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• W2318785356 abstract "Event Abstract Back to Event ROS sensitive collagen fibers with gradient concentration of MnO2 nanoparticles inhibit annulus fibrosus cell apoptosis. Christos Tapeinos1, Akshay Srivastava1, Sahana Ganesh1 and Abhay Pandit1 1 National University of Ireland Galway, Center for Research in Medical Devices, Ireland Introduction: The term inflammation is used to characterize a number of biological processes that take place when natural (tissue injury) or pathogenic (microbes, bacteria) factors attack the human body. Inflammation can occur in any tissue and one of the main characteristics of these inflamed tissues is the low levels of oxygen. The hypoxic microenvironment that is created around these inflamed tissues is one of the reasons responsible for the production of ROS. We hypothesize that collagen fibers with concentration gradients of manganese dioxide (MnO2) nanoparticles (NPs) can increase oxygen levels in a control manner through the reaction of MnO2 NPs with hydrogen peroxide (H2O2) resulting in attenuated hypoxia in inflamed tissues. Hence, our specific objective in this study is to fabricate collagen fibers embedded with MnO2 NPs (Fig. 1), with controlled degradability that are able to scavenge H2O2 and generate O2 to inhibit Annulus Fibrosus (AF) cell apoptosis under inflamed condition. Materials and Methods: MnO2 NPs were synthesized using a previously described method[1],[2]. The NPs were characterized structurally (XRD & IR) and morphologically (TEM) and their charge was measured with DLS. The same characterizations applied also on the fibers embedded with the NPs. An oxygen sensor was used to measure the increase in oxygen levels after the reaction of the NPs with various H2O2 concentrations. The NPs were mixed with Type I bovine collagen (3.5 mg/ml) in various concentrations and fibers were synthesized by the fiber extrusion method (Fig. 1A). The degradation profile as well as oxygen levels after reaction with H2O2 were assessed. Cytotoxicity of the NPs and of the fibers embedded with NPs was carried out on 3T3 fibroblast and IL-1β induced inflamed AF cells (Fig. 2D). Changes in the apoptosis rate and intracellular ROS levels in cultured inflamed AF cells were measured by flow cytometry. Results and Discussion: MnO2 NPs were successfully fabricated and embedded inside the collagen fibers in different concentrations creating a gradient along the fibers. Oxygen was generated following the reaction of the NPs with H2O2 (Fig. 2B) and the increase in oxygen was found to be proportional to the nanoparticles’ concentration. In vitro studies on 3T3 fibroblast cells after treatment with H2O2 before and after treatment with MnO2 NPs demonstrated that cells’ metabolic activity is higher after reaction with MnO2, indicating the scavenging effect of the NPs (Fig. 2C). Flow cytometry results showed that MnO2 loaded collagen fibers exhibit increased cell viability, decreased ROS formation and significantly reduced apoptosis. Conclusions: Modified collagen-MnO2 fibers with controlled degradation rates were successfully fabricated. These fibers were able to generate oxygen after treatment with various concentrations of H2O2. In vitro studies demonstrated a subsequent reduction in ROS levels and inflamed AF cell apoptosis after treatment with the modified fibers. Future studieds will involve measuring altered mitochondrial membrane potential of AF cells and molecular markers involved in extracellular matrix production. This publication has emanated from research supported in part by a research grant from Science Foundation Ireland (SFI) and is co-funded under the European Regional Development Fund under Grant Number 13/RC/2073; This material is based upon works supported by the European Union funding under the 7th Framework Programme under Grant Agreement Number 317304References:[1] Y. Luo, Materials Letters, 2007, 61, 1893-1895[2] P. Prasad, C. R. Gordijo, A. Z. Abbasi, A. Maeda, A. Ip, A. M. Rauth, R. S. DaCosta and X. Y. Wu, ACS Nano, 2014, 8, 3202-3212 Keywords: biomaterial, stimuli-response, Biodegradable material, Nano/micro particle Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: General Session Oral Topic: Role of biomaterials in inflammation Citation: Tapeinos C, Srivastava A, Ganesh S and Pandit A (2016). ROS sensitive collagen fibers with gradient concentration of MnO2 nanoparticles inhibit annulus fibrosus cell apoptosis.. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02303 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Christos Tapeinos Akshay Srivastava Sahana Ganesh Abhay Pandit Google Christos Tapeinos Akshay Srivastava Sahana Ganesh Abhay Pandit Google Scholar Christos Tapeinos Akshay Srivastava Sahana Ganesh Abhay Pandit PubMed Christos Tapeinos Akshay Srivastava Sahana Ganesh Abhay Pandit Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W2318785356 title "ROS sensitive collagen fibers with gradient concentration of MnO2 nanoparticles inhibit annulus fibrosus cell apoptosis." @default.
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