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- W2320227319 abstract "The acidification and bubbling method, a liquid scintillation counting (LSC) technique could yield erroneous results if the effects of suspended phytoplankton organisms on 14C counting efficiency (physical quenching, selfabsorption) are disregarded. Effects of pH change and incorporation of 4C by diatoms, blue-green and green algae on 14C counting efficiency were investigated. The paper gives experimental evidence that the suggested LSC technique does not discriminate between dissolved and particulate 4C labelled compounds, and acidification does not interfere with 4C counting efficiency. Thus selfabsorption can be neglected and quenching does not need to be considered if 4C activity of each sample is measured twice, at its natural pH and after acidification and bubbling. The paper further demonstrates, that this technique provides highly reproducible results. Therefore in primary production research, the application of the acid bubbling method, a simple, relatively inexpensive and accurate technique, is highly recommended." @default.
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- W2320227319 date "1979-01-01" @default.
- W2320227319 modified "2023-10-06" @default.
- W2320227319 title "Comments to the Acidification and Bubbling Method for Determining Phytoplankton Production" @default.
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- W2320227319 doi "https://doi.org/10.2307/3544513" @default.
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