FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2318756323> ?p ?o ?g. }

• W2318756323 endingPage "4382" @default.
• W2318756323 startingPage "4371" @default.
• W2318756323 abstract "Alkaline phosphatase (ALP) of Helicoverpa armigera Hub. (Lepidoptera; Noctuidae) (GenBank accession No. EU729322) was cloned and expressed. The target gene H.a-ALP, having an open reading frame of 1608 bp, was reverse-transcribed from cDNA by the polymerase chain reaction. The open reading frame of the target gene was cloned into the pET-32a expression vector to obtain recombinant protein in Escherichia coli DE-3 cells for the subsequent production of polyclonal antibody. New Zealand white rabbits were used for production of anti-pET-32a-H.a-ALP. The production of antibody was also optimized by employing ELISA for titer determination. The produced antiserum was processed and used as an antibody. Western blot results showed that the polyclonal antibody produced was capable of effectively binding target protein not only from H. armigera but also from other lepidopterans such as Mythimna separata and Plutella xylostella. This antibody was also used to detect levels of ALP within different instars of H. armigera. Thus, it is concluded that this antibody-based assay is very useful for the effective detection of gene-specific expression. Furthermore, it may also be used to detect the expression levels and tissue localization of ALP, as well as in other physiological studies involving this enzyme." @default.
• W2318756323 created "2016-06-24" @default.
• W2318756323 creator A5003168178 @default.
• W2318756323 creator A5045917717 @default.
• W2318756323 creator A5058889592 @default.
• W2318756323 creator A5065065078 @default.
• W2318756323 date "2013-01-01" @default.
• W2318756323 modified "2023-09-29" @default.
• W2318756323 title "Antibody-based detection of alkaline phosphatase in lepidopteran insects (Lepidoptera: Noctuidae)" @default.
• W2318756323 cites W1521916544 @default.
• W2318756323 cites W1968708566 @default.
• W2318756323 cites W1971658398 @default.
• W2318756323 cites W1998318347 @default.
• W2318756323 cites W200986958 @default.
• W2318756323 cites W2014682940 @default.
• W2318756323 cites W2026706138 @default.
• W2318756323 cites W2037411609 @default.
• W2318756323 cites W2038262004 @default.
• W2318756323 cites W2045442440 @default.
• W2318756323 cites W2047990465 @default.
• W2318756323 cites W2060343084 @default.
• W2318756323 cites W2071609652 @default.
• W2318756323 cites W2079254210 @default.
• W2318756323 cites W2079728319 @default.
• W2318756323 cites W2085719132 @default.
• W2318756323 cites W2088419661 @default.
• W2318756323 cites W2116496026 @default.
• W2318756323 cites W2173890278 @default.
• W2318756323 cites W2233884374 @default.
• W2318756323 cites W37545008 @default.
• W2318756323 doi "https://doi.org/10.4238/2013.october.10.3" @default.
• W2318756323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24222217" @default.
• W2318756323 hasPublicationYear "2013" @default.
• W2318756323 type Work @default.
• W2318756323 sameAs 2318756323 @default.
• W2318756323 citedByCount "1" @default.
• W2318756323 countsByYear W23187563232015 @default.
• W2318756323 crossrefType "journal-article" @default.
• W2318756323 hasAuthorship W2318756323A5003168178 @default.
• W2318756323 hasAuthorship W2318756323A5045917717 @default.
• W2318756323 hasAuthorship W2318756323A5058889592 @default.
• W2318756323 hasAuthorship W2318756323A5065065078 @default.
• W2318756323 hasBestOaLocation W23187563231 @default.
• W2318756323 hasConcept C104317684 @default.
• W2318756323 hasConcept C153911025 @default.
• W2318756323 hasConcept C159654299 @default.
• W2318756323 hasConcept C160160445 @default.
• W2318756323 hasConcept C167625842 @default.
• W2318756323 hasConcept C177756618 @default.
• W2318756323 hasConcept C181199279 @default.
• W2318756323 hasConcept C187882448 @default.
• W2318756323 hasConcept C203014093 @default.
• W2318756323 hasConcept C2776273252 @default.
• W2318756323 hasConcept C2776712339 @default.
• W2318756323 hasConcept C2778350275 @default.
• W2318756323 hasConcept C2780157400 @default.
• W2318756323 hasConcept C33161422 @default.
• W2318756323 hasConcept C40767141 @default.
• W2318756323 hasConcept C47289529 @default.
• W2318756323 hasConcept C55493867 @default.
• W2318756323 hasConcept C59822182 @default.
• W2318756323 hasConcept C86803240 @default.
• W2318756323 hasConcept C91523082 @default.
• W2318756323 hasConcept C956191 @default.
• W2318756323 hasConceptScore W2318756323C104317684 @default.
• W2318756323 hasConceptScore W2318756323C153911025 @default.
• W2318756323 hasConceptScore W2318756323C159654299 @default.
• W2318756323 hasConceptScore W2318756323C160160445 @default.
• W2318756323 hasConceptScore W2318756323C167625842 @default.
• W2318756323 hasConceptScore W2318756323C177756618 @default.
• W2318756323 hasConceptScore W2318756323C181199279 @default.
• W2318756323 hasConceptScore W2318756323C187882448 @default.
• W2318756323 hasConceptScore W2318756323C203014093 @default.
• W2318756323 hasConceptScore W2318756323C2776273252 @default.
• W2318756323 hasConceptScore W2318756323C2776712339 @default.
• W2318756323 hasConceptScore W2318756323C2778350275 @default.
• W2318756323 hasConceptScore W2318756323C2780157400 @default.
• W2318756323 hasConceptScore W2318756323C33161422 @default.
• W2318756323 hasConceptScore W2318756323C40767141 @default.
• W2318756323 hasConceptScore W2318756323C47289529 @default.
• W2318756323 hasConceptScore W2318756323C55493867 @default.
• W2318756323 hasConceptScore W2318756323C59822182 @default.
• W2318756323 hasConceptScore W2318756323C86803240 @default.
• W2318756323 hasConceptScore W2318756323C91523082 @default.
• W2318756323 hasConceptScore W2318756323C956191 @default.
• W2318756323 hasIssue "4" @default.
• W2318756323 hasLocation W23187563231 @default.
• W2318756323 hasLocation W23187563232 @default.
• W2318756323 hasOpenAccess W2318756323 @default.
• W2318756323 hasPrimaryLocation W23187563231 @default.
• W2318756323 hasRelatedWork W2318756323 @default.
• W2318756323 hasRelatedWork W2359256335 @default.
• W2318756323 hasRelatedWork W2371168668 @default.
• W2318756323 hasRelatedWork W2384578900 @default.
• W2318756323 hasRelatedWork W2391268768 @default.
• W2318756323 hasRelatedWork W2392446120 @default.
• W2318756323 hasRelatedWork W2394115102 @default.
• W2318756323 hasRelatedWork W2462039668 @default.

• next