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• W2320162489 startingPage "1497" @default.
• W2320162489 abstract "MicroRNAs (miRNAs) are post-transcriptional regulators of gene expression that are implicated in a number of disease states. MiRNAs can exist as individual entities or may be clustered and transcribed as a single polycistron. The mirn23a cluster consists of three miRNAs: miR-23a, miR-24-2, and miR-27a. Although these miRNAs are transcribed together, they often exist at varying levels in the cell. Despite the fact that the mirn23a cluster is known to play a role in a number of diseases and developmental processes, few direct targets have been identified. In this study, we examined the effects of miR-23a, miR-24-2, miR-27a, or the mirn23a cluster overexpression on the proteome of 70Z/3 pre-B lymphoblast cells. Quantitative mass spectrometry using isobaric tags for relative and absolute quantification (iTRAQ) allowed for the global profiling of cell lines after miRNA overexpression. We identified a number of targets of each miRNA that contained predicted miRNA seed sequences and are likely direct targets. In addition, we discovered a cohort of shared miRNA targets and cluster targets, demonstrating the importance of studying miRNA clusters in their entirety." @default.
• W2320162489 created "2016-06-24" @default.
• W2320162489 creator A5023288083 @default.
• W2320162489 creator A5059000079 @default.
• W2320162489 creator A5073307065 @default.
• W2320162489 date "2016-04-06" @default.
• W2320162489 modified "2023-09-27" @default.
• W2320162489 title "Evaluation of the mirn23a Cluster through an iTRAQ-based Quantitative Proteomic Approach" @default.
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• W2320162489 doi "https://doi.org/10.1021/acs.jproteome.5b01101" @default.
• W2320162489 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5305806" @default.
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• W2320162489 hasPublicationYear "2016" @default.
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