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- W2320210859 abstract "At present time, the human ACD blood preservation is ordinally permitted within 3 weeks in Japan.The purpose of this experiments are to make the more longer term preservation of blood in liquid state.In 1965, Sase reported that the denaturation of hemoglobin solution to met-hemoglobin during storage had been minimumed in oxyhemoglobin or reduced hemoglobin more than half-to-half mixture.This phenomenon might be occured in hemoglobin in Red Blood Cell during the storage.Experiment IUnder this hypothesis three kinds of ACD blood groups, first in regular venous state, second in fully oxygenated state, and third in reduced state were stored. After 2 weeks preservation in 4 to 10 centigrade room those blood were transfused in rabbits, the RBC post-transfusion survival was measured with radio-active cromium laveling method.The results of T1/2 in each oxygenated group, deoxygenated group, and venous group were 14.3 days, 6.1 days, and 11 days.Respectively, the most effective one was observed in oxygenated ACD blood group.Experiment IIExpecting the protective effect of oxydative hemolysis about RBC membrane, reduced gluthatione (GSH) (100mg/dl) was added in each three blood groups in Exp. I. After 2 weeks storage the RBC post-transfusion survival was measured.The T1/2 of the oxygenated GSH added group was prolonged to 20.3 days.The T1/2 of venous GSH added group was same to Exp. I. But the T1/2 of deoxygenated GSH added group after 3 weeks storage was prolonged to 15 days.The remarkable effect was observed in oxygenated GSH added blood group and deoxygenated GSH added blood group.Experiment IIIOxyphenarsine (0.01g/dl) which act to block the sulphohydril conpounds in blood was added in oxygenated GSH blood group.Two weeks later the blood was transfused in rabbit and RBC posttransfusion survival was measured. The T1/2 was 12 days, which resembles to that of venous blood. The results indicate the fact that SH component acts to protect the RBC membrane from denaturation. The protection was not recognized in mixed venous state." @default.
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- W2320210859 date "1970-01-01" @default.
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- W2320210859 title "OBSERVATIONS ON THE EFFECTS OF REDUCED GLUTHATION ON OXYGENATED RED-CELL PRESERVATION" @default.
- W2320210859 doi "https://doi.org/10.3925/jjtc1958.17.33" @default.
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