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• W2320092903 abstract "Ribosome profiling technology provides a means to precisely identify and quantitate sequences being translated in a given sample. Among parasites, ribosome profiling studies have been published for Trypanosoma brucei, Trypanosoma cruzi, and Plasmodium falciparum. Comparison of ribosome footprint read counts with mRNA abundance reveals the translation efficiency of individual genes, which varies between genes and across conditions. Switches between stages that have very different rates of protein production are common features of parasite life cycles. Details of stage-specific regulation of protein synthesis suggest important features of life cycles and transmission. While technologies for global enumeration of transcript abundance are well-developed, those that assess protein abundance require tailoring to penetrate to low-abundance proteins. Ribosome profiling circumvents this challenge by measuring global protein production via sequencing small mRNA fragments protected by the assembled ribosome. This powerful approach is now being applied to protozoan parasites including trypanosomes and Plasmodium. It has been used to identify new protein-coding sequences (CDSs) and clarify the boundaries of previously annotated CDSs in Trypanosoma brucei. Ribosome profiling has demonstrated that translation efficiencies vary widely between genes and, for trypanosomes at least, for the same gene across stages. The ribosomal proteins are themselves subjected to translational control, suggesting a means of reinforcing global translational regulation. While technologies for global enumeration of transcript abundance are well-developed, those that assess protein abundance require tailoring to penetrate to low-abundance proteins. Ribosome profiling circumvents this challenge by measuring global protein production via sequencing small mRNA fragments protected by the assembled ribosome. This powerful approach is now being applied to protozoan parasites including trypanosomes and Plasmodium. It has been used to identify new protein-coding sequences (CDSs) and clarify the boundaries of previously annotated CDSs in Trypanosoma brucei. Ribosome profiling has demonstrated that translation efficiencies vary widely between genes and, for trypanosomes at least, for the same gene across stages. The ribosomal proteins are themselves subjected to translational control, suggesting a means of reinforcing global translational regulation. this is the form that exists within the mammalian host, and here is restricted to the long slender forms, which are actively dividing. Unless noted, BFs are derived from infected mice. the in vitro cultured procyclic form of T. brucei. This form has been used in ribosome profiling experiments. the actively dividing form of T. brucei that resides in the tsetse fly midgut. the short regions of mRNA (∼28 nt) that assembled ribosomes protect from nuclease digestion. a technology that combines sequencing of fragments of mRNA protected by ribosomes and RNA-seq to quantitate protein production and translation efficiency globally. the common 39 nt sequence that is spliced onto all mRNAs in trypanosomatids, thereby forming the 5′-end of all mature mRNAs. the relative efficiency of translation of a specific mRNA in a given condition, defined here as the ratio between normalized ribosome footprint read counts and normalized mRNA read counts. an ORF whose start codon occurs before the start codon of the main coding sequence, but is on the same transcript." @default.
• W2320092903 created "2016-06-24" @default.
• W2320092903 creator A5043469393 @default.
• W2320092903 creator A5051357358 @default.
• W2320092903 date "2016-06-01" @default.
• W2320092903 modified "2023-09-24" @default.
• W2320092903 title "Illuminating Parasite Protein Production by Ribosome Profiling" @default.
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• W2320092903 doi "https://doi.org/10.1016/j.pt.2016.03.005" @default.
• W2320092903 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4884476" @default.
• W2320092903 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27061497" @default.
• W2320092903 hasPublicationYear "2016" @default.
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