Matches in SemOpenAlex for { <https://semopenalex.org/work/W2318392488> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2318392488 endingPage "620" @default.
- W2318392488 startingPage "620" @default.
- W2318392488 abstract "Specific-pathogen-free sentinel birds were used as an initial biological system to isolate infectious bursal disease virus (IBDV) field isolates from commercial broiler farms exhibiting recurrent respiratory problems and poor performance. Reverse transcription (RT)-polymerase chain reaction (PCR) was used to amplify a 248-bp product encompassing the hypervariable region of the IBDV VP2 gene. Restriction fragment length polymorphism (RFLP) analysis of the RT-PCR products was performed with the restriction endonucleases DraI, SadI, TaqI, StyI, BstNI, and SspI. Two isolates (619 and 850) exhibited a RFLP pattern characteristic of Delaware variant E IBDV. Restriction enzyme digestion for four isolates (625, 849, 853, and 11,153) revealed unmatched RFLP patterns when compared with reference IBDV strains. Nucleotide and deduced amino acid sequence analyses of the VP2 hypervariable region for these six isolates revealed identity (96.3% up to 98%) with Delaware E variant IBDV strain. However, serine at position 254, which is characteristic of Delaware variant strains, was substituted by asparagine in these six isolates. The seventh IBDV isolate (9109) also exhibited a unique RFLP pattern, which included the SspI restriction site, which is characteristic of very virulent (vv) IBDV strains. Nucleotide and amino acid sequence analyses of the hypervariable region for this isolate revealed identity (90%) with the standard challenge strain. However, the leucine residue at position 294 was substituted by isoleucine. This substitution corresponds to one of the amino acids that are conserved in the vvIBDV strains. Antigenic index studies of the predicted amino acid sequence of the hypervariable region of VP2 from isolates 619, 625, 849, 850, 853, and 11,153 exhibited a profile almost identical to variant E, whereas the isolate 9109 exhibited a profile characteristic of standard IBDV strains." @default.
- W2318392488 created "2016-06-24" @default.
- W2318392488 creator A5040682790 @default.
- W2318392488 creator A5043956255 @default.
- W2318392488 creator A5051296706 @default.
- W2318392488 creator A5071192415 @default.
- W2318392488 date "2001-07-01" @default.
- W2318392488 modified "2023-10-02" @default.
- W2318392488 title "Molecular Characterization of Seven Field Isolates of Infectious Bursal Disease Virus Obtained from Commercial Broiler Chickens" @default.
- W2318392488 cites W124790931 @default.
- W2318392488 cites W1604809493 @default.
- W2318392488 cites W1974417513 @default.
- W2318392488 cites W1977696068 @default.
- W2318392488 cites W1980605992 @default.
- W2318392488 cites W1983805470 @default.
- W2318392488 cites W2006885958 @default.
- W2318392488 cites W2017504860 @default.
- W2318392488 cites W2026042432 @default.
- W2318392488 cites W2036818420 @default.
- W2318392488 cites W2045710497 @default.
- W2318392488 cites W2045954345 @default.
- W2318392488 cites W2075779452 @default.
- W2318392488 cites W2078919721 @default.
- W2318392488 cites W2099619575 @default.
- W2318392488 cites W2134812217 @default.
- W2318392488 cites W2138270253 @default.
- W2318392488 cites W2140998261 @default.
- W2318392488 cites W2313160691 @default.
- W2318392488 cites W2316257070 @default.
- W2318392488 cites W2318292049 @default.
- W2318392488 cites W2319963805 @default.
- W2318392488 cites W2322015552 @default.
- W2318392488 cites W2323682653 @default.
- W2318392488 cites W2328127097 @default.
- W2318392488 cites W2330785784 @default.
- W2318392488 cites W2335731686 @default.
- W2318392488 cites W2586548128 @default.
- W2318392488 cites W2950562600 @default.
- W2318392488 doi "https://doi.org/10.2307/1592903" @default.
- W2318392488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11569735" @default.
- W2318392488 hasPublicationYear "2001" @default.
- W2318392488 type Work @default.
- W2318392488 sameAs 2318392488 @default.
- W2318392488 citedByCount "32" @default.
- W2318392488 countsByYear W23183924882012 @default.
- W2318392488 countsByYear W23183924882013 @default.
- W2318392488 countsByYear W23183924882014 @default.
- W2318392488 countsByYear W23183924882015 @default.
- W2318392488 countsByYear W23183924882023 @default.
- W2318392488 crossrefType "journal-article" @default.
- W2318392488 hasAuthorship W2318392488A5040682790 @default.
- W2318392488 hasAuthorship W2318392488A5043956255 @default.
- W2318392488 hasAuthorship W2318392488A5051296706 @default.
- W2318392488 hasAuthorship W2318392488A5071192415 @default.
- W2318392488 hasConcept C104317684 @default.
- W2318392488 hasConcept C128319531 @default.
- W2318392488 hasConcept C159047783 @default.
- W2318392488 hasConcept C168007829 @default.
- W2318392488 hasConcept C2522874641 @default.
- W2318392488 hasConcept C2777810641 @default.
- W2318392488 hasConcept C49105822 @default.
- W2318392488 hasConcept C54355233 @default.
- W2318392488 hasConcept C60987743 @default.
- W2318392488 hasConcept C81566733 @default.
- W2318392488 hasConcept C84148353 @default.
- W2318392488 hasConcept C86803240 @default.
- W2318392488 hasConceptScore W2318392488C104317684 @default.
- W2318392488 hasConceptScore W2318392488C128319531 @default.
- W2318392488 hasConceptScore W2318392488C159047783 @default.
- W2318392488 hasConceptScore W2318392488C168007829 @default.
- W2318392488 hasConceptScore W2318392488C2522874641 @default.
- W2318392488 hasConceptScore W2318392488C2777810641 @default.
- W2318392488 hasConceptScore W2318392488C49105822 @default.
- W2318392488 hasConceptScore W2318392488C54355233 @default.
- W2318392488 hasConceptScore W2318392488C60987743 @default.
- W2318392488 hasConceptScore W2318392488C81566733 @default.
- W2318392488 hasConceptScore W2318392488C84148353 @default.
- W2318392488 hasConceptScore W2318392488C86803240 @default.
- W2318392488 hasIssue "3" @default.
- W2318392488 hasLocation W23183924881 @default.
- W2318392488 hasLocation W23183924882 @default.
- W2318392488 hasOpenAccess W2318392488 @default.
- W2318392488 hasPrimaryLocation W23183924881 @default.
- W2318392488 hasRelatedWork W1965958885 @default.
- W2318392488 hasRelatedWork W2013840905 @default.
- W2318392488 hasRelatedWork W2067484826 @default.
- W2318392488 hasRelatedWork W2081466306 @default.
- W2318392488 hasRelatedWork W2176863991 @default.
- W2318392488 hasRelatedWork W2177205479 @default.
- W2318392488 hasRelatedWork W2179540004 @default.
- W2318392488 hasRelatedWork W2318392488 @default.
- W2318392488 hasRelatedWork W2335700489 @default.
- W2318392488 hasRelatedWork W4251232847 @default.
- W2318392488 hasVolume "45" @default.
- W2318392488 isParatext "false" @default.
- W2318392488 isRetracted "false" @default.
- W2318392488 magId "2318392488" @default.
- W2318392488 workType "article" @default.