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- W2319490282 abstract "When phospholipase A1 (PLA1) was bio-imprinted with 5% soybean lecithin at pH 5.0, its phospholipase (PLA) activity in lecithin–hexane solution increased by 30.9-fold and its substrate specificity was substantially enhanced. A further increase in PLA activity of the bio-imprinted PLA1 (bi-PLA1) from 562 U/g to 1288 U/g was obtained when 105 mg diatomite/mg bi-PLA1 was incorporated as an immobilization carrier. The degumming abilities of PLA1, bi-PLA1, and immobilized bi-PLA1 (im-bi-PLA1) in crude soybean oil and crude oil–hexane (1:1 w/w) miscella were also investigated. The phosphorus content in the crude soybean oil was reduced to 7.3 mg/kg from an initial level of 406.9 mg/kg after incubation with 100 mg/kg im-bi-PLA1 and 2% water at 50 °C for 180 min. In miscella degumming, the residual phosphorus content was reduced from 406.9 mg/kg to 18.7 mg/kg by incubation with 100 mg/kg im-bi-PLA1 and 2% water at 50 °C for 180 min and neutralization with 3 M NaOH solution. In comparison, the original PLA1 reduced the residual phosphorus content to 62.9 mg/kg and 92.4 mg/kg in crude oil and miscella degumming, respectively." @default.
- W2319490282 created "2016-06-24" @default.
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- W2319490282 date "2016-01-01" @default.
- W2319490282 modified "2023-09-22" @default.
- W2319490282 title "Enhancing the performance of a phospholipase A1 for oil degumming by bio-imprinting and immobilization" @default.
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- W2319490282 doi "https://doi.org/10.1016/j.molcatb.2015.11.018" @default.
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