Matches in SemOpenAlex for { <https://semopenalex.org/work/W2318242502> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W2318242502 abstract "Four N-acetyl-β-D-glucosaminides, 4-amino-2, 6-dibromophenyl (1a), 4-amino-2, 6-dichlorophenyl (1b), 4-amino-2-chlorophenyl (1c) and 4-aminophenyl N-acetyl-β-D-glucosaminides (1d) were synthesized. Substrates 1a-c were hydrolyzed by N-acetyl-β-D-glucosaminidase and the released aglycones reacted with N-ethyl-N-(3-methylphenyl)-N'-succinylethylenediamine to produce indoaniline dyes in the presence of bilirubin oxidase under weakly acidic rate-assay conditions (pH 5.0). The Km values for 1a-c were 1.97, 1.65 and 1.39 mM, respectively. Among these compounds, 1b is considered to be the substrate with most potential for the rate-assay of N-acetyl-β-D-glucosaminidase, since it showed the largest Vmax value and the strongest color generation from colorless to green (λmax 302→718 nm)following enzyme hydrolysis and the coupling reaction. Furthermore, 1b was moderately soluble and stable in aqueous solution and exhibited about a 5.9-fold higher sensitivity to the enzyme than 2-chloro-4-nitrophenyl N-acetyl-β-D-glucosaminide." @default.
- W2318242502 created "2016-06-24" @default.
- W2318242502 creator A5039022969 @default.
- W2318242502 creator A5072729039 @default.
- W2318242502 creator A5079298506 @default.
- W2318242502 date "1995-01-01" @default.
- W2318242502 modified "2023-09-23" @default.
- W2318242502 title "Synthesis of 4-Aminophenyl N-Acetyl-.BETA.-D-glucosaminide Derivatives and Their Application to the Rate-Assay of N-Acetyl-.BETA.-D-glucosaminidase." @default.
- W2318242502 cites W1566830427 @default.
- W2318242502 doi "https://doi.org/10.1248/cpb.43.266" @default.
- W2318242502 hasPublicationYear "1995" @default.
- W2318242502 type Work @default.
- W2318242502 sameAs 2318242502 @default.
- W2318242502 citedByCount "6" @default.
- W2318242502 countsByYear W23182425022015 @default.
- W2318242502 crossrefType "journal-article" @default.
- W2318242502 hasAuthorship W2318242502A5039022969 @default.
- W2318242502 hasAuthorship W2318242502A5072729039 @default.
- W2318242502 hasAuthorship W2318242502A5079298506 @default.
- W2318242502 hasBestOaLocation W23182425021 @default.
- W2318242502 hasConcept C111368507 @default.
- W2318242502 hasConcept C127313418 @default.
- W2318242502 hasConcept C13965031 @default.
- W2318242502 hasConcept C155647269 @default.
- W2318242502 hasConcept C178790620 @default.
- W2318242502 hasConcept C181199279 @default.
- W2318242502 hasConcept C184651966 @default.
- W2318242502 hasConcept C185592680 @default.
- W2318242502 hasConcept C2777289219 @default.
- W2318242502 hasConcept C2777915509 @default.
- W2318242502 hasConcept C38485361 @default.
- W2318242502 hasConcept C43617362 @default.
- W2318242502 hasConcept C71240020 @default.
- W2318242502 hasConcept C94412978 @default.
- W2318242502 hasConceptScore W2318242502C111368507 @default.
- W2318242502 hasConceptScore W2318242502C127313418 @default.
- W2318242502 hasConceptScore W2318242502C13965031 @default.
- W2318242502 hasConceptScore W2318242502C155647269 @default.
- W2318242502 hasConceptScore W2318242502C178790620 @default.
- W2318242502 hasConceptScore W2318242502C181199279 @default.
- W2318242502 hasConceptScore W2318242502C184651966 @default.
- W2318242502 hasConceptScore W2318242502C185592680 @default.
- W2318242502 hasConceptScore W2318242502C2777289219 @default.
- W2318242502 hasConceptScore W2318242502C2777915509 @default.
- W2318242502 hasConceptScore W2318242502C38485361 @default.
- W2318242502 hasConceptScore W2318242502C43617362 @default.
- W2318242502 hasConceptScore W2318242502C71240020 @default.
- W2318242502 hasConceptScore W2318242502C94412978 @default.
- W2318242502 hasLocation W23182425021 @default.
- W2318242502 hasOpenAccess W2318242502 @default.
- W2318242502 hasPrimaryLocation W23182425021 @default.
- W2318242502 hasRelatedWork W1979013829 @default.
- W2318242502 hasRelatedWork W1979963222 @default.
- W2318242502 hasRelatedWork W1989254830 @default.
- W2318242502 hasRelatedWork W1991719792 @default.
- W2318242502 hasRelatedWork W1991831292 @default.
- W2318242502 hasRelatedWork W2003475957 @default.
- W2318242502 hasRelatedWork W2013377060 @default.
- W2318242502 hasRelatedWork W2029878807 @default.
- W2318242502 hasRelatedWork W2043808782 @default.
- W2318242502 hasRelatedWork W2047030426 @default.
- W2318242502 hasRelatedWork W2093007329 @default.
- W2318242502 hasRelatedWork W2096023560 @default.
- W2318242502 hasRelatedWork W2148135543 @default.
- W2318242502 hasRelatedWork W2335103015 @default.
- W2318242502 hasRelatedWork W2335570585 @default.
- W2318242502 hasRelatedWork W2401599321 @default.
- W2318242502 hasRelatedWork W319706349 @default.
- W2318242502 hasRelatedWork W194695176 @default.
- W2318242502 hasRelatedWork W2087832958 @default.
- W2318242502 hasRelatedWork W2478571196 @default.
- W2318242502 isParatext "false" @default.
- W2318242502 isRetracted "false" @default.
- W2318242502 magId "2318242502" @default.
- W2318242502 workType "article" @default.