Matches in SemOpenAlex for { <https://semopenalex.org/work/W2318379633> ?p ?o ?g. }
- W2318379633 endingPage "295" @default.
- W2318379633 startingPage "286" @default.
- W2318379633 abstract "Hydrogen exchange mass spectrometry (HXMS) coupled to proteolytic digestion has been used to probe the conformation of bovine β-lactoglobulin (BLG), bovine α-lactalbumin (BLA), and human serum albumin (HSA) in solution and while adsorbed to the hydrophobic interaction chromatography media Phenyl Sepharose 6FF. All three proteins show evidence of EX1 exchange kinetics, indicating a loss of stability on the surface. HX protection patterns for all three proteins also indicate that the unfolded form is only partially solvent exposed. The hydrogen-deuterium exchange patterns of BLG and BLA on the surface suggest a structure that resembles each protein's respective solution phase molten globule state. The low stability of Domain II of HSA observed on Phenyl Sepharose 6FF also suggests a link to solution stability because Domain II is frequently cited as the least stable domain in solution unfolding pathways. COREX, an algorithm used to compute protein folding stabilities, correctly predicts solution hydrogen-deuterium exchange patterns for BLG and offers insight into its adsorbed phase stabilities but is unreliable for BLA predictions. The results of this work demonstrate a link between solution-phase local stability patterns and the nature of partially unfolded states that proteins can adopt on HIC surfaces." @default.
- W2318379633 created "2016-06-24" @default.
- W2318379633 creator A5026977999 @default.
- W2318379633 creator A5050823024 @default.
- W2318379633 creator A5062530827 @default.
- W2318379633 creator A5065822245 @default.
- W2318379633 date "2010-11-30" @default.
- W2318379633 modified "2023-10-18" @default.
- W2318379633 title "Protein Stability and Structure in HIC: Hydrogen Exchange Experiments and COREX Calculations" @default.
- W2318379633 cites W114432466 @default.
- W2318379633 cites W1499408335 @default.
- W2318379633 cites W1552697504 @default.
- W2318379633 cites W1574852629 @default.
- W2318379633 cites W1583149693 @default.
- W2318379633 cites W1589367648 @default.
- W2318379633 cites W1951398569 @default.
- W2318379633 cites W1963729774 @default.
- W2318379633 cites W1967614917 @default.
- W2318379633 cites W1990047565 @default.
- W2318379633 cites W1990126142 @default.
- W2318379633 cites W1997702098 @default.
- W2318379633 cites W2000029728 @default.
- W2318379633 cites W2013965701 @default.
- W2318379633 cites W2019419950 @default.
- W2318379633 cites W2021005100 @default.
- W2318379633 cites W2024446274 @default.
- W2318379633 cites W2030459292 @default.
- W2318379633 cites W2034741330 @default.
- W2318379633 cites W2038962652 @default.
- W2318379633 cites W2044294967 @default.
- W2318379633 cites W2045644540 @default.
- W2318379633 cites W2053083611 @default.
- W2318379633 cites W2059843972 @default.
- W2318379633 cites W2071075252 @default.
- W2318379633 cites W2075454119 @default.
- W2318379633 cites W2077382505 @default.
- W2318379633 cites W2078177368 @default.
- W2318379633 cites W2078802956 @default.
- W2318379633 cites W2080736062 @default.
- W2318379633 cites W2081085903 @default.
- W2318379633 cites W2081413441 @default.
- W2318379633 cites W2085078763 @default.
- W2318379633 cites W2085832177 @default.
- W2318379633 cites W2091956372 @default.
- W2318379633 cites W2114320763 @default.
- W2318379633 cites W2128954946 @default.
- W2318379633 cites W2131911094 @default.
- W2318379633 cites W2131918208 @default.
- W2318379633 cites W2141929258 @default.
- W2318379633 cites W2150025608 @default.
- W2318379633 cites W2158440279 @default.
- W2318379633 cites W2165746966 @default.
- W2318379633 cites W2171109878 @default.
- W2318379633 cites W2171443541 @default.
- W2318379633 cites W2319633358 @default.
- W2318379633 doi "https://doi.org/10.1021/la103793r" @default.
- W2318379633 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21117672" @default.
- W2318379633 hasPublicationYear "2010" @default.
- W2318379633 type Work @default.
- W2318379633 sameAs 2318379633 @default.
- W2318379633 citedByCount "13" @default.
- W2318379633 countsByYear W23183796332012 @default.
- W2318379633 countsByYear W23183796332014 @default.
- W2318379633 countsByYear W23183796332015 @default.
- W2318379633 countsByYear W23183796332016 @default.
- W2318379633 countsByYear W23183796332017 @default.
- W2318379633 countsByYear W23183796332018 @default.
- W2318379633 countsByYear W23183796332019 @default.
- W2318379633 crossrefType "journal-article" @default.
- W2318379633 hasAuthorship W2318379633A5026977999 @default.
- W2318379633 hasAuthorship W2318379633A5050823024 @default.
- W2318379633 hasAuthorship W2318379633A5062530827 @default.
- W2318379633 hasAuthorship W2318379633A5065822245 @default.
- W2318379633 hasConcept C118493188 @default.
- W2318379633 hasConcept C119599485 @default.
- W2318379633 hasConcept C121332964 @default.
- W2318379633 hasConcept C127413603 @default.
- W2318379633 hasConcept C147789679 @default.
- W2318379633 hasConcept C150394285 @default.
- W2318379633 hasConcept C162356407 @default.
- W2318379633 hasConcept C167569220 @default.
- W2318379633 hasConcept C178790620 @default.
- W2318379633 hasConcept C185592680 @default.
- W2318379633 hasConcept C204328495 @default.
- W2318379633 hasConcept C2776545253 @default.
- W2318379633 hasConcept C2778597219 @default.
- W2318379633 hasConcept C43617362 @default.
- W2318379633 hasConcept C44280652 @default.
- W2318379633 hasConcept C55493867 @default.
- W2318379633 hasConcept C58364064 @default.
- W2318379633 hasConcept C62520636 @default.
- W2318379633 hasConcept C68863886 @default.
- W2318379633 hasConcept C8010536 @default.
- W2318379633 hasConcept C86807702 @default.
- W2318379633 hasConcept C90260826 @default.
- W2318379633 hasConceptScore W2318379633C118493188 @default.
- W2318379633 hasConceptScore W2318379633C119599485 @default.
- W2318379633 hasConceptScore W2318379633C121332964 @default.