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• W2318155488 endingPage "6431" @default.
• W2318155488 startingPage "6425" @default.
• W2318155488 abstract "Locust bean gum (LBG) was employed to screen mannanase-producing bacteria. The bacterium with highest mannanase ability was identified as Paenibacillus cookii. It revealed highest activity (6.67 U/mL) when cultivated in 0.1% LBG with 1.5% soytone and 0.5% tryptone after 4 days incubation at 27 °C. Its mannanase was purified to electrophoretical homogeneity after DEAE-Sepharose and Sephacryl S-100 separation. The purified mannanase, with an N-terminus of GLFGINAY, had pH and temperature optimum at 5.0 and 50 °C, respectively, and was stable at pH 5.0–7.0, ≤50 °C. It was strongly activated by β-mercaptoethanol, dithiothreitol, cysteine, and glutathione, but inhibited by Hg2+, Cu2+, Zn2+, Fe3+, PMSF, iodoacetic acid, and EDTA. According to substrate specificity study, the purified mannanase had high specificity to LBG and konjac." @default.
• W2318155488 created "2016-06-24" @default.
• W2318155488 creator A5011209322 @default.
• W2318155488 creator A5012300448 @default.
• W2318155488 creator A5022726784 @default.
• W2318155488 date "2012-06-19" @default.
• W2318155488 modified "2023-10-18" @default.
• W2318155488 title "Characterization of Mannanase from a Novel Mannanase-Producing Bacterium" @default.
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• W2318155488 doi "https://doi.org/10.1021/jf301944e" @default.
• W2318155488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22694324" @default.
• W2318155488 hasPublicationYear "2012" @default.
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