Matches in SemOpenAlex for { <https://semopenalex.org/work/W2319315919> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2319315919 endingPage "111" @default.
- W2319315919 startingPage "105" @default.
- W2319315919 abstract "Structural investigations need ready supply of the isotope labeled proteins with inserted mutations n the quantities sufficient for the heteronuclear NMR. Though cell-free expression system has been widely used in the past years, high startup cost and complex compound composition prevent many researches from the developing this technique, especially for membrane protein production. Here we demonstrate the utility of a robust, cost-optimized cell-free expression technique for production of the physiologically important transmembrane fragment of amyloid precursor protein, APP686-726, containing Alzheimer's disease mutations in the juxtamembrane (E693G, Arctic form) and the transmembrane parts (V717G, London form, or L723P, Australian form). The protein cost was optimized by varying the FM/RM ratio as well as the amino acid concentration. We obtained the wild-type and mutant transmembrane fragments in the pellet mode of continuous exchange cell-free system consuming only commercial algal mixture of the 13C,15N-labeled amino acids. Scaling up analytical tests, we achieved milligram quantity yields of isotope labeled wild-type and mutant APP686-726 for structural studies by high resolution NMR spectroscopy in membrane mimicking environment. The described approach has from 5 to 23-fold cost advantage over the bacterial expression methods described earlier and 1.5 times exceeds our previous result obtained with the longer APP671-726WT fragment." @default.
- W2319315919 created "2016-06-24" @default.
- W2319315919 creator A5007754320 @default.
- W2319315919 creator A5008332261 @default.
- W2319315919 creator A5012872518 @default.
- W2319315919 creator A5055846192 @default.
- W2319315919 creator A5068140693 @default.
- W2319315919 date "2016-07-01" @default.
- W2319315919 modified "2023-10-07" @default.
- W2319315919 title "Cell-free expression of the APP transmembrane fragments with Alzheimer's disease mutations using algal amino acid mixture for structural NMR studies" @default.
- W2319315919 cites W1936146647 @default.
- W2319315919 cites W1964305847 @default.
- W2319315919 cites W2003238246 @default.
- W2319315919 cites W2009077514 @default.
- W2319315919 cites W2011201961 @default.
- W2319315919 cites W2019677904 @default.
- W2319315919 cites W2027241185 @default.
- W2319315919 cites W2031807129 @default.
- W2319315919 cites W2042537143 @default.
- W2319315919 cites W2050586736 @default.
- W2319315919 cites W2060802768 @default.
- W2319315919 cites W2062876104 @default.
- W2319315919 cites W2066958493 @default.
- W2319315919 cites W2078534574 @default.
- W2319315919 cites W2081821620 @default.
- W2319315919 cites W2088945858 @default.
- W2319315919 cites W2090930620 @default.
- W2319315919 cites W2151128898 @default.
- W2319315919 cites W2166025323 @default.
- W2319315919 cites W2166151730 @default.
- W2319315919 cites W304622482 @default.
- W2319315919 cites W4322701865 @default.
- W2319315919 cites W53300240 @default.
- W2319315919 doi "https://doi.org/10.1016/j.pep.2016.04.004" @default.
- W2319315919 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27071311" @default.
- W2319315919 hasPublicationYear "2016" @default.
- W2319315919 type Work @default.
- W2319315919 sameAs 2319315919 @default.
- W2319315919 citedByCount "10" @default.
- W2319315919 countsByYear W23193159192017 @default.
- W2319315919 countsByYear W23193159192018 @default.
- W2319315919 countsByYear W23193159192019 @default.
- W2319315919 countsByYear W23193159192020 @default.
- W2319315919 countsByYear W23193159192021 @default.
- W2319315919 countsByYear W23193159192023 @default.
- W2319315919 crossrefType "journal-article" @default.
- W2319315919 hasAuthorship W2319315919A5007754320 @default.
- W2319315919 hasAuthorship W2319315919A5008332261 @default.
- W2319315919 hasAuthorship W2319315919A5012872518 @default.
- W2319315919 hasAuthorship W2319315919A5055846192 @default.
- W2319315919 hasAuthorship W2319315919A5068140693 @default.
- W2319315919 hasConcept C104317684 @default.
- W2319315919 hasConcept C118892022 @default.
- W2319315919 hasConcept C143065580 @default.
- W2319315919 hasConcept C144647389 @default.
- W2319315919 hasConcept C170493617 @default.
- W2319315919 hasConcept C185592680 @default.
- W2319315919 hasConcept C24530287 @default.
- W2319315919 hasConcept C2778665413 @default.
- W2319315919 hasConcept C3675279 @default.
- W2319315919 hasConcept C41625074 @default.
- W2319315919 hasConcept C515207424 @default.
- W2319315919 hasConcept C55493867 @default.
- W2319315919 hasConcept C86803240 @default.
- W2319315919 hasConceptScore W2319315919C104317684 @default.
- W2319315919 hasConceptScore W2319315919C118892022 @default.
- W2319315919 hasConceptScore W2319315919C143065580 @default.
- W2319315919 hasConceptScore W2319315919C144647389 @default.
- W2319315919 hasConceptScore W2319315919C170493617 @default.
- W2319315919 hasConceptScore W2319315919C185592680 @default.
- W2319315919 hasConceptScore W2319315919C24530287 @default.
- W2319315919 hasConceptScore W2319315919C2778665413 @default.
- W2319315919 hasConceptScore W2319315919C3675279 @default.
- W2319315919 hasConceptScore W2319315919C41625074 @default.
- W2319315919 hasConceptScore W2319315919C515207424 @default.
- W2319315919 hasConceptScore W2319315919C55493867 @default.
- W2319315919 hasConceptScore W2319315919C86803240 @default.
- W2319315919 hasFunder F4320321079 @default.
- W2319315919 hasFunder F4320334801 @default.
- W2319315919 hasLocation W23193159191 @default.
- W2319315919 hasLocation W23193159192 @default.
- W2319315919 hasOpenAccess W2319315919 @default.
- W2319315919 hasPrimaryLocation W23193159191 @default.
- W2319315919 hasRelatedWork W1424623456 @default.
- W2319315919 hasRelatedWork W1974240269 @default.
- W2319315919 hasRelatedWork W1992657771 @default.
- W2319315919 hasRelatedWork W2040208869 @default.
- W2319315919 hasRelatedWork W2045836921 @default.
- W2319315919 hasRelatedWork W2071368711 @default.
- W2319315919 hasRelatedWork W2097747021 @default.
- W2319315919 hasRelatedWork W2157535945 @default.
- W2319315919 hasRelatedWork W2315040272 @default.
- W2319315919 hasRelatedWork W2319315919 @default.
- W2319315919 hasVolume "123" @default.
- W2319315919 isParatext "false" @default.
- W2319315919 isRetracted "false" @default.
- W2319315919 magId "2319315919" @default.
- W2319315919 workType "article" @default.