Matches in SemOpenAlex for { <https://semopenalex.org/work/W2320304684> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2320304684 endingPage "1666" @default.
- W2320304684 startingPage "1657" @default.
- W2320304684 abstract "Staphylococcus aureus is capable of colonizing and infecting humans by its arsenal of surface-exposed and secreted proteins. Iron-limited conditions in mammalian body fluids serve as a major environmental signal to bacteria to express virulence determinants. Here we present a comprehensive, gel-free, and GeLC−MS/MS-based quantitative proteome profiling of S. aureus under this infection-relevant situation. 14N15N metabolic labeling and three complementing approaches were combined for relative quantitative analyses of surface-associated proteins. The surface-exposed and secreted proteome profiling approaches comprise trypsin shaving, biotinylation, and precipitation of the supernatant. By analysis of the outer subproteomic and cytoplasmic protein fraction, 1210 proteins could be identified including 221 surface-associated proteins. Thus, access was enabled to 70% of the predicted cell wall-associated proteins, 80% of the predicted sortase substrates, two/thirds of lipoproteins and more than 50% of secreted and cytoplasmic proteins. For iron-deficiency, 158 surface-associated proteins were quantified. Twenty-nine proteins were found in altered amounts showing particularly surface-exposed proteins strongly induced, such as the iron-regulated surface determinant proteins IsdA, IsdB, IsdC and IsdD as well as lipid-anchored iron compound-binding proteins. The work presents a crucial subject for understanding S. aureus pathophysiology by the use of methods that allow quantitative surface proteome profiling." @default.
- W2320304684 created "2016-06-24" @default.
- W2320304684 creator A5014338742 @default.
- W2320304684 creator A5039842058 @default.
- W2320304684 creator A5076044114 @default.
- W2320304684 creator A5080866705 @default.
- W2320304684 creator A5082748007 @default.
- W2320304684 date "2011-04-01" @default.
- W2320304684 modified "2023-10-17" @default.
- W2320304684 title "Quantitative Proteomic View on Secreted, Cell Surface-Associated, and Cytoplasmic Proteins of the Methicillin-Resistant Human Pathogen<i>Staphylococcus aureus</i>under Iron-Limited Conditions" @default.
- W2320304684 doi "https://doi.org/10.1021/pr1009838" @default.
- W2320304684 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21323324" @default.
- W2320304684 hasPublicationYear "2011" @default.
- W2320304684 type Work @default.
- W2320304684 sameAs 2320304684 @default.
- W2320304684 citedByCount "61" @default.
- W2320304684 countsByYear W23203046842012 @default.
- W2320304684 countsByYear W23203046842013 @default.
- W2320304684 countsByYear W23203046842014 @default.
- W2320304684 countsByYear W23203046842015 @default.
- W2320304684 countsByYear W23203046842016 @default.
- W2320304684 countsByYear W23203046842017 @default.
- W2320304684 countsByYear W23203046842018 @default.
- W2320304684 countsByYear W23203046842019 @default.
- W2320304684 countsByYear W23203046842021 @default.
- W2320304684 crossrefType "journal-article" @default.
- W2320304684 hasAuthorship W2320304684A5014338742 @default.
- W2320304684 hasAuthorship W2320304684A5039842058 @default.
- W2320304684 hasAuthorship W2320304684A5076044114 @default.
- W2320304684 hasAuthorship W2320304684A5080866705 @default.
- W2320304684 hasAuthorship W2320304684A5082748007 @default.
- W2320304684 hasConcept C104317684 @default.
- W2320304684 hasConcept C104397665 @default.
- W2320304684 hasConcept C146543888 @default.
- W2320304684 hasConcept C156399914 @default.
- W2320304684 hasConcept C165175003 @default.
- W2320304684 hasConcept C190062978 @default.
- W2320304684 hasConcept C204628709 @default.
- W2320304684 hasConcept C2776460866 @default.
- W2320304684 hasConcept C2778839769 @default.
- W2320304684 hasConcept C2779489039 @default.
- W2320304684 hasConcept C46111723 @default.
- W2320304684 hasConcept C49039625 @default.
- W2320304684 hasConcept C523546767 @default.
- W2320304684 hasConcept C54355233 @default.
- W2320304684 hasConcept C55493867 @default.
- W2320304684 hasConcept C60987743 @default.
- W2320304684 hasConcept C86803240 @default.
- W2320304684 hasConcept C89423630 @default.
- W2320304684 hasConcept C95444343 @default.
- W2320304684 hasConceptScore W2320304684C104317684 @default.
- W2320304684 hasConceptScore W2320304684C104397665 @default.
- W2320304684 hasConceptScore W2320304684C146543888 @default.
- W2320304684 hasConceptScore W2320304684C156399914 @default.
- W2320304684 hasConceptScore W2320304684C165175003 @default.
- W2320304684 hasConceptScore W2320304684C190062978 @default.
- W2320304684 hasConceptScore W2320304684C204628709 @default.
- W2320304684 hasConceptScore W2320304684C2776460866 @default.
- W2320304684 hasConceptScore W2320304684C2778839769 @default.
- W2320304684 hasConceptScore W2320304684C2779489039 @default.
- W2320304684 hasConceptScore W2320304684C46111723 @default.
- W2320304684 hasConceptScore W2320304684C49039625 @default.
- W2320304684 hasConceptScore W2320304684C523546767 @default.
- W2320304684 hasConceptScore W2320304684C54355233 @default.
- W2320304684 hasConceptScore W2320304684C55493867 @default.
- W2320304684 hasConceptScore W2320304684C60987743 @default.
- W2320304684 hasConceptScore W2320304684C86803240 @default.
- W2320304684 hasConceptScore W2320304684C89423630 @default.
- W2320304684 hasConceptScore W2320304684C95444343 @default.
- W2320304684 hasIssue "4" @default.
- W2320304684 hasLocation W23203046841 @default.
- W2320304684 hasLocation W23203046842 @default.
- W2320304684 hasOpenAccess W2320304684 @default.
- W2320304684 hasPrimaryLocation W23203046841 @default.
- W2320304684 hasRelatedWork W1121398759 @default.
- W2320304684 hasRelatedWork W1977571916 @default.
- W2320304684 hasRelatedWork W1999231385 @default.
- W2320304684 hasRelatedWork W2012732153 @default.
- W2320304684 hasRelatedWork W2119346517 @default.
- W2320304684 hasRelatedWork W2163269267 @default.
- W2320304684 hasRelatedWork W2320304684 @default.
- W2320304684 hasRelatedWork W2406787733 @default.
- W2320304684 hasRelatedWork W4220883079 @default.
- W2320304684 hasRelatedWork W2182404594 @default.
- W2320304684 hasVolume "10" @default.
- W2320304684 isParatext "false" @default.
- W2320304684 isRetracted "false" @default.
- W2320304684 magId "2320304684" @default.
- W2320304684 workType "article" @default.