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• W2318367165 abstract "Isolation of herpes simplex virus (HSV) in cell culture, in parallel with neutralizing (NT) antibody assay in paired sera, was carried out in specimens collected from oral lesions of 73 outpatients with clinical signs of HSV infection at the Department of Oral Surgery, Yokohama City University Hospital.Of 47 samples collected within 4 days of outbreak of symptoms, 34 were positive for HSV, but isolation rate decreased gradually depending on time of sample collection and reached 20% in samples taken after 12 days. In general the virus was isolated more easily from primary cases than from cases of recurrence.Clinically, ulcer was predominant (53%) in primary cases, and scattered vesicles were more commonly observed (49%) in recurrence. This difference seems to reflect the histological characteristics of the regions, i.e., mucous membrane and skin, where these infections tend to appear. NT-antibody in primary cases was first detectable 3 to 9 days after onset, and reached a maximum and plateau level in 2 to 3 weeks. On the other hand, antibody titer was not different in either acute or convalescent phase in most of the recurrent cases.From our experience we concluded that the collection of specimens from lesions should be done as soon as possible after onset of clinical manifestations to get successful results in isolation, and sera should be collected within 7 days of onset of illness for the acute phase and after 14 days for the convalescent phase to identify primary infection from recurrence by means of NT antibody titers." @default.
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• W2318367165 date "1990-01-01" @default.
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• W2318367165 title "Isolation of herpes simplex virus from oral lesions of patients and characterization of antibody response during course of infection." @default.
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• W2318367165 doi "https://doi.org/10.5794/jjoms.36.858" @default.
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