FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2319173652> ?p ?o ?g. }

• W2319173652 abstract "Background and objectives SMOC2, a secreted calcium-binding protein of the BM-40/SPARC family was identified from a chondrogenic extract of articular cartilage and is increased in osteoarthritic cartilage. We evaluated the impact of SMOC2 on in vitro chondrogenesis and on in vitro osteogenesis and mineralization. Materials and methods Smoc2 was stably overexpressed (Smoc2+) or silenced (Smoc2-). We also stably overexpressed Smoc2 lacking the Calcium-binding domain (ΔCaBD). ATDC5 cells were cultured as micromasses. Gene expression of chondrogenic genes (Acan, Col2a1, Col10a1), Wnt- and BMP genes was analysed by RT-qPCR. Safranin O, Sirius red, Alcian Blue and Alizarin red staining were performed to evaluate collagens, proteoglycans and mineralization. MC3T3 cells were cultured in monolayer. Alkaline phosphatase activity (ALP) was measured and mineralization was analysed by alizarin red staining. Gene expression of extracellular matrix genes, genes linked to mineralization and TGFβ superfamily genes was analysed by RT-qPCR. Intracellular signalling pathways (PKCα, ERK1/2, SMAD1/5/8, p38MAPK) were investigated by Western blot. Data were translationally validated in human periosteal and endothelial cells. Results Mineralization and ALP activity was reduced in Smoc2+ MC3T3 cells. Gene expression analysis showed an overall altered differentiation when overexpressing Smoc2. The activation status of PKCα, ERK1/2, p38MAPK and SMAD1/5/8 was significantly modified in Smoc2+ cells. We could not observe an effect on osteogenesis when silencing Smoc2. ΔCaBD cells however exhibited less of the inhibiting effects of Smoc2+ cells. Moreover we could observe partial restoration after addition of extracellular calcium to the culture medium of differentiating Smoc2+ cells. Alizarin red staining and ALP activity was reduced in hPDCs cultured in the presence of Smoc2+ supernatant compared to controls. However, hPDCs stimulated with ΔCaBD supernatants exhibited a lesser decrease of these markers compared to Smoc2+. Alizarin red quantification showed the same effect for HUVECs. In ATDC5 cells, Smoc2 overexpression altered chondrogenic markers and reduced Wnt and BMP signalling. Smoc2 silencing enhanced chondrogenic differentiation by increasing BMP signalling. Conclusions SMOC2 can regulate osteogenesis and chondrogenesis. Silencing of Smoc2 did not affect osteogenesis suggesting a limited endogenous role. SMOC2 appears to exert its effects through interaction with calcium and by interfering with BMP and Wnt signalling." @default.
• W2319173652 created "2016-06-24" @default.
• W2319173652 creator A5018912842 @default.
• W2319173652 creator A5053030525 @default.
• W2319173652 creator A5089333285 @default.
• W2319173652 date "2016-02-01" @default.
• W2319173652 modified "2023-09-26" @default.
• W2319173652 title "A4.05 SMOC2, a secreted calcium-binding protein from cartilage extracellular matrix is an inhibitor of cartilage and bone formation" @default.
• W2319173652 doi "https://doi.org/10.1136/annrheumdis-2016-209124.93" @default.
• W2319173652 hasPublicationYear "2016" @default.
• W2319173652 type Work @default.
• W2319173652 sameAs 2319173652 @default.
• W2319173652 citedByCount "0" @default.
• W2319173652 crossrefType "journal-article" @default.
• W2319173652 hasAuthorship W2319173652A5018912842 @default.
• W2319173652 hasAuthorship W2319173652A5053030525 @default.
• W2319173652 hasAuthorship W2319173652A5089333285 @default.
• W2319173652 hasConcept C105702510 @default.
• W2319173652 hasConcept C153911025 @default.
• W2319173652 hasConcept C185592680 @default.
• W2319173652 hasConcept C189165786 @default.
• W2319173652 hasConcept C2780550940 @default.
• W2319173652 hasConcept C28406088 @default.
• W2319173652 hasConcept C35496256 @default.
• W2319173652 hasConcept C55493867 @default.
• W2319173652 hasConcept C86803240 @default.
• W2319173652 hasConcept C95444343 @default.
• W2319173652 hasConceptScore W2319173652C105702510 @default.
• W2319173652 hasConceptScore W2319173652C153911025 @default.
• W2319173652 hasConceptScore W2319173652C185592680 @default.
• W2319173652 hasConceptScore W2319173652C189165786 @default.
• W2319173652 hasConceptScore W2319173652C2780550940 @default.
• W2319173652 hasConceptScore W2319173652C28406088 @default.
• W2319173652 hasConceptScore W2319173652C35496256 @default.
• W2319173652 hasConceptScore W2319173652C55493867 @default.
• W2319173652 hasConceptScore W2319173652C86803240 @default.
• W2319173652 hasConceptScore W2319173652C95444343 @default.
• W2319173652 hasLocation W23191736521 @default.
• W2319173652 hasOpenAccess W2319173652 @default.
• W2319173652 hasPrimaryLocation W23191736521 @default.
• W2319173652 hasRelatedWork W1971617450 @default.
• W2319173652 hasRelatedWork W1979549276 @default.
• W2319173652 hasRelatedWork W2046923971 @default.
• W2319173652 hasRelatedWork W2067815209 @default.
• W2319173652 hasRelatedWork W2085251589 @default.
• W2319173652 hasRelatedWork W2415644792 @default.
• W2319173652 hasRelatedWork W2534423616 @default.
• W2319173652 hasRelatedWork W2604323310 @default.
• W2319173652 hasRelatedWork W2744918394 @default.
• W2319173652 hasRelatedWork W2789356248 @default.
• W2319173652 hasRelatedWork W2808137767 @default.
• W2319173652 hasRelatedWork W2956033661 @default.
• W2319173652 hasRelatedWork W3029136858 @default.
• W2319173652 hasRelatedWork W3030124164 @default.
• W2319173652 hasRelatedWork W3030889806 @default.
• W2319173652 hasRelatedWork W3031315870 @default.
• W2319173652 hasRelatedWork W3045626901 @default.
• W2319173652 hasRelatedWork W3119892325 @default.
• W2319173652 hasRelatedWork W3201880945 @default.
• W2319173652 hasRelatedWork W2579549553 @default.
• W2319173652 isParatext "false" @default.
• W2319173652 isRetracted "false" @default.
• W2319173652 magId "2319173652" @default.
• W2319173652 workType "article" @default.