FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2317963773> ?p ?o ?g. }

• W2317963773 endingPage "A32" @default.
• W2317963773 startingPage "A32" @default.
• W2317963773 abstract "Colorectal cancer (CRC) is the second leading cause of cancer-related deaths. Overall, CRC is the third most diagnosed cancer in the United States with 150,000 new cases per year. Traditional CRC therapies are associated with severe toxicity, and therefore, natural products are appealing alternatives. Grape seed extract (GSE) is a complex polyphenolic mixture whose beneficial effects have been well documented across species and with various cancer models. Most (∼75%) of CRC develops sporadically, and diet, lifestyle, and/or environmental factors could further contribute to CRC progression. Chemoprevention utilizes an agent, natural or synthetic, to delay or stop cancer progression. Taken together the above discussion, here in we investigate chemoprevention potential of GSE against the Azoxymethane (AOM)-induced colon tumorigenesis. In our study, 6-week old A/J mice were divided into 5 groups: Group 1 received control diet, Group 2 received 0.5% GSE in diet, and the other 3 groups received i.p. injections of AOM (5mg/kg) weekly, for six weeks. Two weeks post AOM injections, one group continued on control diet and the other two groups were supplemented with 0.25% and 0.5% GSE (w/w) in diet. Animals were sacrificed at 32 and 42 weeks of age, or 20 and 30 weeks after last AOM injection. We found that GSE at 0.25% and 0.5% levels in diet did not exhibit toxic side effects with long-term treatment; and resulted in a 55% decrease in colon tumor multiplicity at 42 weeks at 0.5% dose. Furthermore, GSE treatment reduced overall tumor size; tumor burden ranging from 2-3mm was decreased by 85% in AOM-GSE-fed animals compared to control. Tissue analysis revealed GSE9 mechanisms of action: apoptotic death, and decreased proliferation and inflammation. Compared to controls, colon tissue from GSE-treated mice showed a 2-fold increase in TUNEL positive apoptotic cells, 30% decrease in PCNA positive proliferative cells, and ∼50% decrease in COX2 and iNOS levels in IHC analysis. Cytokine array of colon mucosal tissue revealed that compared to controls, GSE treatment resulted in approximately a 10-fold induction of GM-CSF, I-309, IL-1α, and BLC. In addition we observed a 10-30-fold decrease of IL-2, IL-1R, and IL-27 cytokine levels after GSE treatment. miRNA analysis of colon mucosal tissue revealed upregulation of miR-19a, miR-20a, and let7a with GSE treatment; a maximum 2500-fold induction was observed with miR-19a. GSE treatment also resulted in a 10-20-fold down regulation of miR-196a, miR-21, miR-148a, and miR-103. These results are consistence with our IHC observations of altered expression and localization of downstream players. GSE treatment resulted in decreased over-all expression of p21, β-catenin, c-myc, VEGF, and ERK 1/2. Altered localization of p21, β-catenin and c-myc was observed after GSE treatment resulting in increased cytosolic expression. In conclusion, our results indicated that GSE is already consumed by humans and is a non-toxic agent that exerts strong chemopreventive effect against sporadic colorectal cancer development, through modulation of apoptosis, proliferation, and inflammation pathways. Citation Format: Molly M. Derry, Komal Raina, Velmurugan Balaiya, Anil Jain, Sangeeta Shrotriya, Rajesh Agarwal, Chapla Agarwal. Chemopreventive potential of grape seed extract in azoxymethane-induced colon tumorigenesis in the A/J mouse model: Interlinking miRNA expression and inflammatory and cytokine signaling. [abstract]. In: Proceedings of the Eleventh Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2012 Oct 16-19; Anaheim, CA. Philadelphia (PA): AACR; Cancer Prev Res 2012;5(11 Suppl):Abstract nr A32." @default.
• W2317963773 created "2016-06-24" @default.
• W2317963773 creator A5007029060 @default.
• W2317963773 creator A5013163044 @default.
• W2317963773 creator A5018358676 @default.
• W2317963773 creator A5027734680 @default.
• W2317963773 creator A5048444823 @default.
• W2317963773 creator A5069980749 @default.
• W2317963773 creator A5071219911 @default.
• W2317963773 date "2012-11-01" @default.
• W2317963773 modified "2023-09-24" @default.
