FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2319438855> ?p ?o ?g. }

• W2319438855 endingPage "41" @default.
• W2319438855 startingPage "41" @default.
• W2319438855 abstract "Rapid activation of innate immune defences upon microbial infection depends on the evolutionary conserved NF-κB dependent signals which deregulation is frequently associated with chronic inflammation and oncogenesis. These signals are tightly regulated by the linkage of different kinds of ubiquitin moieties on proteins that modify either their activity or their stability. To investigate how ubiquitin specific proteases (USPs) orchestrate immune signal regulation, we created and screened a focused RNA interference library on Drosophila NF-κB-like pathways Toll and Imd in cultured S2 cells, and further analysed the function of selected genes in vivo. We report here that USP2 and USP34/Puf, in addition to the previously described USP36/Scny, prevent inappropriate activation of Imd-dependent immune signal in unchallenged conditions. Moreover, USP34 is also necessary to prevent constitutive activation of the Toll pathway. However, while USP2 also prevents excessive Imd-dependent signalling in vivo, USP34 shows differential requirement depending on NF-κB target genes, in response to fly infection by either Gram-positive or Gram-negative bacteria. We further show that USP2 prevents the constitutive activation of signalling by promoting Imd proteasomal degradation. Indeed, the homeostasis of the Imd scaffolding molecule is tightly regulated by the linkage of lysine 48-linked ubiquitin chains (K48) acting as a tag for its proteasomal degradation. This process is necessary to prevent constitutive activation of Imd pathway in vivo and is inhibited in response to infection. The control of Imd homeostasis by USP2 is associated with the hydrolysis of Imd linked K48-ubiquitin chains and the synergistic binding of USP2 and Imd to the proteasome, as evidenced by both mass-spectrometry analysis of USP2 partners and by co-immunoprecipitation experiments. Our work identified one known (USP36) and two new (USP2, USP34) ubiquitin specific proteases regulating Imd or Toll dependent immune signalling in Drosophila. It further highlights the ubiquitin dependent control of Imd homeostasis and shows a new activity for USP2 at the proteasome allowing for Imd degradation. This study provides original information for the better understanding of the strong implication of USP2 in pathological processes in humans, including cancerogenesis." @default.
• W2319438855 created "2016-06-24" @default.
• W2319438855 creator A5015117076 @default.
• W2319438855 creator A5025367066 @default.
• W2319438855 creator A5053693692 @default.
• W2319438855 creator A5060431275 @default.
• W2319438855 creator A5072074508 @default.
• W2319438855 creator A5072577973 @default.
• W2319438855 creator A5091639519 @default.
• W2319438855 date "2014-01-01" @default.
• W2319438855 modified "2023-10-18" @default.
• W2319438855 title "Identifying USPs regulating immune signals in" @default.
• W2319438855 doi "https://doi.org/10.1186/preaccept-1588328929121802" @default.
• W2319438855 hasPublicationYear "2014" @default.
• W2319438855 type Work @default.
• W2319438855 sameAs 2319438855 @default.
• W2319438855 citedByCount "0" @default.
• W2319438855 crossrefType "journal-article" @default.
• W2319438855 hasAuthorship W2319438855A5015117076 @default.
• W2319438855 hasAuthorship W2319438855A5025367066 @default.
• W2319438855 hasAuthorship W2319438855A5053693692 @default.
• W2319438855 hasAuthorship W2319438855A5060431275 @default.
• W2319438855 hasAuthorship W2319438855A5072074508 @default.
• W2319438855 hasAuthorship W2319438855A5072577973 @default.
• W2319438855 hasAuthorship W2319438855A5091639519 @default.
• W2319438855 hasBestOaLocation W23194388551 @default.
• W2319438855 hasConcept C104317684 @default.
• W2319438855 hasConcept C136449434 @default.
• W2319438855 hasConcept C181199279 @default.
• W2319438855 hasConcept C185592680 @default.
• W2319438855 hasConcept C203014093 @default.
• W2319438855 hasConcept C207001950 @default.
• W2319438855 hasConcept C25602115 @default.
• W2319438855 hasConcept C2776914184 @default.
• W2319438855 hasConcept C2781307694 @default.
• W2319438855 hasConcept C54355233 @default.
• W2319438855 hasConcept C55493867 @default.
• W2319438855 hasConcept C62478195 @default.
• W2319438855 hasConcept C86803240 @default.
• W2319438855 hasConcept C8891405 @default.
• W2319438855 hasConcept C95444343 @default.
• W2319438855 hasConceptScore W2319438855C104317684 @default.
• W2319438855 hasConceptScore W2319438855C136449434 @default.
• W2319438855 hasConceptScore W2319438855C181199279 @default.
• W2319438855 hasConceptScore W2319438855C185592680 @default.
• W2319438855 hasConceptScore W2319438855C203014093 @default.
• W2319438855 hasConceptScore W2319438855C207001950 @default.
• W2319438855 hasConceptScore W2319438855C25602115 @default.
• W2319438855 hasConceptScore W2319438855C2776914184 @default.
• W2319438855 hasConceptScore W2319438855C2781307694 @default.
• W2319438855 hasConceptScore W2319438855C54355233 @default.
• W2319438855 hasConceptScore W2319438855C55493867 @default.
• W2319438855 hasConceptScore W2319438855C62478195 @default.
• W2319438855 hasConceptScore W2319438855C86803240 @default.
• W2319438855 hasConceptScore W2319438855C8891405 @default.
• W2319438855 hasConceptScore W2319438855C95444343 @default.
• W2319438855 hasIssue "1" @default.
• W2319438855 hasLocation W23194388551 @default.
• W2319438855 hasLocation W23194388552 @default.
• W2319438855 hasLocation W23194388553 @default.
• W2319438855 hasLocation W23194388554 @default.
• W2319438855 hasLocation W23194388555 @default.
• W2319438855 hasOpenAccess W2319438855 @default.
• W2319438855 hasPrimaryLocation W23194388551 @default.
• W2319438855 hasRelatedWork W1967175706 @default.
• W2319438855 hasRelatedWork W1981947243 @default.
• W2319438855 hasRelatedWork W2090890815 @default.
• W2319438855 hasRelatedWork W2145019698 @default.
• W2319438855 hasRelatedWork W2182498414 @default.
• W2319438855 hasRelatedWork W2531302392 @default.
• W2319438855 hasRelatedWork W2939067693 @default.
• W2319438855 hasRelatedWork W3095351377 @default.
• W2319438855 hasRelatedWork W3214245850 @default.
• W2319438855 hasRelatedWork W4211095228 @default.
• W2319438855 hasVolume "12" @default.
• W2319438855 isParatext "false" @default.
• W2319438855 isRetracted "false" @default.
• W2319438855 magId "2319438855" @default.
• W2319438855 workType "article" @default.