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• W2318703352 abstract "Heavy metals are known to be inhibitory and toxic to the activated-sludge microbial community in biological wastewater treatment plants. Toxicity screening of aqueous mixtures of these heavy metal ions in plant influent could use both chemical and biological methods. As a biological method, luminescent bacterial bioreporters offer the advantages of a simple test procedure and rapid response. Current biologically based methods for screening aqueous streams for toxicity are labor-intensive, inaccurate, or difficult to use in continuous monitoring applications. In the present study, a system was developed that is simple and easily automated. This system is based on the bacterium Shk1, a genetically engineered bioluminescent Pseudomonad whose parent strain was originally isolated from activated sludge. Compared with other bioluminescence-based systems (specifically, the Microtox assay), the system of the present study more accurately reflects the effects of the toxicity of common metal ions on activated-sludge respirometry without being overly sensitive to typical constituents of wastewater. The use of Shk1 as a bioluminescent reporter for heavy metal toxicity testing for the application of wastewater treatment influent toxicity screening is presented in this study." @default.
• W2318703352 created "2016-06-24" @default.
• W2318703352 creator A5040896028 @default.
• W2318703352 creator A5064205969 @default.
• W2318703352 date "2003-01-01" @default.
• W2318703352 modified "2023-10-14" @default.
• W2318703352 title "The Use of a Genetically Engineered <i>Pseudomonas</i> Species (Shk1) as a Bioluminescent Reporter for Heavy Metal Toxicity Screening in Wastewater Treatment Plant Influent" @default.
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• W2318703352 doi "https://doi.org/10.2175/106143003x140791" @default.
• W2318703352 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12683460" @default.
• W2318703352 hasPublicationYear "2003" @default.
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