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- W23184558 abstract "RNA expression profiles produced by next-generation sequencing (NGS) technology (RNA-seq) allow comprehensive investigation of transcribed sequences within a cell or tissue. RNA-seq is rapidly becoming more cost-effective for transcriptome profiling. However, its usage will expand dramatically if one starts with low amount of RNA and obtains transcript directionality during the analysis. Here, we describe a detailed protocol for the creation of a directional RNA-seq library from 100 ng of starting total RNA." @default.
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- W23184558 date "2012-01-01" @default.
- W23184558 modified "2023-10-16" @default.
- W23184558 title "Retinal Transcriptome Profiling by Directional Next-Generation Sequencing Using 100 ng of Total RNA" @default.
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- W23184558 doi "https://doi.org/10.1007/978-1-61779-848-1_23" @default.
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