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- W2319319273 abstract "Hop stunt viroid (HSV), cucumber pale fruit viroid (CPFV), and potato spindle tuber viroid (PSTV) were co-electrophoresed in polyacrylamide gel (PAGE) under both non-denaturing and denaturing conditions. On our experimental condition, they migrated at almost the same rate in 15% PAGE of Tris-acetate-EDTA buffer (non-denaturing condition). Under the 7M urea-denaturing condition, the viroids migrated differently in 5% or 7.5% PAGE of Tris-borate-EDTA buffer. The circular form of HSV and CPFV migrated apparently faster than that of PSTV. The circular form of HSV migrated slightly faster than that of CPFV. A linear form of HSV and CPFV were not detected by staining of the gel but its presence was indicated by infectivity assay. It was difficult to distinguish HSV and CPFV by electron microscopic examination. Mean length of HSV-and CPFV-circular molecules were 83±2.4nm and 82±4.2nm, respectively. The linear form was scarcely observed in both HSV and CPFV preparations." @default.
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- W2319319273 date "1984-01-01" @default.
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- W2319319273 title "Comparative studies of hop stunt viroid and cucumber pale fruit viroid by polyacrylamide gel electrophoretic analysis and electron microscopic examination." @default.
- W2319319273 doi "https://doi.org/10.3186/jjphytopath.50.339" @default.
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