• W2317963773 title "Abstract A32: Chemopreventive potential of Grape Seed Extract in azoxymethane-induced colon tumorigenesis in the A/J mouse model: interlinking miRNA expression and inflammatory and cytokine signaling" @default.
• W2317963773 doi "https://doi.org/10.1158/1940-6207.prev-12-a32" @default.
• W2317963773 hasPublicationYear "2012" @default.
• W2317963773 type Work @default.
• W2317963773 sameAs 2317963773 @default.
• W2317963773 citedByCount "0" @default.
• W2317963773 crossrefType "journal-article" @default.
• W2317963773 hasAuthorship W2317963773A5007029060 @default.
• W2317963773 hasAuthorship W2317963773A5013163044 @default.
• W2317963773 hasAuthorship W2317963773A5018358676 @default.
• W2317963773 hasAuthorship W2317963773A5027734680 @default.
• W2317963773 hasAuthorship W2317963773A5048444823 @default.
• W2317963773 hasAuthorship W2317963773A5069980749 @default.
• W2317963773 hasAuthorship W2317963773A5071219911 @default.
• W2317963773 hasConcept C121608353 @default.
• W2317963773 hasConcept C126322002 @default.
• W2317963773 hasConcept C142724271 @default.
• W2317963773 hasConcept C143998085 @default.
• W2317963773 hasConcept C204787440 @default.
• W2317963773 hasConcept C2776971262 @default.
• W2317963773 hasConcept C2778452354 @default.
• W2317963773 hasConcept C502942594 @default.
• W2317963773 hasConcept C526805850 @default.
• W2317963773 hasConcept C555283112 @default.
• W2317963773 hasConcept C71924100 @default.
• W2317963773 hasConcept C90924648 @default.
• W2317963773 hasConcept C98274493 @default.
• W2317963773 hasConceptScore W2317963773C121608353 @default.
• W2317963773 hasConceptScore W2317963773C126322002 @default.
• W2317963773 hasConceptScore W2317963773C142724271 @default.
• W2317963773 hasConceptScore W2317963773C143998085 @default.
• W2317963773 hasConceptScore W2317963773C204787440 @default.
• W2317963773 hasConceptScore W2317963773C2776971262 @default.
• W2317963773 hasConceptScore W2317963773C2778452354 @default.
• W2317963773 hasConceptScore W2317963773C502942594 @default.
• W2317963773 hasConceptScore W2317963773C526805850 @default.
• W2317963773 hasConceptScore W2317963773C555283112 @default.
• W2317963773 hasConceptScore W2317963773C71924100 @default.
• W2317963773 hasConceptScore W2317963773C90924648 @default.
• W2317963773 hasConceptScore W2317963773C98274493 @default.
• W2317963773 hasIssue "11 Supplement" @default.
• W2317963773 hasLocation W23179637731 @default.
• W2317963773 hasOpenAccess W2317963773 @default.
• W2317963773 hasPrimaryLocation W23179637731 @default.
• W2317963773 hasRelatedWork W1520185676 @default.
• W2317963773 hasRelatedWork W1915079118 @default.
• W2317963773 hasRelatedWork W1994117241 @default.
• W2317963773 hasRelatedWork W2009233027 @default.
• W2317963773 hasRelatedWork W2018420824 @default.
• W2317963773 hasRelatedWork W2036574829 @default.
• W2317963773 hasRelatedWork W2036652348 @default.
• W2317963773 hasRelatedWork W2089506765 @default.
• W2317963773 hasRelatedWork W2117890784 @default.
• W2317963773 hasRelatedWork W2156145181 @default.
• W2317963773 hasRelatedWork W2314712454 @default.
• W2317963773 hasRelatedWork W2316158740 @default.
• W2317963773 hasRelatedWork W2327863002 @default.
• W2317963773 hasRelatedWork W2561172014 @default.
• W2317963773 hasRelatedWork W2740487699 @default.
• W2317963773 hasRelatedWork W2915225745 @default.
• W2317963773 hasRelatedWork W3033171989 @default.
• W2317963773 hasRelatedWork W3214374351 @default.
• W2317963773 hasRelatedWork W2560149717 @default.
• W2317963773 hasRelatedWork W3145467951 @default.
• W2317963773 hasVolume "5" @default.
• W2317963773 isParatext "false" @default.
• W2317963773 isRetracted "false" @default.
• W2317963773 magId "2317963773" @default.
• W2317963773 workType "article" @default.