Matches in SemOpenAlex for { <https://semopenalex.org/work/W2319239696> ?p ?o ?g. }
Showing items 1 to 89 of
89
with 100 items per page.
- W2319239696 endingPage "2713" @default.
- W2319239696 startingPage "2710" @default.
- W2319239696 abstract "Beside cooling/warming rates and composition of vitrification solution, developmental stage of immature oocytes may also affect their vitrification outcome. The aim of the present study was to evaluate the selection effect of developmentally competent immature bovine oocytes by Brilliant Cresyl Blue (BCB) on maturity of oocytes after vitrification. Oocytes were obtained from slaughterhouse ovaries. Only oocytes with 4-5 layers of cumulus cells and homogenous cytoplasm were used. After exposure to BCB stain, immature oocytes were divided into colored (BCB+) and colorless (BCB-) cytoplasm groups. Immature oocytes were equilibrated in VS1 (7.5 Ethylene Glycol (EG)+7.5% DMSO) for 10-12 min and then exposed to VS2 (15% EG+ 15% DMSO+0.5M sucrose) for 1 min. Thereafter, oocytes were loaded on Cryotop and directly plunged into liquid nitrogen. After warming, oocytes were examined for presence of polar body and nuclear maturity. Higher number of oocytes in BCB+group extruded first polar body in comparison with other vitrified groups but not significantly (p>0.05). Compared to the BCB- oocytes, there was significantly lower percentage of degeneration for BCB+oocytes (p<0.05). Within vitrified groups, reaching to the MII stage was significantly higher in BCB+group (51.5%) compared with BCB and vitrified-control groups (27.9 and 40.3%, respectively). These results indicated that selection of potent immature bovine oocytes using brilliant cresyl blue improved the nuclear maturity of immature oocytes after vitrification. In addition, this selection can be a valuable tool to improve the vitrification outcome." @default.
- W2319239696 created "2016-06-24" @default.
- W2319239696 creator A5007564052 @default.
- W2319239696 creator A5017217963 @default.
- W2319239696 creator A5026907792 @default.
- W2319239696 creator A5039499453 @default.
- W2319239696 creator A5056750678 @default.
- W2319239696 creator A5067534636 @default.
- W2319239696 creator A5072411516 @default.
- W2319239696 creator A5072990164 @default.
- W2319239696 creator A5088208147 @default.
- W2319239696 date "2010-12-01" @default.
- W2319239696 modified "2023-10-18" @default.
- W2319239696 title "Selection of Immature Bovine Oocytes Using Brilliant Cresyl Blue Enhances Nuclear Maturity after Vitrification" @default.
- W2319239696 cites W1966405565 @default.
- W2319239696 cites W1974735468 @default.
- W2319239696 cites W1995462934 @default.
- W2319239696 cites W2006731805 @default.
- W2319239696 cites W2019918299 @default.
- W2319239696 cites W2023591091 @default.
- W2319239696 cites W2041019830 @default.
- W2319239696 cites W2050587143 @default.
- W2319239696 cites W2063745332 @default.
- W2319239696 cites W2077098848 @default.
- W2319239696 cites W2079238504 @default.
- W2319239696 cites W2085886258 @default.
- W2319239696 cites W2108512070 @default.
- W2319239696 cites W2110735000 @default.
- W2319239696 cites W2118401305 @default.
- W2319239696 cites W2121151812 @default.
- W2319239696 cites W2159096946 @default.
- W2319239696 cites W2167152913 @default.
- W2319239696 doi "https://doi.org/10.3923/javaa.2010.2710.2713" @default.
- W2319239696 hasPublicationYear "2010" @default.
- W2319239696 type Work @default.
- W2319239696 sameAs 2319239696 @default.
- W2319239696 citedByCount "6" @default.
- W2319239696 countsByYear W23192396962013 @default.
- W2319239696 countsByYear W23192396962016 @default.
- W2319239696 countsByYear W23192396962019 @default.
- W2319239696 countsByYear W23192396962022 @default.
- W2319239696 countsByYear W23192396962023 @default.
- W2319239696 crossrefType "journal-article" @default.
- W2319239696 hasAuthorship W2319239696A5007564052 @default.
- W2319239696 hasAuthorship W2319239696A5017217963 @default.
- W2319239696 hasAuthorship W2319239696A5026907792 @default.
- W2319239696 hasAuthorship W2319239696A5039499453 @default.
- W2319239696 hasAuthorship W2319239696A5056750678 @default.
- W2319239696 hasAuthorship W2319239696A5067534636 @default.
- W2319239696 hasAuthorship W2319239696A5072411516 @default.
- W2319239696 hasAuthorship W2319239696A5072990164 @default.
- W2319239696 hasAuthorship W2319239696A5088208147 @default.
- W2319239696 hasBestOaLocation W23192396961 @default.
- W2319239696 hasConcept C154945302 @default.
- W2319239696 hasConcept C16685009 @default.
- W2319239696 hasConcept C2778022349 @default.
- W2319239696 hasConcept C41008148 @default.
- W2319239696 hasConcept C71924100 @default.
- W2319239696 hasConcept C81917197 @default.
- W2319239696 hasConcept C86803240 @default.
- W2319239696 hasConceptScore W2319239696C154945302 @default.
- W2319239696 hasConceptScore W2319239696C16685009 @default.
- W2319239696 hasConceptScore W2319239696C2778022349 @default.
- W2319239696 hasConceptScore W2319239696C41008148 @default.
- W2319239696 hasConceptScore W2319239696C71924100 @default.
- W2319239696 hasConceptScore W2319239696C81917197 @default.
- W2319239696 hasConceptScore W2319239696C86803240 @default.
- W2319239696 hasIssue "21" @default.
- W2319239696 hasLocation W23192396961 @default.
- W2319239696 hasLocation W23192396962 @default.
- W2319239696 hasOpenAccess W2319239696 @default.
- W2319239696 hasPrimaryLocation W23192396961 @default.
- W2319239696 hasRelatedWork W1202469404 @default.
- W2319239696 hasRelatedWork W17480179 @default.
- W2319239696 hasRelatedWork W2001959059 @default.
- W2319239696 hasRelatedWork W2023558490 @default.
- W2319239696 hasRelatedWork W2027998578 @default.
- W2319239696 hasRelatedWork W2067577249 @default.
- W2319239696 hasRelatedWork W2093666188 @default.
- W2319239696 hasRelatedWork W2132619831 @default.
- W2319239696 hasRelatedWork W2150487831 @default.
- W2319239696 hasRelatedWork W3207358282 @default.
- W2319239696 hasVolume "9" @default.
- W2319239696 isParatext "false" @default.
- W2319239696 isRetracted "false" @default.
- W2319239696 magId "2319239696" @default.
- W2319239696 workType "article" @default.