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- W2318223889 abstract "Standard approaches to the treatment of cancer, including surgery, chemotherapy, and radiation, when used alone or in combination, have had limited efficacy in curing the disease. These treatments frequently produce debilitating side effects, such as nausea, vomiting, loss of hair, and suppression of immunity. Due to these side effects, interest in other cancer treatments, including herbal extracts, has increased. Traditional herbal extracts are considered to be safe and economically feasible as alternative medicines to treat cancer. An alcoholic extract of the roots of Withania somnifera (WS), a traditional herbal remedy, has immunostimulatory, radiosensitizing, and anticancer effects. Knowledge of the medicinal properties of this plant motivated us to investigate its efficacy as an antitumor agent against breast cancer MDA-MB-231 human breast cancer cells were used as a model. Tests included an MTT assay for cell viability, an assay for testing of migration of cancer cells, PCR analysis for cytokines, evaluation of gene expression, an immunohistopathological assay for expression of cytokines in isolated tissues, and xenografts in nude mice to evaluate inhibition of tumor growth. In cell cultures, WS showed antiproliferative and anti-invasive effects against MDA-MB-231 breast cancer cells; it also inhibited the metastasis of xenografted MDA-MB-231 cells to the lungs of treated mice. These findings were substantiated in vitro by use of a microarray technique. WS caused down-regulation of cytokines CCL2, CXCL1, CXCL2, CXCL3, IL-1b, and TGF-β3. CCL2 is the cytokine considered to be most responsible for the metastasis of breast cancer cells. The inhibition of CCL2 by WS was confirmed in sections of xenografted treated tumors and in cell culture by use of immunohistochemical and flow cytometry techniques, respectively. WS caused 50% inhibition of CD44-bearing breast cancer stem cells, the predominant stem cells of the MDA-MB-231 breast cancer cell line. This finding will be further pursued by sorting CD44high/CD24low stem cells by use of flow cytometry. In summary, WS caused inhibition of proliferation and invasion of breast cancer cells in culture and growth of xenografted cancer cells in mice. These effects may be associated with inhibition of cytokines such as CCL2 and CD 44high/CD 24low s. Citation Information: Cancer Res 2013;73(24 Suppl): Abstract nr P6-09-08." @default.
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- W2318223889 date "2013-12-15" @default.
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- W2318223889 title "Abstract P6-09-08: Withania somnifera extract inhibits invasion and metastasis of MDA-MB-231 cells by inhibiting CCL2 and CD44 high/CD24low stem cells" @default.
- W2318223889 doi "https://doi.org/10.1158/0008-5472.sabcs13-p6-09-08" @default.
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