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• W2320331924 endingPage "11956" @default.
• W2320331924 startingPage "11951" @default.
• W2320331924 abstract "A simple inexpensive light-emitting diode (LED)-based fluorescence detector for detection in capillary separations is described. The modular design includes a separate high power LED source, detector head, designed in the epifluorescence arrangement, and capillary detection cells. The detector head and detection cells were printed using a 3D printer and assembled with commercially available optical components. Optical fibers were used for connecting the detector head to the LED excitation source and the photodetector module. Microscope objective or high numerical aperture optical fiber were used for collection of the fluorescence emission from the fused silica separation capillary. As an example, mixture of oligosaccharides labeled by 8-aminopyrene-1,3,6-trisulfonate (APTS) was separated by capillary zone electrophoresis and detected by the described detector. The performance of the detector was compared with both a semiconductor photodiode and photomultiplier as light sensing elements. The main advantages of the 3D printed parts, compared to the more expensive alternatives from the optic component suppliers, include not only cost reduction, but also easy customization of the spatial arrangement, modularity, miniaturization, and sharing of information between laboratories for easy replication or further modifications of the detector. All information and files necessary for printing the presented detector are enclosed in the Supporting Information." @default.
• W2320331924 created "2016-06-24" @default.
• W2320331924 creator A5053594308 @default.
• W2320331924 creator A5065878600 @default.
• W2320331924 date "2014-11-26" @default.
• W2320331924 modified "2023-10-17" @default.
• W2320331924 title "Fluorescence Detector for Capillary Separations Fabricated by 3D Printing" @default.
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• W2320331924 doi "https://doi.org/10.1021/ac503678n" @default.
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• W2320331924 hasPublicationYear "2014" @default.
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