Matches in SemOpenAlex for { <https://semopenalex.org/work/W2318541013> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2318541013 endingPage "180" @default.
- W2318541013 startingPage "174" @default.
- W2318541013 abstract "Food consumption is an important behavior that is regulated by an intricate array of neuropeptides (NPs). Although many feeding-related NPs have been identified in mammals, precise mechanisms are unclear and difficult to study in mammals, as current methods are not highly multiplexed and require extensive a priori knowledge about analytes. New advances in data-independent acquisition (DIA) MS/MS and the open-source quantification software Skyline have opened up the possibility to identify hundreds of compounds and quantify them from a single DIA MS/MS run. An untargeted DIA MS(E) quantification method using Skyline software for multiplexed, discovery-driven quantification was developed and found to produce linear calibration curves for peptides at physiologically relevant concentrations using a protein digest as internal standard. By using this method, preliminary relative quantification of the crab Cancer borealis neuropeptidome (<2 kDa, 137 peptides from 18 families) was possible in microdialysates from 8 replicate feeding experiments. Of these NPs, 55 were detected with an average mass error below 10 ppm. The time-resolved profiles of relative concentration changes for 6 are shown, and there is great potential for the use of this method in future experiments to aid in correlation of NP changes with behavior. This work presents an unbiased approach to winnowing candidate NPs related to a behavior of interest in a functionally relevant manner, and demonstrates the success of such a UPLC-MS(E) quantification method using the open source software Skyline." @default.
- W2318541013 created "2016-06-24" @default.
- W2318541013 creator A5049426351 @default.
- W2318541013 creator A5052707224 @default.
- W2318541013 creator A5071820359 @default.
- W2318541013 date "2015-01-08" @default.
- W2318541013 modified "2023-10-18" @default.
- W2318541013 title "Data-Independent MS/MS Quantification of Neuropeptides for Determination of Putative Feeding-Related Neurohormones in Microdialysate" @default.
- W2318541013 cites W1599273350 @default.
- W2318541013 cites W1974337531 @default.
- W2318541013 cites W1981536086 @default.
- W2318541013 cites W1995403127 @default.
- W2318541013 cites W2002124212 @default.
- W2318541013 cites W2022964472 @default.
- W2318541013 cites W2035751607 @default.
- W2318541013 cites W2038446484 @default.
- W2318541013 cites W2039058260 @default.
- W2318541013 cites W2043896568 @default.
- W2318541013 cites W2048744060 @default.
- W2318541013 cites W2053158702 @default.
- W2318541013 cites W2054531311 @default.
- W2318541013 cites W2055394595 @default.
- W2318541013 cites W2060810496 @default.
- W2318541013 cites W2062143232 @default.
- W2318541013 cites W2065950058 @default.
- W2318541013 cites W2079843936 @default.
- W2318541013 cites W2083847379 @default.
- W2318541013 cites W2086983886 @default.
- W2318541013 cites W2090469571 @default.
- W2318541013 cites W2115673413 @default.
- W2318541013 cites W2127704287 @default.
- W2318541013 cites W2141091417 @default.
- W2318541013 cites W2159254892 @default.
- W2318541013 cites W2159375452 @default.
- W2318541013 cites W2168434032 @default.
- W2318541013 cites W2416569999 @default.
- W2318541013 doi "https://doi.org/10.1021/cn500253u" @default.
- W2318541013 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4304520" @default.
- W2318541013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25552291" @default.
- W2318541013 hasPublicationYear "2015" @default.
- W2318541013 type Work @default.
- W2318541013 sameAs 2318541013 @default.
- W2318541013 citedByCount "12" @default.
- W2318541013 countsByYear W23185410132015 @default.
- W2318541013 countsByYear W23185410132016 @default.
- W2318541013 countsByYear W23185410132019 @default.
- W2318541013 countsByYear W23185410132021 @default.
- W2318541013 countsByYear W23185410132023 @default.
- W2318541013 crossrefType "journal-article" @default.
- W2318541013 hasAuthorship W2318541013A5049426351 @default.
- W2318541013 hasAuthorship W2318541013A5052707224 @default.
- W2318541013 hasAuthorship W2318541013A5071820359 @default.
- W2318541013 hasBestOaLocation W23185410131 @default.
- W2318541013 hasConcept C10390740 @default.
- W2318541013 hasConcept C105795698 @default.
- W2318541013 hasConcept C185592680 @default.
- W2318541013 hasConcept C2781162219 @default.
- W2318541013 hasConcept C33923547 @default.
- W2318541013 hasConcept C41008148 @default.
- W2318541013 hasConcept C43617362 @default.
- W2318541013 hasConcept C70721500 @default.
- W2318541013 hasConcept C86803240 @default.
- W2318541013 hasConceptScore W2318541013C10390740 @default.
- W2318541013 hasConceptScore W2318541013C105795698 @default.
- W2318541013 hasConceptScore W2318541013C185592680 @default.
- W2318541013 hasConceptScore W2318541013C2781162219 @default.
- W2318541013 hasConceptScore W2318541013C33923547 @default.
- W2318541013 hasConceptScore W2318541013C41008148 @default.
- W2318541013 hasConceptScore W2318541013C43617362 @default.
- W2318541013 hasConceptScore W2318541013C70721500 @default.
- W2318541013 hasConceptScore W2318541013C86803240 @default.
- W2318541013 hasFunder F4320337357 @default.
- W2318541013 hasIssue "1" @default.
- W2318541013 hasLocation W23185410131 @default.
- W2318541013 hasLocation W23185410132 @default.
- W2318541013 hasLocation W23185410133 @default.
- W2318541013 hasLocation W23185410134 @default.
- W2318541013 hasOpenAccess W2318541013 @default.
- W2318541013 hasPrimaryLocation W23185410131 @default.
- W2318541013 hasRelatedWork W2065444835 @default.
- W2318541013 hasRelatedWork W2082860237 @default.
- W2318541013 hasRelatedWork W22115721 @default.
- W2318541013 hasRelatedWork W2211931904 @default.
- W2318541013 hasRelatedWork W2320312871 @default.
- W2318541013 hasRelatedWork W2321234655 @default.
- W2318541013 hasRelatedWork W2470062578 @default.
- W2318541013 hasRelatedWork W2952773340 @default.
- W2318541013 hasRelatedWork W3171007296 @default.
- W2318541013 hasRelatedWork W4254851101 @default.
- W2318541013 hasVolume "6" @default.
- W2318541013 isParatext "false" @default.
- W2318541013 isRetracted "false" @default.
- W2318541013 magId "2318541013" @default.
- W2318541013 workType "article" @default.