Matches in SemOpenAlex for { <https://semopenalex.org/work/W2318201257> ?p ?o ?g. }
- W2318201257 endingPage "15175" @default.
- W2318201257 startingPage "15170" @default.
- W2318201257 abstract "Prolyl hydroxylation and subsequent glycosylation of the E3(SCF) ubiquitin ligase subunit Skp1 affects its conformation and its interaction with F-box proteins and, ultimately, O2-sensing in the organism. Taking a reductionist approach to understand the molecular basis for these effects, a series of end-capped Thr-Pro dipeptides was synthesized, tracking the sequential post-translational modifications that occur in the protein. The conformation of the pyrrolidine ring in each compound was gauged via coupling constants ((3)JHα,Hβ) and the electronegativity of the Cγ-substituents by chemical shifts ((13)C). The equilibrium between the cis-trans conformations about the central prolyl peptide bond was investigated by integration of signals corresponding to the two species in the (1)H NMR spectra over a range of temperatures. These studies revealed an increasing preference for the trans-conformation in the order Pro < Hyp < [α-(1,4)GlcNAc]Hyp. Rates for the forward and reverse reactions, determined by magnetization transfer experiments, demonstrated a reduced rate for the trans-to-cis conversion and a significant increase in the cis-to-trans conversion upon hydroxylation of the proline residue in the dipeptide. NOE experiments suggest that the Thr side chain pushes the sugar away from the pyrrolidine ring. These effects, which depended on the presence of the N-terminal Thr residue, offer a mechanism to explain altered properties of the corresponding full-length proteins." @default.
- W2318201257 created "2016-06-24" @default.
- W2318201257 creator A5016593302 @default.
- W2318201257 creator A5025220460 @default.
- W2318201257 creator A5048635362 @default.
- W2318201257 creator A5090623717 @default.
- W2318201257 date "2014-10-16" @default.
- W2318201257 modified "2023-10-13" @default.
- W2318201257 title "Conformational Changes Associated with Post-Translational Modifications of Pro143 in Skp1 of Dictyostelium—A Dipeptide Model System" @default.
- W2318201257 cites W1519684943 @default.
- W2318201257 cites W1538719640 @default.
- W2318201257 cites W1603646817 @default.
- W2318201257 cites W1965884741 @default.
- W2318201257 cites W1967550539 @default.
- W2318201257 cites W1968072780 @default.
- W2318201257 cites W1968983628 @default.
- W2318201257 cites W1971139684 @default.
- W2318201257 cites W1971398376 @default.
- W2318201257 cites W1971763423 @default.
- W2318201257 cites W1973499604 @default.
- W2318201257 cites W1981595624 @default.
- W2318201257 cites W1981849277 @default.
- W2318201257 cites W1983741980 @default.
- W2318201257 cites W1994776365 @default.
- W2318201257 cites W1995992973 @default.
- W2318201257 cites W2000012049 @default.
- W2318201257 cites W2000430596 @default.
- W2318201257 cites W2002885008 @default.
- W2318201257 cites W2004226875 @default.
- W2318201257 cites W2009312115 @default.
- W2318201257 cites W2009458361 @default.
- W2318201257 cites W2011737846 @default.
- W2318201257 cites W2012744739 @default.
- W2318201257 cites W2015771185 @default.
- W2318201257 cites W2020821243 @default.
- W2318201257 cites W2021760309 @default.
- W2318201257 cites W2021798993 @default.
- W2318201257 cites W2030219315 @default.
- W2318201257 cites W2033416116 @default.
- W2318201257 cites W2037508684 @default.
- W2318201257 cites W2037963845 @default.
- W2318201257 cites W2039519959 @default.
- W2318201257 cites W2040119338 @default.
- W2318201257 cites W2050023137 @default.
- W2318201257 cites W2050922724 @default.
- W2318201257 cites W2051494500 @default.
- W2318201257 cites W2052737087 @default.
- W2318201257 cites W2054781768 @default.
- W2318201257 cites W2057980421 @default.
- W2318201257 cites W2062371417 @default.
- W2318201257 cites W2063070658 @default.
- W2318201257 cites W2066721894 @default.
- W2318201257 cites W2067736294 @default.
- W2318201257 cites W2071461485 @default.
- W2318201257 cites W2072522851 @default.
- W2318201257 cites W2073640163 @default.
- W2318201257 cites W2077448832 @default.
- W2318201257 cites W2078931933 @default.
- W2318201257 cites W2086550655 @default.
- W2318201257 cites W2100485404 @default.
- W2318201257 cites W2127803479 @default.
- W2318201257 cites W2130227523 @default.
- W2318201257 cites W2143857507 @default.
- W2318201257 cites W2144097678 @default.
- W2318201257 cites W2144114582 @default.
- W2318201257 cites W2157495692 @default.
- W2318201257 cites W2160648204 @default.
- W2318201257 cites W2168889822 @default.
- W2318201257 cites W2170823043 @default.
- W2318201257 cites W2321910107 @default.
- W2318201257 cites W4242009576 @default.
- W2318201257 cites W4247801929 @default.
- W2318201257 doi "https://doi.org/10.1021/ja5033277" @default.
- W2318201257 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4227711" @default.
- W2318201257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25250945" @default.
- W2318201257 hasPublicationYear "2014" @default.
- W2318201257 type Work @default.
- W2318201257 sameAs 2318201257 @default.
- W2318201257 citedByCount "6" @default.
- W2318201257 countsByYear W23182012572015 @default.
- W2318201257 countsByYear W23182012572017 @default.
- W2318201257 countsByYear W23182012572019 @default.
- W2318201257 crossrefType "journal-article" @default.
- W2318201257 hasAuthorship W2318201257A5016593302 @default.
- W2318201257 hasAuthorship W2318201257A5025220460 @default.
- W2318201257 hasAuthorship W2318201257A5048635362 @default.
- W2318201257 hasAuthorship W2318201257A5090623717 @default.
- W2318201257 hasBestOaLocation W23182012571 @default.
- W2318201257 hasConcept C104317684 @default.
- W2318201257 hasConcept C134459356 @default.
- W2318201257 hasConcept C181199279 @default.
- W2318201257 hasConcept C185592680 @default.
- W2318201257 hasConcept C25602115 @default.
- W2318201257 hasConcept C2778851528 @default.
- W2318201257 hasConcept C2779138802 @default.
- W2318201257 hasConcept C2779281246 @default.
- W2318201257 hasConcept C2781109383 @default.
- W2318201257 hasConcept C31684184 @default.