cites W1999909705 @default.
- W2317857526 cites W2008659594 @default.
- W2317857526 cites W2008900336 @default.
- W2317857526 cites W2009566340 @default.
- W2317857526 cites W2011282682 @default.
- W2317857526 cites W2020257254 @default.
- W2317857526 cites W2025729362 @default.
- W2317857526 cites W2026882865 @default.
- W2317857526 cites W2033325031 @default.
- W2317857526 cites W2034206030 @default.
- W2317857526 cites W2034229618 @default.
- W2317857526 cites W2034892963 @default.
- W2317857526 cites W2036369624 @default.
- W2317857526 cites W2037697367 @default.
- W2317857526 cites W2037940015 @default.
- W2317857526 cites W2038077039 @default.
- W2317857526 cites W2041877620 @default.
- W2317857526 cites W2046601975 @default.
- W2317857526 cites W2048523814 @default.
- W2317857526 cites W2048869777 @default.
- W2317857526 cites W2050249780 @default.
- W2317857526 cites W2056572892 @default.
- W2317857526 cites W2068011423 @default.
- W2317857526 cites W2068140368 @default.
- W2317857526 cites W2070310544 @default.
- W2317857526 cites W2073850887 @default.
- W2317857526 cites W2074231493 @default.
- W2317857526 cites W2076446466 @default.
- W2317857526 cites W2077861918 @default.
- W2317857526 cites W2078958918 @default.
- W2317857526 cites W2079450159 @default.
- W2317857526 cites W2084908610 @default.
- W2317857526 cites W2091851394 @default.
- W2317857526 cites W2096525273 @default.
- W2317857526 cites W2099388441 @default.
- W2317857526 cites W2101025813 @default.
- W2317857526 cites W2103735500 @default.
- W2317857526 cites W2106044656 @default.
- W2317857526 cites W2109745159 @default.
- W2317857526 cites W2114703458 @default.
- W2317857526 cites W2115772919 @default.
- W2317857526 cites W2117168988 @default.
- W2317857526 cites W2123246614 @default.
- W2317857526 cites W2126466006 @default.
- W2317857526 cites W2126602684 @default.
- W2317857526 cites W2127521865 @default.
- W2317857526 cites W2129314778 @default.
- W2317857526 cites W2129448726 @default.
- W2317857526 cites W2129873761 @default.
- W2317857526 cites W2129942096 @default.
- W2317857526 cites W2132348994 @default.
- W2317857526 cites W2134153749 @default.
- W2317857526 cites W2134193463 @default.
- W2317857526 cites W2135221645 @default.
- W2317857526 cites W2140158396 @default.
- W2317857526 cites W2140854179 @default.
- W2317857526 cites W2141652419 @default.
- W2317857526 cites W2142204199 @default.
- W2317857526 cites W2142418779 @default.
- W2317857526 cites W2142460132 @default.
- W2317857526 cites W2144710927 @default.
- W2317857526 cites W2147055980 @default.
- W2317857526 cites W2148640876 @default.