Matches in SemOpenAlex for { <https://semopenalex.org/work/W2319690804> ?p ?o ?g. }
- W2319690804 endingPage "1129" @default.
- W2319690804 startingPage "1123" @default.
- W2319690804 abstract "In this research, a novel method for relative fluorescent quantification of DNA based on Fe3O4@SiO2@Au gold-coated magnetic nanocomposites (GMNPs) and multiplex ligation- dependent probe amplification (MLPA) has been developed. With the help of self-assembly, seed-mediated growth and chemical reduction method, core-shell Fe3O4@SiO2@Au GMNPs were synthesized. Through modified streptavidin on the GMNPs surface, we obtained a bead chip which can capture the biotinylated probes. Then we designed MLPA probes which were tagged with biotin or Cy3 and target DNA on the basis of human APP gene sequence. The products from the thermostable DNA ligase induced ligation reactions and PCR amplifications were incubated with SA-GMNPs. After washing, magnetic separation, spotting, the fluorescent scanning results showed our method can be used for the relative quantitative analysis of the target DNA in the concentration range of 03004~0.5 µM." @default.
- W2319690804 created "2016-06-24" @default.
- W2319690804 creator A5001739227 @default.
- W2319690804 creator A5009544572 @default.
- W2319690804 creator A5019056174 @default.
- W2319690804 creator A5027383886 @default.
- W2319690804 creator A5034410930 @default.
- W2319690804 creator A5048667139 @default.
- W2319690804 creator A5059412519 @default.
- W2319690804 creator A5061453560 @default.
- W2319690804 creator A5074590039 @default.
- W2319690804 creator A5081615766 @default.
- W2319690804 creator A5090346090 @default.
- W2319690804 date "2015-02-01" @default.
- W2319690804 modified "2023-10-14" @default.
- W2319690804 title "Fluorescent Quantification of DNA Based on Core–Shell Fe<SUB>3</SUB>O<SUB>4</SUB>@SiO<SUB>2</SUB>@Au Nanocomposites and Multiplex Ligation-Dependent Probe Amplification" @default.
- W2319690804 doi "https://doi.org/10.1166/jnn.2015.8910" @default.
- W2319690804 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26353621" @default.
- W2319690804 hasPublicationYear "2015" @default.
- W2319690804 type Work @default.
- W2319690804 sameAs 2319690804 @default.
- W2319690804 citedByCount "10" @default.
- W2319690804 countsByYear W23196908042015 @default.
- W2319690804 countsByYear W23196908042016 @default.
- W2319690804 countsByYear W23196908042018 @default.
- W2319690804 countsByYear W23196908042021 @default.
- W2319690804 crossrefType "journal-article" @default.
- W2319690804 hasAuthorship W2319690804A5001739227 @default.
- W2319690804 hasAuthorship W2319690804A5009544572 @default.
- W2319690804 hasAuthorship W2319690804A5019056174 @default.
- W2319690804 hasAuthorship W2319690804A5027383886 @default.
- W2319690804 hasAuthorship W2319690804A5034410930 @default.
- W2319690804 hasAuthorship W2319690804A5048667139 @default.
- W2319690804 hasAuthorship W2319690804A5059412519 @default.
- W2319690804 hasAuthorship W2319690804A5061453560 @default.
- W2319690804 hasAuthorship W2319690804A5074590039 @default.
- W2319690804 hasAuthorship W2319690804A5081615766 @default.
- W2319690804 hasAuthorship W2319690804A5090346090 @default.
- W2319690804 hasConcept C104317684 @default.
- W2319690804 hasConcept C113196181 @default.
- W2319690804 hasConcept C120599132 @default.
- W2319690804 hasConcept C121332964 @default.
- W2319690804 hasConcept C12554922 @default.
- W2319690804 hasConcept C129312508 @default.
- W2319690804 hasConcept C153911025 @default.
- W2319690804 hasConcept C175114707 @default.
- W2319690804 hasConcept C185592680 @default.
- W2319690804 hasConcept C192562407 @default.
- W2319690804 hasConcept C204628709 @default.
- W2319690804 hasConcept C2776190903 @default.
- W2319690804 hasConcept C2778204606 @default.
- W2319690804 hasConcept C2781188995 @default.
- W2319690804 hasConcept C36823959 @default.
- W2319690804 hasConcept C43617362 @default.
- W2319690804 hasConcept C47558249 @default.
- W2319690804 hasConcept C49105822 @default.
- W2319690804 hasConcept C54355233 @default.
- W2319690804 hasConcept C552990157 @default.
- W2319690804 hasConcept C55493867 @default.
- W2319690804 hasConcept C62520636 @default.
- W2319690804 hasConcept C86803240 @default.
- W2319690804 hasConcept C90583042 @default.
- W2319690804 hasConcept C91881484 @default.
- W2319690804 hasConceptScore W2319690804C104317684 @default.
- W2319690804 hasConceptScore W2319690804C113196181 @default.
- W2319690804 hasConceptScore W2319690804C120599132 @default.
- W2319690804 hasConceptScore W2319690804C121332964 @default.
- W2319690804 hasConceptScore W2319690804C12554922 @default.
- W2319690804 hasConceptScore W2319690804C129312508 @default.
- W2319690804 hasConceptScore W2319690804C153911025 @default.
- W2319690804 hasConceptScore W2319690804C175114707 @default.
- W2319690804 hasConceptScore W2319690804C185592680 @default.
- W2319690804 hasConceptScore W2319690804C192562407 @default.
- W2319690804 hasConceptScore W2319690804C204628709 @default.
- W2319690804 hasConceptScore W2319690804C2776190903 @default.
- W2319690804 hasConceptScore W2319690804C2778204606 @default.
- W2319690804 hasConceptScore W2319690804C2781188995 @default.
- W2319690804 hasConceptScore W2319690804C36823959 @default.
- W2319690804 hasConceptScore W2319690804C43617362 @default.
- W2319690804 hasConceptScore W2319690804C47558249 @default.
- W2319690804 hasConceptScore W2319690804C49105822 @default.
- W2319690804 hasConceptScore W2319690804C54355233 @default.
- W2319690804 hasConceptScore W2319690804C552990157 @default.
- W2319690804 hasConceptScore W2319690804C55493867 @default.
- W2319690804 hasConceptScore W2319690804C62520636 @default.
- W2319690804 hasConceptScore W2319690804C86803240 @default.
- W2319690804 hasConceptScore W2319690804C90583042 @default.
- W2319690804 hasConceptScore W2319690804C91881484 @default.
- W2319690804 hasIssue "2" @default.
- W2319690804 hasLocation W23196908041 @default.
- W2319690804 hasLocation W23196908042 @default.
- W2319690804 hasOpenAccess W2319690804 @default.
- W2319690804 hasPrimaryLocation W23196908041 @default.
- W2319690804 hasRelatedWork W1970008822 @default.
- W2319690804 hasRelatedWork W2016236853 @default.
- W2319690804 hasRelatedWork W2090652275 @default.
- W2319690804 hasRelatedWork W2108510473 @default.
- W2319690804 hasRelatedWork W2118971313 @default.